Detailed information NTG0025876

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DGEO_RS03925
• Genome accession: NC_008025
• Coordinates: 252422..255097 (+)
• Length: 891 a.a.
• NCBI ID: WP_011529412.1
• Uniprot ID: A0A4R5GBR1
• Organism: Deinococcus geothermalis DSM 11300
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 247422..260097

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DGEO_RS03895 (Dgeo_0257)	-	248070..248672 (-)	603	WP_011529406.1	DinB family protein	-
DGEO_RS03900 (Dgeo_0258)	-	248706..249590 (-)	885	WP_041221496.1	acyltransferase	-
DGEO_RS03905 (Dgeo_0259)	-	249780..250211 (-)	432	WP_011529408.1	isochorismatase family protein	-
DGEO_RS03910 (Dgeo_0260)	-	250238..251023 (-)	786	WP_011529409.1	enoyl-ACP reductase	-
DGEO_RS03915 (Dgeo_0261)	pgeF	251130..251897 (+)	768	WP_011529410.1	peptidoglycan editing factor PgeF	-
DGEO_RS03920 (Dgeo_0262)	-	251894..252394 (+)	501	WP_011529411.1	YqeG family HAD IIIA-type phosphatase	-
DGEO_RS03925 (Dgeo_0263)	pilB	252422..255097 (+)	2676	WP_011529412.1	type II/IV secretion system protein	Machinery gene
DGEO_RS03930 (Dgeo_0264)	pilT	255238..256449 (+)	1212	WP_011529413.1	type IV pilus twitching motility protein PilT	Machinery gene
DGEO_RS03935 (Dgeo_0265)	-	256472..257845 (-)	1374	WP_011529414.1	DUF4403 family protein	-
DGEO_RS03945 (Dgeo_0266)	-	257960..259321 (+)	1362	WP_371414734.1	IS66-like element ISDge4 family transposase	-
DGEO_RS03950 (Dgeo_0267)	-	259414..259839 (+)	426	WP_011529415.1	secondary thiamine-phosphate synthase enzyme YjbQ	-

Sequence

Protein

• Length: 891 a.a.
• Molecular weight: 96907.94 Da
• Isoelectric Point: 4.9525

>NTDB_id=25876 DGEO_RS03925 WP_011529412.1 252422..255097(+) (pilB) [Deinococcus geothermalis DSM 11300]
MALSIADRRLGAILLEQGYVTDTDLQKALVRHAEVGGRLADILIESGQVGEKRIARAIEEALGIPLVNLLVVTPDAAALA
AIRAETAKQMQAFPFALEGQTLRVALVDPLSSVAIEALEDDSGLNIEPYQALRDQVLWSIATYYPELGLMPVLPEGAAGS
SESGGKLGQRLISRGLINEAQLQVALDVQQQTGEALGHILVTQGLLSEDQLYEVLAEQAGAVYLRNPRDFQPGEEVLGSL
LRADALRLSAVPVDETAQGVTVVVSDPRRRDELEALIGRPVQLVLARPGDVEALIERYYPQRGRLGEQMVQQGSLSRAQL
REALQVQARGGKVKPLGEVIVELGFARAEEIDAALQKQNAGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEYLDPVQNPP
DPQVALMIPEATARRYTVVPVRLQGEALVVAMKDPRNVFALDDLKLITGREIVPAVMSEKDIVRLIERYFGNQDMANLNQ
RLAAESKTREARKEADLDFSAGLDDNAVVRVVDNLIREAALQEASDIHIEPTESAVRVRYRVDGALREQPELPKGSAQSI
LARIKIMGGLDIAERRVPQDGRIRFRKGSIDLDLRLSTLPTVYGEKAVMRLLQKAENIPEVEQLGFSEHNFGRYLEVIEK
PNGIFLITGPTGSGKSFTSFSTLKRIARPEKNTTTIEDPIEYEIPGIVQSQVNVAAGMTFARALRAFLRQDPDIIFVGEI
RDTETAKIATEAALTGHLVLATLHTNDAPGAITRLSEMGVEHFNISASVVGVLAQRLVRRVCTECKQPTNADPEVLRRLG
LSEAEVRGATLMRGSGCPRCGGTGYKGRMGIHELMVIDDPLRRAIAAGRTATELRDVAMQESGMRTLRQDGIEKALAGLT
TLEEVLAVTSS

Nucleotide

• Length: 2676 bp

>NTDB_id=25876 DGEO_RS03925 WP_011529412.1 252422..255097(+) (pilB) [Deinococcus geothermalis DSM 11300]
GTGGCACTCTCGATTGCTGACCGGCGCCTAGGCGCGATTCTGCTTGAGCAGGGGTACGTGACCGACACGGACCTGCAAAA
GGCGCTGGTGCGCCATGCCGAGGTGGGTGGTCGCCTGGCGGATATTCTGATTGAGTCCGGACAGGTGGGCGAAAAGCGGA
TCGCCCGCGCCATCGAAGAAGCACTCGGTATTCCGCTGGTGAACCTCTTGGTGGTCACGCCGGATGCCGCGGCCTTGGCG
GCGATTCGGGCGGAGACGGCCAAGCAGATGCAGGCTTTTCCTTTCGCGCTGGAGGGGCAGACCCTGCGGGTGGCGCTGGT
GGACCCACTGTCCAGCGTGGCGATCGAGGCGCTGGAAGACGACAGTGGCCTGAACATCGAGCCGTATCAGGCCCTGCGCG
ATCAGGTGCTGTGGTCTATTGCGACGTATTACCCTGAACTCGGACTGATGCCGGTGTTGCCTGAAGGCGCCGCCGGCAGC
TCGGAGAGTGGGGGAAAGCTGGGGCAACGGCTGATCAGTCGGGGCCTGATCAACGAGGCGCAGCTGCAGGTCGCGCTCGA
TGTCCAGCAGCAGACGGGGGAGGCGCTGGGCCACATCCTGGTCACGCAGGGCCTGCTCAGCGAGGATCAACTCTACGAGG
TCCTGGCCGAACAGGCGGGCGCGGTGTACCTGCGCAACCCCCGCGACTTCCAGCCGGGCGAGGAGGTGCTGGGCAGCCTG
CTGCGCGCCGATGCTCTCCGCCTCTCGGCGGTGCCGGTGGACGAAACCGCGCAGGGCGTGACGGTCGTCGTGAGTGATCC
CCGCCGCCGCGACGAGCTGGAGGCGCTGATTGGGCGCCCGGTGCAGCTGGTGCTCGCCCGGCCAGGGGACGTGGAGGCGC
TGATCGAGCGCTACTACCCGCAGCGGGGCCGCCTGGGCGAGCAGATGGTCCAGCAGGGGAGCCTGTCGCGGGCACAGCTG
CGAGAAGCCCTGCAGGTGCAGGCGCGTGGTGGCAAAGTTAAGCCGCTGGGCGAGGTGATTGTCGAACTGGGGTTTGCGCG
CGCCGAGGAGATCGATGCGGCCCTCCAGAAGCAGAACGCGGGCGGGGGCCGCCTGGAAGACACCCTGGTGCAGTCGGGCA
AGCTCAGCCCCGAGATGCTGGCGCGTTCGCTGGCGGCCCAACTGGGCTACGAGTACCTCGACCCGGTGCAGAATCCACCT
GACCCCCAGGTCGCGCTGATGATTCCCGAGGCGACGGCGCGGCGCTACACGGTGGTGCCGGTGCGCCTGCAGGGTGAGGC
GCTCGTCGTCGCGATGAAGGACCCGCGTAACGTCTTTGCGCTCGACGACCTGAAACTCATCACCGGGCGGGAGATTGTCC
CGGCGGTGATGTCGGAAAAGGACATCGTCCGCCTGATCGAGCGTTACTTCGGCAACCAGGACATGGCGAACCTCAACCAG
CGCCTGGCGGCCGAGAGCAAGACCCGTGAGGCCCGCAAGGAGGCTGACCTCGACTTCTCGGCGGGACTGGACGACAACGC
TGTCGTGCGGGTGGTGGACAACCTGATCCGCGAGGCCGCCTTGCAGGAGGCTTCGGACATCCACATCGAGCCCACCGAGT
CCGCTGTGCGGGTGCGCTACCGCGTGGACGGCGCCCTGCGCGAGCAGCCCGAACTTCCCAAGGGAAGCGCCCAGAGCATC
CTGGCCCGTATCAAGATCATGGGTGGGCTGGACATCGCAGAAAGGCGCGTGCCGCAAGACGGGCGCATCCGCTTCCGCAA
GGGCAGCATTGACCTCGACCTGCGTCTCTCGACCCTGCCGACCGTCTACGGCGAAAAAGCCGTGATGAGGTTGCTGCAAA
AGGCCGAGAACATTCCGGAAGTCGAGCAGCTGGGCTTTTCCGAACACAACTTCGGGCGGTATCTGGAGGTGATCGAAAAG
CCCAACGGCATCTTTTTGATCACCGGGCCGACAGGTTCGGGCAAGTCGTTTACCTCCTTTTCTACCCTCAAGCGCATCGC
CCGCCCGGAAAAGAACACGACCACCATCGAGGACCCCATTGAGTACGAGATTCCGGGCATCGTCCAGTCACAGGTGAATG
TTGCGGCGGGCATGACCTTTGCCCGCGCCCTGCGCGCTTTCCTGCGCCAGGACCCCGACATTATCTTTGTGGGCGAAATC
CGCGACACCGAGACCGCCAAGATCGCCACCGAGGCAGCGCTGACCGGCCACCTCGTGCTCGCGACCCTGCACACCAACGA
CGCGCCCGGCGCAATCACTCGCCTCTCGGAGATGGGCGTCGAGCACTTCAACATCTCCGCCTCGGTGGTGGGGGTGCTGG
CGCAGCGTCTGGTTCGGCGCGTTTGCACCGAATGCAAGCAGCCCACCAACGCCGACCCCGAAGTGCTGCGCCGCCTGGGC
CTGAGCGAAGCCGAGGTGCGCGGCGCAACCCTGATGCGCGGTTCCGGCTGCCCCCGCTGCGGCGGCACCGGCTACAAGGG
CCGGATGGGTATCCACGAGCTGATGGTGATTGATGACCCGCTGCGCCGCGCCATCGCGGCCGGGCGCACTGCCACCGAGC
TGCGCGACGTGGCGATGCAGGAAAGCGGCATGCGTACCCTGCGCCAAGACGGCATCGAGAAGGCCCTCGCGGGCCTCACC
ACCCTGGAAGAGGTGTTGGCGGTGACGAGCAGCTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-474)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II/IV secretion system protein (T2SSE) (515-780)

3D structure

• AlphaFold DB: A0A4R5GBR1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	78.451	100	0.785
pilF	Thermus thermophilus HB27	56.823	100	0.57