Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM349_RS12755 Genome accession   NZ_CP024881
Coordinates   2580147..2581127 (-) Length   326 a.a.
NCBI ID   WP_032950062.1    Uniprot ID   A0A9P3X0X4
Organism   Citrobacter freundii strain AR_0022     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2575147..2586127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM349_RS12725 (AM349_12765) - 2575987..2576484 (+) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -
  AM349_RS12730 (AM349_12770) endA 2576579..2577286 (+) 708 WP_016150969.1 deoxyribonuclease I -
  AM349_RS12735 (AM349_12775) rsmE 2577361..2578092 (+) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM349_RS12740 (AM349_12780) gshB 2578112..2579059 (+) 948 WP_003027083.1 glutathione synthase -
  AM349_RS12745 (AM349_12785) - 2579171..2579734 (+) 564 WP_003027086.1 YqgE/AlgH family protein -
  AM349_RS12750 (AM349_12790) ruvX 2579734..2580150 (+) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  AM349_RS12755 (AM349_12795) pilT 2580147..2581127 (-) 981 WP_032950062.1 type IV pilus twitching motility protein PilT Machinery gene
  AM349_RS12760 (AM349_12800) - 2581145..2581849 (+) 705 WP_100280203.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM349_RS12765 (AM349_12805) - 2581868..2582434 (+) 567 WP_003027097.1 YggT family protein -
  AM349_RS12770 (AM349_12810) yggU 2582431..2582721 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  AM349_RS12775 (AM349_12815) - 2582729..2583322 (+) 594 WP_003027104.1 XTP/dITP diphosphatase -
  AM349_RS12780 (AM349_12820) hemW 2583315..2584451 (+) 1137 WP_032950063.1 radical SAM family heme chaperone HemW -
  AM349_RS12785 (AM349_12825) ansB 2584567..2585613 (-) 1047 WP_003027108.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35873.92 Da        Isoelectric Point: 6.3101

>NTDB_id=256105 AM349_RS12755 WP_032950062.1 2580147..2581127(-) (pilT) [Citrobacter freundii strain AR_0022]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKVESAPFTAPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRHCLIQQREVGAHCASFATGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=256105 AM349_RS12755 WP_032950062.1 2580147..2581127(-) (pilT) [Citrobacter freundii strain AR_0022]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGACATGGAAAAGTCGAAAGCGCACCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAACGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCTGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACATTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCACCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGTGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCACAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGATAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTACAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment