Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C2U53_RS05140 Genome accession   NZ_CP026216
Coordinates   393572..394552 (-) Length   326 a.a.
NCBI ID   WP_104445690.1    Uniprot ID   A0A3Q8D6K3
Organism   Citrobacter sp. CFNIH10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388572..399552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U53_RS05110 (C2U53_05105) - 389334..389834 (+) 501 WP_044255652.1 SprT family zinc-dependent metalloprotease -
  C2U53_RS05115 (C2U53_05110) endA 389926..390633 (+) 708 WP_044263980.1 deoxyribonuclease I -
  C2U53_RS05120 (C2U53_05115) rsmE 390713..391444 (+) 732 WP_042998055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C2U53_RS05125 (C2U53_05120) gshB 391457..392404 (+) 948 WP_104445689.1 glutathione synthase -
  C2U53_RS05130 (C2U53_05125) - 392579..393142 (+) 564 WP_042998057.1 YqgE/AlgH family protein -
  C2U53_RS05135 (C2U53_05130) ruvX 393142..393558 (+) 417 WP_042998058.1 Holliday junction resolvase RuvX -
  C2U53_RS05140 (C2U53_05135) pilT 393572..394552 (-) 981 WP_104445690.1 type IV pilus twitching motility protein PilT Machinery gene
  C2U53_RS05145 (C2U53_05140) - 394570..395274 (+) 705 WP_042998060.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2U53_RS05150 (C2U53_05145) - 395293..395859 (+) 567 WP_042998061.1 YggT family protein -
  C2U53_RS05155 (C2U53_05150) yggU 395856..396146 (+) 291 WP_042324106.1 DUF167 family protein YggU -
  C2U53_RS05160 (C2U53_05155) - 396154..396747 (+) 594 WP_042998063.1 XTP/dITP diphosphatase -
  C2U53_RS05165 (C2U53_05160) hemW 396740..397876 (+) 1137 WP_104445691.1 radical SAM family heme chaperone HemW -
  C2U53_RS05170 (C2U53_05165) - 397908..398915 (-) 1008 WP_042998065.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35926.94 Da        Isoelectric Point: 7.0211

>NTDB_id=267834 C2U53_RS05140 WP_104445690.1 393572..394552(-) (pilT) [Citrobacter sp. CFNIH10]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTTTDITGQLADWLDEGQQAALRQNGQLDFAVSLADNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YIYTSQRCLIQQREAGLHCASFATGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEPDKQEGRFALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=267834 C2U53_RS05140 WP_104445690.1 393572..394552(-) (pilT) [Citrobacter sp. CFNIH10]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGACGCATGGAGCGTGCACCCTTTACCACAACCGACATTACCGGACAGCTGGCGGACTGGCTGGATG
AGGGGCAACAGGCGGCGTTACGACAAAATGGGCAACTGGATTTCGCGGTTTCGCTCGCGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTACGGTTGCTGCCGACGCAGTGCCCGACGCTGGAAACGCTGGG
GACGCCTTCTGTGCTGCCCGAACTGCTCCGCAGCGAGAATGGTTTGATTCTGGTGACAGGGGCGACAGGCAGCGGAAAAT
CAACTACCCTGGCGGCGATGGTCACGTGGCTGAATCAGCATATCGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATATCTACACCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGCAGGACTGCACTGCGCCTCATTTGCCACCGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGCTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGTGCCGCGCAGGCGATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTACTGTCGCAGAAACTGGAACC
CGACAAGCAGGAGGGGCGCTTTGCGCTGTATGAACTGCTGATCAACACGCCGGCAGCGGGGAATTTAATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCACAAGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q8D6K3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

42.258

95.092

0.402
  pilU Acinetobacter baylyi ADP1

38.889

99.387

0.387

Multiple sequence alignment