Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NT01CX_RS06855 Genome accession   NC_008593
Coordinates   1458087..1459142 (-) Length   351 a.a.
NCBI ID   WP_011722335.1    Uniprot ID   A0Q137
Organism   Clostridium novyi NT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1453087..1464142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT01CX_RS06835 (NT01CX_2262) sigE 1453709..1454416 (-) 708 WP_011722331.1 RNA polymerase sporulation sigma factor SigE -
  NT01CX_RS06840 (NT01CX_2263) spoIIGA 1454431..1455225 (-) 795 WP_011722332.1 sigma-E processing peptidase SpoIIGA -
  NT01CX_RS06845 (NT01CX_2264) ftsZ 1455380..1456564 (-) 1185 WP_011722333.1 cell division protein FtsZ -
  NT01CX_RS06850 (NT01CX_2265) ftsA 1456580..1457833 (-) 1254 WP_011722334.1 cell division protein FtsA -
  NT01CX_RS06855 (NT01CX_2266) pilT 1458087..1459142 (-) 1056 WP_011722335.1 type IV pilus twitching motility protein PilT Machinery gene
  NT01CX_RS06860 (NT01CX_2267) sigK 1459253..1459957 (-) 705 WP_039238515.1 RNA polymerase sporulation sigma factor SigK -
  NT01CX_RS06865 (NT01CX_2268) - 1460024..1461709 (-) 1686 WP_011722337.1 penicillin-binding transpeptidase domain-containing protein -
  NT01CX_RS06870 (NT01CX_2269) udk 1461771..1462397 (-) 627 WP_011722338.1 uridine kinase -
  NT01CX_RS06875 (NT01CX_2270) - 1462401..1463627 (-) 1227 WP_011722339.1 peptidase U32 family protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38966.09 Da        Isoelectric Point: 9.3058

>NTDB_id=26906 NT01CX_RS06855 WP_011722335.1 1458087..1459142(-) (pilT) [Clostridium novyi NT]
MILLNELLKKTVEEKASDLHLTVGISPVLRVNGDLVRVGEEKLTASDTKKYAKEILGNKFDEYDSVGEIDTSISLPSVGR
FRVNIYKQRGSHALAIRAVALKIPTISQLKLPPIIKELSNKHRGLILVTGPTGSGKSTTLASIINEINTTRSAHVITLED
PIEFLHKHNKSIINQREVGSDTPSYTHALRAALREDPDVILVGEMRDLETIATAITAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPHQQQQIKIQLSNVLQGVISQQLIPRYDEKGQVASFEIMVLNSAIQNLIREGKTHQIQSLIQTGSKYGMKTMDMS
IVELYRNKMISYENALTYSVDKSMVKRMIDM

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=26906 NT01CX_RS06855 WP_011722335.1 1458087..1459142(-) (pilT) [Clostridium novyi NT]
ATGATATTATTAAATGAATTATTAAAGAAAACTGTAGAAGAGAAAGCTTCTGATTTACATTTAACCGTTGGAATTTCTCC
GGTTTTAAGGGTTAATGGAGACCTTGTAAGGGTTGGAGAAGAAAAGCTTACGGCTAGTGATACAAAAAAATATGCCAAAG
AAATATTAGGTAATAAGTTTGATGAGTATGATAGTGTTGGAGAAATAGATACATCAATATCTCTTCCAAGTGTTGGGAGA
TTTAGGGTTAATATATATAAACAAAGAGGAAGTCATGCTTTAGCTATAAGAGCCGTTGCATTAAAAATACCAACTATTAG
TCAATTAAAGTTACCACCTATAATAAAAGAGCTTAGTAATAAACATAGAGGATTAATTTTAGTTACTGGACCTACAGGAA
GTGGAAAAAGTACTACTCTTGCATCAATTATAAATGAAATAAATACAACTAGATCAGCACATGTTATTACACTAGAAGAT
CCTATAGAATTTTTACATAAACATAATAAGTCTATTATAAATCAGAGAGAAGTTGGAAGTGATACTCCTTCTTATACCCA
TGCCCTTAGAGCGGCACTTAGAGAGGATCCTGATGTTATACTAGTGGGAGAGATGAGAGATCTTGAGACTATAGCAACAG
CTATAACTGCTGCTGAAACAGGTCACTTAGTATTGTCTACATTACATACTATAGGTGCTGCCAAGACAATAGATAGAATA
ATAGATGTATTTCCTCCACACCAACAACAACAAATAAAAATACAACTTTCCAATGTACTTCAAGGGGTTATTTCTCAACA
ATTAATACCAAGATATGACGAAAAAGGACAAGTAGCTTCTTTTGAAATAATGGTGTTGAATTCAGCAATACAAAATTTAA
TTAGGGAAGGAAAAACTCATCAAATACAGTCTTTAATTCAAACAGGAAGTAAGTATGGAATGAAAACTATGGATATGAGT
ATAGTGGAGTTATATAGAAATAAAATGATTTCTTATGAAAATGCACTTACATATTCTGTTGATAAAAGTATGGTAAAAAG
AATGATAGATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0Q137

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.143

99.715

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.025

91.453

0.476
  pilT Vibrio cholerae strain A1552

52.025

91.453

0.476
  pilT Neisseria meningitidis 8013

49.85

94.872

0.473
  pilT Neisseria gonorrhoeae MS11

49.55

94.872

0.47
  pilT Legionella pneumophila strain ERS1305867

48.529

96.866

0.47
  pilT Legionella pneumophila strain Lp02

48.529

96.866

0.47
  pilT Acinetobacter nosocomialis M2

48.955

95.442

0.467
  pilT Acinetobacter baumannii strain A118

48.955

95.442

0.467
  pilT Acinetobacter baumannii D1279779

48.955

95.442

0.467
  pilT Acinetobacter baylyi ADP1

48.649

94.872

0.462
  pilT Pseudomonas aeruginosa PAK

48.348

94.872

0.459
  pilT Pseudomonas stutzeri DSM 10701

46.246

94.872

0.439
  pilU Acinetobacter baylyi ADP1

41.42

96.296

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.255

99.43

0.39
  pilU Vibrio cholerae strain A1552

39.481

98.86

0.39

Multiple sequence alignment