Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AXA56_RS09130 Genome accession   NZ_CP031609
Coordinates   1843032..1844012 (+) Length   326 a.a.
NCBI ID   WP_116836331.1    Uniprot ID   -
Organism   Escherichia coli strain N3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1838032..1849012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXA56_RS09100 (AXA56_09100) yggM 1838633..1839640 (+) 1008 WP_116836329.1 DUF1202 family protein -
  AXA56_RS09105 (AXA56_09105) hemW 1839709..1840845 (-) 1137 WP_116836330.1 radical SAM family heme chaperone HemW -
  AXA56_RS09110 (AXA56_09110) rdgB 1840838..1841431 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  AXA56_RS09115 (AXA56_09115) yggU 1841439..1841729 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  AXA56_RS09120 (AXA56_09120) yggT 1841726..1842292 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  AXA56_RS09125 (AXA56_09125) yggS 1842310..1843014 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  AXA56_RS09130 (AXA56_09130) pilT 1843032..1844012 (+) 981 WP_116836331.1 type IV pilus twitching motility protein PilT Machinery gene
  AXA56_RS09135 (AXA56_09135) ruvX 1844127..1844543 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  AXA56_RS09140 (AXA56_09140) yqgE 1844543..1845106 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  AXA56_RS09145 (AXA56_09145) gshB 1845215..1846165 (-) 951 WP_116836332.1 glutathione synthase -
  AXA56_RS09150 (AXA56_09150) rsmE 1846178..1846909 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AXA56_RS09155 (AXA56_09155) endA 1846989..1847696 (-) 708 WP_000286497.1 deoxyribonuclease I -
  AXA56_RS09160 (AXA56_09160) yggI 1847791..1848288 (-) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36003.27 Da        Isoelectric Point: 6.1759

>NTDB_id=266455 AXA56_RS09130 WP_116836331.1 1843032..1844012(+) (pilT) [Escherichia coli strain N3]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGLSLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPME
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLKVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=266455 AXA56_RS09130 WP_116836331.1 1843032..1844012(+) (pilT) [Escherichia coli strain N3]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTCTTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGAGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTATGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTGGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACATACGCGTGGTGCGGCCCAGGCAGTTGAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTAAAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAACAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365

Multiple sequence alignment