Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CWR52_RS16340 Genome accession   NZ_CP025034
Coordinates   3375171..3376151 (+) Length   326 a.a.
NCBI ID   WP_104950143.1    Uniprot ID   -
Organism   Enterobacter sp. SGAir0187     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3370171..3381151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWR52_RS16300 (CWR52_16435) - 3370254..3370580 (+) 327 WP_003862421.1 YggL family protein -
  CWR52_RS16305 (CWR52_16440) - 3370637..3371353 (+) 717 WP_104950137.1 DUF2884 domain-containing protein -
  CWR52_RS16310 (CWR52_16445) - 3371437..3371775 (-) 339 WP_104950138.1 endonuclease domain-containing protein -
  CWR52_RS16315 (CWR52_16450) hemW 3371839..3372981 (-) 1143 WP_104950139.1 radical SAM family heme chaperone HemW -
  CWR52_RS16320 (CWR52_16455) - 3372974..3373567 (-) 594 WP_104950140.1 XTP/dITP diphosphatase -
  CWR52_RS16325 (CWR52_16460) yggU 3373571..3373867 (-) 297 WP_045909934.1 DUF167 family protein YggU -
  CWR52_RS16330 (CWR52_16465) - 3373864..3374430 (-) 567 WP_104950141.1 YggT family protein -
  CWR52_RS16335 (CWR52_16470) - 3374452..3375153 (-) 702 WP_104950142.1 YggS family pyridoxal phosphate-dependent enzyme -
  CWR52_RS16340 (CWR52_16475) pilT 3375171..3376151 (+) 981 WP_104950143.1 type IV pilus twitching motility protein PilT Machinery gene
  CWR52_RS16345 (CWR52_16480) ruvX 3376162..3376578 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  CWR52_RS16350 (CWR52_16485) - 3376578..3377138 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  CWR52_RS16355 (CWR52_16490) gshB 3377226..3378173 (-) 948 WP_104950144.1 glutathione synthase -
  CWR52_RS16360 (CWR52_16495) rsmE 3378193..3378924 (-) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CWR52_RS16365 (CWR52_16500) endA 3378977..3379684 (-) 708 WP_021242074.1 deoxyribonuclease I -
  CWR52_RS16370 (CWR52_16505) - 3379779..3380276 (-) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35553.53 Da        Isoelectric Point: 6.6964

>NTDB_id=257523 CWR52_RS16340 WP_104950143.1 3375171..3376151(+) (pilT) [Enterobacter sp. SGAir0187]
MDVEEIVTLSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWANGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=257523 CWR52_RS16340 WP_104950143.1 3375171..3376151(+) (pilT) [Enterobacter sp. SGAir0187]
ATGGATGTGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGACTTGAGCCTGCTCCCTTTCCGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCGAACGGGCAGGTGGACTTTGCCGCGACGGTGACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGTGTCTCCGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCACTGGG
TGCGCCGAGGGCCATCCCGGAATTGTTAACCAATGAGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGTAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCACACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAA
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATTGGTCAGCACAGCCCGTCATTTGCCGACGCGCTGCG
CAGCGCCTTACGCCAGGATCCTGACGTGATTTTGCTCGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACCGCAGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAGACCGGTCAGCAGGCAGGAATGCAGAACTTTGATCAGAGCCTGGCCGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

98.773

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment