Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C0J56_RS28295 Genome accession   NZ_CP025542
Coordinates   6239908..6240942 (-) Length   344 a.a.
NCBI ID   WP_134926226.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain 2P24     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6234908..6245942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0J56_RS28265 (C0J56_28255) ruvX 6234973..6235410 (+) 438 WP_134926222.1 Holliday junction resolvase RuvX -
  C0J56_RS28270 (C0J56_28260) pyrR 6235496..6236002 (+) 507 WP_134926223.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C0J56_RS28275 (C0J56_28265) - 6236027..6237031 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  C0J56_RS28280 (C0J56_28270) - 6237028..6238299 (+) 1272 WP_134926224.1 dihydroorotase -
  C0J56_RS28285 (C0J56_28275) - 6238432..6238860 (-) 429 WP_053188929.1 TM2 domain-containing protein -
  C0J56_RS28290 (C0J56_28280) - 6239111..6239722 (+) 612 WP_134926225.1 C40 family peptidase -
  C0J56_RS28295 (C0J56_28285) pilT 6239908..6240942 (-) 1035 WP_134926226.1 type IV pilus twitching motility protein PilT Machinery gene
  C0J56_RS28300 (C0J56_28290) - 6240996..6241682 (+) 687 WP_134926227.1 YggS family pyridoxal phosphate-dependent enzyme -
  C0J56_RS28305 (C0J56_28295) proC 6241718..6242536 (+) 819 WP_134926228.1 pyrroline-5-carboxylate reductase -
  C0J56_RS28310 (C0J56_28300) - 6242547..6243137 (+) 591 WP_109752399.1 YggT family protein -
  C0J56_RS28315 (C0J56_28305) - 6243143..6243433 (+) 291 WP_116833661.1 DUF167 domain-containing protein -
  C0J56_RS28320 (C0J56_28310) - 6243642..6244781 (+) 1140 WP_134926229.1 homoserine O-acetyltransferase -
  C0J56_RS28325 (C0J56_28315) metW 6244789..6245409 (+) 621 WP_109752405.1 methionine biosynthesis protein MetW -
  C0J56_RS28330 (C0J56_28320) - 6245435..6245869 (+) 435 WP_134926230.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38095.72 Da        Isoelectric Point: 7.0158

>NTDB_id=262471 C0J56_RS28295 WP_134926226.1 6239908..6240942(-) (pilT) [Pseudomonas fluorescens strain 2P24]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDHLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVSKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=262471 C0J56_RS28295 WP_134926226.1 6239908..6240942(-) (pilT) [Pseudomonas fluorescens strain 2P24]
ATGGATATCACTGAGTTACTGGCGTTCAGCGCCAAACAGGGCGCGTCGGACCTGCACCTGTCCGCCGGGTTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGCATCAACCTGCCGGCGCTGGACCACAAGCAGGTCCATGAACTGATCTACGACA
TCATGAATGACCGGCAACGGGTAGATTACGAAAAGTTTCTGGAGACCGACTTTTCCTTCGACGTGCCGGGTGTGGCGCGG
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGTGCGGGGGCGGTATTCCGGACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAAGTGTTTCGCAAAGTGACCGAAGCGCCGCGTGGGCTGGTGTTGGTGACCGGGCCTACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCCATGATCGATCACTTGAACAGCCACAAGCACCATCACATTCTCACCATCGAAGAC
CCCATCGAATTCGTTCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCAGGGTTTCTCCAC
GGCCCTGCGTTCGGCCCTGCGGGAGGATCCGGACGTGATCCTGGTAGGGGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGGCATCTGGTGTTCGGCACACTGCACACCACATCGGCGGCCAAGACCATTGACCGGGTG
GTGGACGTGTTTCCCGGTGACGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAATCGTTGCAGGCGGTGATCTCCCAGAC
GCTGGTCAAGAAGATCGGCGGCGGTCGGATCGCGGCGCATGAAATCATGCTGGGAACCTCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGTGGTTCGCTGGGGATGCAGACGCTGGATATGTGTTTG
AAGGATTTGGTGAGCAAGGGCTTGATCAGCCGCGAACATGCCCGGGAGCGGGCGCGTACACCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.372

100

0.884
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

71.893

98.256

0.706
  pilT Legionella pneumophila strain ERS1305867

71.893

98.256

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

65.217

100

0.654
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

39.42

100

0.395

Multiple sequence alignment