Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C3F35_RS15735 Genome accession   NZ_CP026387
Coordinates   3233336..3234313 (+) Length   325 a.a.
NCBI ID   WP_103792615.1    Uniprot ID   -
Organism   Leclercia sp. LSNIH3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3228336..3239313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F35_RS15700 (C3F35_15705) - 3228567..3229286 (+) 720 WP_103792620.1 DUF2884 domain-containing protein -
  C3F35_RS15705 (C3F35_15710) - 3229578..3229940 (-) 363 WP_103792619.1 endonuclease domain-containing protein -
  C3F35_RS15710 (C3F35_15715) hemW 3230004..3231146 (-) 1143 WP_103792618.1 radical SAM family heme chaperone HemW -
  C3F35_RS15715 (C3F35_15720) - 3231139..3231732 (-) 594 WP_103792617.1 XTP/dITP diphosphatase -
  C3F35_RS15720 (C3F35_15725) yggU 3231736..3232032 (-) 297 WP_103792616.1 DUF167 family protein YggU -
  C3F35_RS15725 (C3F35_15730) - 3232029..3232595 (-) 567 WP_032613937.1 YggT family protein -
  C3F35_RS15730 (C3F35_15735) - 3232617..3233318 (-) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  C3F35_RS15735 (C3F35_15740) pilT 3233336..3234313 (+) 978 WP_103792615.1 type IV pilus twitching motility protein PilT Machinery gene
  C3F35_RS15740 (C3F35_15745) - 3234413..3235102 (+) 690 WP_032613957.1 hypothetical protein -
  C3F35_RS15745 (C3F35_15750) ruvX 3235142..3235558 (-) 417 WP_103792614.1 Holliday junction resolvase RuvX -
  C3F35_RS15750 (C3F35_15755) - 3235558..3236118 (-) 561 WP_032613928.1 YqgE/AlgH family protein -
  C3F35_RS15755 (C3F35_15760) gshB 3236210..3237157 (-) 948 WP_032613926.1 glutathione synthase -
  C3F35_RS15760 (C3F35_15765) rsmE 3237177..3237908 (-) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C3F35_RS15765 (C3F35_15770) endA 3238020..3238727 (-) 708 WP_032613924.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35672.91 Da        Isoelectric Point: 7.0860

>NTDB_id=269078 C3F35_RS15735 WP_103792615.1 3233336..3234313(+) (pilT) [Leclercia sp. LSNIH3]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVMLLKSWLSDEQQGAWCAQGQVDFAVTLKSHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=269078 C3F35_RS15735 WP_103792615.1 3233336..3234313(+) (pilT) [Leclercia sp. LSNIH3]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGGCCGGATGTGGTGATGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGAAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGGGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTCAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAATTGCGCGATAGCGAAACGATCCGCCTTGCCCTCACCGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACCCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAGCTGGCCGGCAGTTTGCAGGCGGTGCTGGCACAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTCATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGTGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.492
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.492
  pilT Legionella pneumophila strain Lp02

48.137

99.077

0.477
  pilT Legionella pneumophila strain ERS1305867

48.137

99.077

0.477
  pilT Acinetobacter baylyi ADP1

47.095

100

0.474
  pilT Neisseria meningitidis 8013

46.951

100

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.58

97.538

0.474
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.471
  pilT Pseudomonas aeruginosa PAK

47.95

97.538

0.468
  pilT Acinetobacter nosocomialis M2

47.649

98.154

0.468
  pilT Acinetobacter baumannii strain A118

47.649

98.154

0.468
  pilT Acinetobacter baumannii D1279779

47.649

98.154

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

37.037

99.692

0.369

Multiple sequence alignment