Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SSA_RS11360 Genome accession   NC_009009
Coordinates   2319502..2321184 (-) Length   560 a.a.
NCBI ID   WP_002899360.1    Uniprot ID   A3CR73
Organism   Streptococcus sanguinis SK36     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2314502..2326184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSA_RS11330 (SSA_2312) - 2314883..2315371 (-) 489 WP_011837686.1 hypothetical protein -
  SSA_RS11335 (SSA_2313) - 2315495..2315938 (-) 444 WP_011837687.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SSA_RS11340 (SSA_2314) - 2315976..2316425 (-) 450 WP_011837688.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SSA_RS11345 (SSA_2315) - 2316577..2317035 (-) 459 WP_011837689.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SSA_RS11350 (SSA_2316) - 2317206..2318432 (-) 1227 WP_011837690.1 type II secretion system F family protein -
  SSA_RS11355 (SSA_2317) pilT 2318436..2319500 (-) 1065 WP_011837691.1 type IV pilus twitching motility protein PilT Machinery gene
  SSA_RS11360 (SSA_2318) pilF 2319502..2321184 (-) 1683 WP_002899360.1 GspE/PulE family protein Machinery gene
  SSA_RS11365 (SSA_2320) - 2322392..2325886 (-) 3495 WP_002917982.1 LPXTG cell wall anchor domain-containing protein -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62195.28 Da        Isoelectric Point: 4.9464

>NTDB_id=26348 SSA_RS11360 WP_002899360.1 2319502..2321184(-) (pilF) [Streptococcus sanguinis SK36]
MALIAILVQFNLITAAQKEEILQDMPQSNMQLERYLISKGYVTEEDMLKVMSYYYRVPHVNLSQFVIEKEAVEKVSEKVA
KRHGLIPISFTDGEEGEEPKLVVAMADPSNYIALDDVKIVSKMAVEPYVTFRDDIEKYIDQYYSKGEEAQQAATEIEGFN
VDEEIVEEDLEIKNAPVVRLIDSIISQAIKTRTSDIHIEPFEKVVRVRFRVDGTLVENMQLKANAHSAIATRIKIMSGLD
IAERRIPQDGRIETTIDGKEVDMRVSVLPTVFGEKIVIRILSRNATLLSKEELGFSPTNQKLFEDILKAPEGIILLTGPT
GSGKTTTLYTALRELNDVGKNIITVEDPVEYRLEGVNQVQVNNKAGLTFASGLRSILRQDPDIVLLGEIRDEETASIAVR
AAITGHVVLSTIHTNDTASTVNRLVDMGIKPYLVSTATVGIIAQRLIKRICPKCKTEYTVETNEHAGIGIHKGDTLYRGR
GCNYCSGTGYYGRIAIHEIMAVTREIKSLINDGGTTSQLRAEAKKNGMRDLAEEAIDIAKQGTTTIEEAMKIAFSLEGEV

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=26348 SSA_RS11360 WP_002899360.1 2319502..2321184(-) (pilF) [Streptococcus sanguinis SK36]
ATGGCACTAATCGCTATATTGGTTCAATTCAATCTGATAACGGCAGCTCAAAAAGAAGAAATTCTTCAAGATATGCCACA
ATCAAACATGCAGTTAGAGAGATATCTAATCAGCAAGGGCTATGTGACAGAAGAAGATATGCTGAAAGTCATGAGTTACT
ATTACCGTGTACCTCATGTCAATCTATCACAGTTTGTGATAGAAAAGGAAGCTGTCGAGAAGGTTTCAGAAAAAGTTGCT
AAACGTCATGGATTGATTCCGATCTCTTTCACAGATGGGGAAGAGGGAGAAGAACCCAAGTTAGTAGTTGCGATGGCAGA
CCCAAGCAACTATATCGCCCTAGACGACGTTAAAATCGTTTCTAAGATGGCGGTAGAACCTTATGTAACTTTTCGGGATG
ATATTGAGAAGTATATTGATCAATACTATTCTAAAGGAGAAGAAGCCCAACAGGCAGCGACCGAAATCGAAGGTTTTAAT
GTGGACGAGGAGATCGTCGAAGAAGATCTCGAAATCAAAAACGCTCCGGTTGTACGTTTGATTGACTCGATTATTAGTCA
GGCAATCAAGACGCGGACCAGCGATATCCATATTGAGCCCTTTGAAAAAGTTGTTCGTGTTCGTTTCCGGGTTGACGGGA
CTTTGGTGGAAAACATGCAGTTGAAAGCCAATGCTCACTCAGCCATTGCGACACGGATTAAGATCATGAGTGGTCTGGAC
ATTGCCGAGCGGCGGATTCCTCAGGATGGACGGATTGAAACAACCATCGACGGCAAAGAAGTCGATATGCGGGTTTCAGT
CTTGCCTACTGTATTCGGTGAAAAAATCGTTATTCGGATTTTGAGCCGGAATGCAACCCTCCTCAGTAAAGAGGAGCTGG
GATTCTCACCAACCAATCAGAAGCTCTTTGAAGATATTCTTAAGGCGCCAGAAGGCATTATCTTGCTGACTGGTCCTACA
GGAAGCGGAAAGACAACCACTCTTTACACAGCGCTCCGTGAGCTCAATGATGTAGGAAAAAATATCATCACCGTTGAAGA
TCCGGTTGAGTACCGATTGGAAGGGGTCAACCAAGTTCAGGTAAATAATAAAGCTGGACTGACCTTCGCGAGTGGTTTGA
GAAGTATCCTGCGTCAGGACCCGGACATCGTTCTCTTGGGGGAAATTCGGGACGAAGAGACAGCTAGTATCGCGGTTCGG
GCCGCTATCACTGGTCACGTCGTTCTCTCGACTATCCACACCAATGACACAGCTAGTACAGTCAACCGTTTGGTCGATAT
GGGCATCAAGCCTTATCTGGTCTCAACGGCGACTGTCGGTATTATCGCACAGCGCTTGATTAAGCGTATCTGTCCTAAGT
GTAAGACTGAGTATACTGTAGAAACAAATGAACATGCAGGTATCGGTATTCACAAGGGAGACACCTTGTATCGCGGACGC
GGCTGCAACTACTGCAGCGGTACGGGATACTACGGACGTATAGCTATCCACGAAATCATGGCAGTGACAAGGGAGATTAA
ATCACTAATTAATGATGGTGGAACAACGTCACAGCTTCGAGCAGAAGCTAAGAAAAATGGTATGCGAGACTTGGCGGAGG
AGGCCATTGATATCGCAAAACAGGGGACTACAACGATTGAAGAAGCAATGAAGATTGCCTTCAGTCTAGAAGGGGAAGTT
TAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-148)

Type II/IV secretion system protein (T2SSE)

(184-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3CR73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

39.711

98.929

0.393
  pilB Vibrio cholerae strain A1552

38.693

100

0.391
  pilB Vibrio parahaemolyticus RIMD 2210633

38.693

100

0.391
  pilB Legionella pneumophila strain ERS1305867

38.324

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.241

99.464

0.38
  pilB Vibrio campbellii strain DS40M4

37.699

100

0.38
  pilF Neisseria gonorrhoeae MS11

37.814

99.643

0.377
  pilB Acinetobacter baylyi ADP1

36.972

100

0.375
  pilB Acinetobacter baumannii D1279779

36.918

99.643

0.368

Multiple sequence alignment