Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C2U43_RS04340 Genome accession   NZ_CP026238
Coordinates   859221..860201 (+) Length   326 a.a.
NCBI ID   WP_048236732.1    Uniprot ID   -
Organism   Citrobacter freundii complex sp. CFNIH9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 854221..865201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U43_RS04310 (C2U43_04315) ansB 854736..855782 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  C2U43_RS04315 (C2U43_04320) hemW 855897..857033 (-) 1137 WP_103284731.1 radical SAM family heme chaperone HemW -
  C2U43_RS04320 (C2U43_04325) - 857026..857619 (-) 594 WP_103284732.1 XTP/dITP diphosphatase -
  C2U43_RS04325 (C2U43_04330) yggU 857627..857917 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  C2U43_RS04330 (C2U43_04335) - 857914..858480 (-) 567 WP_003825417.1 YggT family protein -
  C2U43_RS04335 (C2U43_04340) - 858499..859203 (-) 705 WP_103284733.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2U43_RS04340 (C2U43_04345) pilT 859221..860201 (+) 981 WP_048236732.1 type IV pilus twitching motility protein PilT Machinery gene
  C2U43_RS04345 (C2U43_04350) ruvX 860198..860614 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  C2U43_RS04350 (C2U43_04355) - 860614..861177 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  C2U43_RS04355 (C2U43_04360) gshB 861354..862301 (-) 948 WP_003027083.1 glutathione synthase -
  C2U43_RS04360 (C2U43_04365) rsmE 862321..863052 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C2U43_RS04365 (C2U43_04370) endA 863127..863834 (-) 708 WP_103284734.1 deoxyribonuclease I -
  C2U43_RS04370 (C2U43_04375) - 863929..864426 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35813.92 Da        Isoelectric Point: 6.0355

>NTDB_id=268010 C2U43_RS04340 WP_048236732.1 859221..860201(+) (pilT) [Citrobacter freundii complex sp. CFNIH9]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWQEQGQIDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPCLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKDPVRNQLADSLRAVLSQKLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=268010 C2U43_RS04340 WP_048236732.1 859221..860201(+) (pilT) [Citrobacter freundii complex sp. CFNIH9]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCCGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCACTGGCGCTAAGATTGCTCCCGCTTGATTGTCCATGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTAGAGTACCTTAATCAGCATGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCATTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCACAGGCCATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAGGATCCGGTGCGTAACCAGTTAGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCTGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTGCCCGGCGTAATTCAAACCGGGCAACAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAGGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.364

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.061

100

0.466
  pilT Acinetobacter baylyi ADP1

45.593

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.289

100

0.457
  pilT Acinetobacter baumannii D1279779

45.289

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.985

100

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368

Multiple sequence alignment