Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C0971_RS13250 Genome accession   NZ_CP026143
Coordinates   2611134..2612174 (-) Length   346 a.a.
NCBI ID   WP_248891967.1    Uniprot ID   -
Organism   Bacillus methanolicus strain DFS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606134..2617174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS13215 - 2606151..2606456 (-) 306 WP_248891961.1 hypothetical protein -
  C0971_RS13220 - 2606490..2606954 (-) 465 WP_248891962.1 hypothetical protein -
  C0971_RS13225 (C0971_13280) - 2606951..2607625 (-) 675 WP_248891963.1 fimbrial assembly protein -
  C0971_RS13230 (C0971_13285) pilM 2607628..2608611 (-) 984 WP_248891964.1 pilus assembly protein PilM -
  C0971_RS13235 (C0971_13290) - 2608624..2609376 (-) 753 WP_248891965.1 A24 family peptidase -
  C0971_RS13240 (C0971_13295) - 2609385..2609819 (-) 435 WP_248891966.1 type II secretion system protein -
  C0971_RS13245 (C0971_13300) - 2609927..2611132 (-) 1206 Protein_2584 type II secretion system F family protein -
  C0971_RS13250 (C0971_13305) pilT 2611134..2612174 (-) 1041 WP_248891967.1 type IV pilus twitching motility protein PilT Machinery gene
  C0971_RS13255 (C0971_13310) - 2612190..2613851 (-) 1662 WP_248891968.1 ATPase, T2SS/T4P/T4SS family -
  C0971_RS13260 (C0971_13315) - 2613875..2615095 (-) 1221 WP_248891969.1 VanW family protein -
  C0971_RS13265 (C0971_13320) - 2615259..2615868 (-) 610 Protein_2588 PRC-barrel domain-containing protein -
  C0971_RS13270 (C0971_13325) - 2615859..2616650 (-) 792 WP_283108194.1 vWA domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38405.61 Da        Isoelectric Point: 9.2898

>NTDB_id=267530 C0971_RS13250 WP_248891967.1 2611134..2612174(-) (pilT) [Bacillus methanolicus strain DFS2]
MKNKIDLLLRAGFELKASDIHLTVGVPPVFRIHGELKRYGKDPLAPSDTEGMARAIIPENMWEQFKKKGELDFSYGIPGI
SRFRINAYFQRSCIAMAIRIVPTKIPTLQELELPPVLAKIAEKPQGLVLVTGPTGSGKSTTLAAMINHMNQTMRKHIITL
EDPIEYLHKHGTCIIDQREVGFDTNNFANGLRASLRQDPDVIMVGEMRDLETIQTAITAAETGHLVFGTLHTASAPDTIN
RIIDVFPPSQQAQIRFQLASVLVAIISQRLFLKADKKGRKAATEILLNNSAVANLIRNEKIHQIVNIMQTSRSQGMHTLA
TSIKELVESGQVSAEETMPYLLEKVN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=267530 C0971_RS13250 WP_248891967.1 2611134..2612174(-) (pilT) [Bacillus methanolicus strain DFS2]
ATGAAAAACAAAATTGATTTATTGCTTCGTGCCGGTTTTGAATTAAAAGCTTCAGATATTCATCTTACCGTCGGGGTTCC
GCCGGTCTTCCGCATTCATGGCGAATTGAAAAGATATGGAAAGGACCCGCTCGCACCTTCCGATACGGAAGGTATGGCAA
GGGCAATCATTCCGGAAAATATGTGGGAACAATTTAAGAAAAAAGGAGAGCTTGATTTCTCATACGGCATTCCCGGAATT
TCCCGTTTTCGAATCAATGCGTATTTTCAAAGGTCGTGCATTGCCATGGCCATTCGCATCGTTCCGACAAAAATTCCGAC
ACTGCAGGAATTGGAACTGCCGCCTGTCCTTGCAAAAATTGCTGAAAAGCCTCAAGGGCTCGTTCTTGTCACCGGACCGA
CTGGGAGCGGCAAATCGACAACACTTGCAGCGATGATCAATCATATGAATCAAACGATGCGAAAACATATCATTACCCTT
GAAGATCCGATTGAATATTTGCATAAGCATGGGACATGCATTATTGACCAGAGGGAAGTCGGATTTGATACAAATAATTT
TGCAAACGGATTAAGGGCATCGCTCAGACAGGATCCTGATGTCATCATGGTCGGAGAAATGCGTGATCTTGAAACGATTC
AAACTGCTATCACTGCAGCGGAAACAGGGCATCTTGTGTTTGGTACTCTACATACGGCTAGTGCTCCGGACACAATTAAC
AGAATTATCGATGTATTTCCGCCTTCCCAGCAAGCCCAAATAAGATTTCAGCTTGCATCCGTTCTTGTGGCGATCATTTC
TCAACGTTTATTTCTGAAAGCAGACAAAAAAGGACGAAAAGCCGCAACTGAAATTTTACTTAACAATTCAGCAGTTGCAA
ATTTAATCAGAAATGAGAAGATCCACCAAATCGTCAACATCATGCAAACATCAAGATCCCAAGGAATGCATACGCTTGCA
ACGAGCATAAAAGAGCTTGTTGAGTCGGGCCAAGTGTCTGCTGAAGAAACGATGCCTTATTTATTGGAAAAGGTGAATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.648

92.775

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.606

95.376

0.483
  pilT Vibrio cholerae strain A1552

50.606

95.376

0.483
  pilT Neisseria meningitidis 8013

47.289

95.954

0.454
  pilT Neisseria gonorrhoeae MS11

46.988

95.954

0.451
  pilT Acinetobacter baylyi ADP1

46.933

94.22

0.442
  pilT Acinetobacter nosocomialis M2

47.95

91.618

0.439
  pilT Acinetobacter baumannii D1279779

47.95

91.618

0.439
  pilT Acinetobacter baumannii strain A118

47.95

91.618

0.439
  pilT Legionella pneumophila strain ERS1305867

46.626

94.22

0.439
  pilT Legionella pneumophila strain Lp02

46.626

94.22

0.439
  pilT Pseudomonas aeruginosa PAK

47.649

92.197

0.439
  pilT Pseudomonas stutzeri DSM 10701

47.022

92.197

0.434
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.665

0.393
  pilU Vibrio cholerae strain A1552

41.176

93.353

0.384
  pilU Acinetobacter baylyi ADP1

39.877

94.22

0.376

Multiple sequence alignment