Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DGEO_RS03930 Genome accession   NC_008025
Coordinates   255238..256449 (+) Length   403 a.a.
NCBI ID   WP_011529413.1    Uniprot ID   -
Organism   Deinococcus geothermalis DSM 11300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250238..261449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGEO_RS03910 (Dgeo_0260) - 250238..251023 (-) 786 WP_011529409.1 enoyl-ACP reductase -
  DGEO_RS03915 (Dgeo_0261) pgeF 251130..251897 (+) 768 WP_011529410.1 peptidoglycan editing factor PgeF -
  DGEO_RS03920 (Dgeo_0262) - 251894..252394 (+) 501 WP_011529411.1 YqeG family HAD IIIA-type phosphatase -
  DGEO_RS03925 (Dgeo_0263) pilB 252422..255097 (+) 2676 WP_011529412.1 type II/IV secretion system protein Machinery gene
  DGEO_RS03930 (Dgeo_0264) pilT 255238..256449 (+) 1212 WP_011529413.1 type IV pilus twitching motility protein PilT Machinery gene
  DGEO_RS03935 (Dgeo_0265) - 256472..257845 (-) 1374 WP_011529414.1 DUF4403 family protein -
  DGEO_RS03945 (Dgeo_0266) - 257960..259321 (+) 1362 WP_371414734.1 IS66-like element ISDge4 family transposase -
  DGEO_RS03950 (Dgeo_0267) - 259414..259839 (+) 426 WP_011529415.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  DGEO_RS03955 (Dgeo_0268) - 259878..261005 (+) 1128 WP_011529416.1 nitric oxide synthase oxygenase -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43779.08 Da        Isoelectric Point: 9.0699

>NTDB_id=25877 DGEO_RS03930 WP_011529413.1 255238..256449(+) (pilT) [Deinococcus geothermalis DSM 11300]
MSHVPADITDILRFAAEKGASDVILTAGLPPQFKLSGVYETHGFAPLAATDTRKLMYSMMNERQQRIFEEKRELDFSFAL
GERARFRVNAFMQRGFVGGVLRLIPTQIRSAQEMGLPPHVIEIANAPRGLVLVTGPTGSGKSTTLAAMIDYINSTKKLHI
VTIEDPIEFMHGHKQSIVNQREVGSDTLSFQDALRAALRQAPDVILVGEMRDYETIRAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQGQIRVQLANNLVAVMTQQLLPRLDGLGRVLAYELLIANPAVRALIREGKTYQIVSTMQTGAREGMV
TMDAYLANLYRRRIISYDTGVARAVDPKEFARLANDPNTGVGGATYTPPAGSTSPTLGSGTNVGRTATPSTPPAGGNPPF
GRR

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=25877 DGEO_RS03930 WP_011529413.1 255238..256449(+) (pilT) [Deinococcus geothermalis DSM 11300]
ATGTCCCACGTGCCTGCCGACATCACCGATATCCTCCGCTTTGCCGCCGAAAAGGGCGCCTCCGACGTGATTCTGACTGC
CGGCCTGCCGCCGCAGTTCAAGCTGAGCGGCGTGTACGAGACGCACGGCTTTGCGCCGCTCGCCGCGACCGACACCCGCA
AGCTGATGTACTCGATGATGAATGAACGTCAGCAGCGCATCTTTGAAGAAAAGCGCGAGCTGGACTTCTCGTTTGCGCTG
GGCGAGCGAGCGCGCTTTCGCGTGAATGCCTTTATGCAGCGCGGTTTTGTGGGCGGCGTACTGCGGCTGATTCCCACCCA
GATCAGGAGTGCCCAGGAGATGGGTCTCCCGCCACACGTGATCGAGATCGCCAACGCGCCGCGCGGCTTGGTGCTGGTGA
CTGGGCCGACCGGCTCCGGCAAAAGCACCACGCTGGCGGCCATGATCGACTACATCAACAGCACCAAGAAGCTGCATATC
GTGACCATCGAGGACCCGATTGAGTTCATGCACGGGCACAAGCAGAGCATCGTGAATCAGCGTGAGGTGGGGTCGGACAC
CCTGAGCTTCCAGGACGCGTTGCGGGCCGCGCTGCGCCAGGCTCCCGACGTGATCTTGGTGGGGGAAATGCGCGACTACG
AGACCATCCGCGCCGCCGTCACCGCTGCAGAAACGGGGCACCTGGTCATGGGCACCCTGCACACCAACTCGGCGCCCGAA
TCCATCGACCGTATCGTGGACGTGTTTCCCGAAGAGCAGCAGGGGCAGATCCGGGTCCAGCTCGCCAACAACCTGGTCGC
CGTGATGACCCAGCAGCTTCTGCCCCGGCTGGATGGGCTGGGCCGCGTGCTGGCCTACGAACTGCTGATTGCCAACCCGG
CGGTGCGGGCCCTGATTCGCGAGGGCAAGACCTACCAGATCGTAAGCACCATGCAGACGGGGGCGCGGGAAGGTATGGTC
ACCATGGACGCCTACCTGGCGAACCTCTACCGCCGCCGCATCATCAGCTACGACACTGGCGTCGCGCGGGCCGTTGATCC
CAAAGAATTTGCCCGCCTCGCCAATGATCCGAACACCGGGGTGGGCGGGGCAACCTACACTCCCCCTGCTGGGAGCACCT
CCCCGACGTTGGGCAGCGGCACGAACGTAGGGCGCACGGCAACGCCCTCCACGCCCCCAGCGGGTGGCAATCCCCCGTTC
GGACGACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

80.829

95.782

0.774
  pilT Acinetobacter baylyi ADP1

52.616

85.36

0.449
  pilT Pseudomonas aeruginosa PAK

52.326

85.36

0.447
  pilT Pseudomonas stutzeri DSM 10701

52.326

85.36

0.447
  pilT Legionella pneumophila strain ERS1305867

52.035

85.36

0.444
  pilT Legionella pneumophila strain Lp02

52.035

85.36

0.444
  pilT Acinetobacter nosocomialis M2

52.035

85.36

0.444
  pilT Acinetobacter baumannii D1279779

52.035

85.36

0.444
  pilT Acinetobacter baumannii strain A118

52.035

85.36

0.444
  pilT Neisseria meningitidis 8013

51.884

85.608

0.444
  pilT Neisseria gonorrhoeae MS11

51.163

85.36

0.437
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.76

81.638

0.414
  pilT Vibrio cholerae strain A1552

50.76

81.638

0.414
  pilU Pseudomonas stutzeri DSM 10701

45.808

82.878

0.38
  pilU Acinetobacter baylyi ADP1

42.982

84.864

0.365

Multiple sequence alignment