Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CWC46_RS06960 Genome accession   NZ_CP025085
Coordinates   1513203..1514195 (+) Length   330 a.a.
NCBI ID   WP_021017593.1    Uniprot ID   A0A2I5TH68
Organism   Prodigiosinella confusarubida strain LacA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1508203..1519195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWC46_RS06925 (CWC46_06925) - 1508684..1508803 (-) 120 WP_416354395.1 entericidin A/B family lipoprotein -
  CWC46_RS06930 (CWC46_06930) hemW 1508968..1510107 (-) 1140 WP_021017599.1 radical SAM family heme chaperone HemW -
  CWC46_RS06935 (CWC46_06935) rdgB 1510100..1510693 (-) 594 WP_021017598.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CWC46_RS06940 (CWC46_06940) yggU 1510711..1511001 (-) 291 WP_021017597.1 DUF167 family protein YggU -
  CWC46_RS06945 (CWC46_06945) - 1510998..1511552 (-) 555 WP_021017596.1 YggT family protein -
  CWC46_RS06950 (CWC46_06950) proC 1511605..1512426 (-) 822 WP_021017595.1 pyrroline-5-carboxylate reductase -
  CWC46_RS06955 (CWC46_06955) - 1512450..1513163 (-) 714 WP_021017594.1 YggS family pyridoxal phosphate-dependent enzyme -
  CWC46_RS06960 (CWC46_06960) pilT 1513203..1514195 (+) 993 WP_021017593.1 type IV pilus twitching motility protein PilT Machinery gene
  CWC46_RS06965 (CWC46_06965) codA 1514369..1515607 (-) 1239 WP_021017592.1 cytosine deaminase -
  CWC46_RS06970 (CWC46_06970) - 1515604..1516839 (-) 1236 WP_021017591.1 PucR family transcriptional regulator -
  CWC46_RS06975 (CWC46_06975) codB 1516843..1518105 (-) 1263 WP_021017590.1 cytosine permease -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 36427.90 Da        Isoelectric Point: 6.7080

>NTDB_id=258336 CWC46_RS06960 WP_021017593.1 1513203..1514195(+) (pilT) [Prodigiosinella confusarubida strain LacA]
MDVHEWVRCSVKQNASDLHLCSGHPPVQRIDGVLRMDNSVPNLRSDQLECWCERWLEPVYQQQLQQCGQVDCALTLPEGQ
RLRVNLFMQRHGLSIALRLIPASHPSLSALKTPPIVAELAERPDGLVLVTGATGSGKSTTLAAMIDALNQRCSRHIITLE
DPIEFLHTSQRCLIQQREIGRHSESFNAALRAALREDPDVLLLGELRDAETIRLALTAAETGHLVLATLHTRSAVQAVER
LVDVFPADEKAFVRTQLAASLQAVVAQKLMPGVGGGRIALFEILMVTPAVSNLIREGKTHQLPSLLQTGGQLGMQTFEQS
YQQRCREGEL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=258336 CWC46_RS06960 WP_021017593.1 1513203..1514195(+) (pilT) [Prodigiosinella confusarubida strain LacA]
ATGGATGTGCATGAATGGGTGAGGTGTAGTGTAAAACAGAATGCCTCTGATCTGCACCTGTGTAGCGGACATCCGCCGGT
ACAGCGTATTGATGGTGTGCTGAGAATGGACAATAGCGTGCCGAACTTGAGATCAGATCAACTGGAATGTTGGTGTGAAC
GCTGGCTGGAACCCGTTTATCAGCAGCAGTTACAACAGTGCGGGCAGGTTGATTGCGCACTCACCTTGCCTGAGGGGCAG
CGTTTGCGGGTGAATCTGTTTATGCAACGGCACGGCCTTTCCATCGCACTGCGGTTGATTCCTGCCTCCCATCCTTCGCT
TTCCGCGCTGAAAACGCCACCGATTGTCGCGGAGTTGGCTGAGCGTCCAGATGGACTGGTTCTGGTGACTGGCGCCACCG
GCAGTGGCAAGTCCACCACATTGGCGGCGATGATCGATGCGCTGAACCAGCGGTGCTCACGGCATATCATCACACTGGAA
GATCCCATCGAGTTTCTTCATACCAGCCAGCGATGTCTGATTCAGCAGCGTGAAATAGGCCGGCACAGTGAGTCATTTAA
CGCGGCATTGCGGGCAGCACTGCGAGAAGACCCAGATGTGCTGTTACTGGGGGAATTACGGGATGCCGAGACTATCCGGC
TGGCATTGACGGCCGCAGAGACCGGACATCTGGTGCTGGCCACGCTACACACCCGTAGCGCTGTGCAGGCGGTAGAACGA
TTGGTGGATGTGTTTCCCGCCGATGAAAAAGCGTTTGTGCGCACTCAACTGGCAGCGAGTTTACAAGCGGTGGTAGCACA
AAAACTGATGCCGGGAGTCGGAGGCGGGCGCATTGCGCTGTTTGAAATACTGATGGTAACGCCTGCCGTTAGTAATCTGA
TCCGGGAAGGCAAAACCCATCAACTCCCCAGTTTGTTGCAAACCGGTGGGCAATTGGGCATGCAAACCTTTGAGCAAAGC
TATCAGCAGCGTTGCCGGGAAGGCGAATTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I5TH68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.22

99.394

0.509
  pilT Vibrio cholerae strain A1552

51.22

99.394

0.509
  pilT Neisseria meningitidis 8013

50.152

99.697

0.5
  pilT Neisseria gonorrhoeae MS11

49.848

99.697

0.497
  pilT Legionella pneumophila strain ERS1305867

50.784

96.667

0.491
  pilT Legionella pneumophila strain Lp02

50.784

96.667

0.491
  pilT Pseudomonas stutzeri DSM 10701

50.629

96.364

0.488
  pilT Pseudomonas aeruginosa PAK

50.314

96.364

0.485
  pilT Acinetobacter baylyi ADP1

48.78

99.394

0.485
  pilT Acinetobacter nosocomialis M2

48.171

99.394

0.479
  pilT Acinetobacter baumannii strain A118

48.758

97.576

0.476
  pilT Acinetobacter baumannii D1279779

48.758

97.576

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.141

96.667

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.577

100

0.397
  pilU Acinetobacter baylyi ADP1

39.441

97.576

0.385
  pilU Vibrio cholerae strain A1552

38.58

98.182

0.379

Multiple sequence alignment