Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OA04_RS18445 Genome accession   NZ_CP024842
Coordinates   4033450..4034466 (-) Length   338 a.a.
NCBI ID   WP_107333415.1    Uniprot ID   -
Organism   Pectobacterium versatile strain 3-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4028450..4039466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OA04_RS18430 (OA04_37140) nsrR 4028776..4029201 (-) 426 WP_107333412.1 nitric oxide-sensing transcriptional repressor NsrR -
  OA04_RS18435 (OA04_37150) - 4029545..4031116 (+) 1572 WP_107333413.1 methyl-accepting chemotaxis protein -
  OA04_RS18440 (OA04_37160) - 4031649..4033481 (+) 1833 WP_107333414.1 diguanylate cyclase -
  OA04_RS18445 (OA04_37170) pilT 4033450..4034466 (-) 1017 WP_107333415.1 type IV pilus twitching motility protein PilT Machinery gene
  OA04_RS18450 (OA04_37180) - 4034488..4035201 (+) 714 WP_107333416.1 YggS family pyridoxal phosphate-dependent enzyme -
  OA04_RS18455 (OA04_37190) proC 4035289..4036110 (+) 822 WP_107333738.1 pyrroline-5-carboxylate reductase -
  OA04_RS18460 (OA04_37200) - 4036160..4036714 (+) 555 WP_010295003.1 YggT family protein -
  OA04_RS18465 (OA04_37210) yggU 4036711..4037001 (+) 291 WP_103942383.1 DUF167 family protein YggU -
  OA04_RS18470 (OA04_37220) - 4037033..4037626 (+) 594 WP_107333417.1 XTP/dITP diphosphatase -
  OA04_RS18475 (OA04_37230) hemW 4037619..4038761 (+) 1143 WP_107333418.1 radical SAM family heme chaperone HemW -
  OA04_RS18480 (OA04_37240) - 4038986..4039249 (+) 264 Protein_3550 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36806.05 Da        Isoelectric Point: 6.7126

>NTDB_id=255778 OA04_RS18445 WP_107333415.1 4033450..4034466(-) (pilT) [Pectobacterium versatile strain 3-2]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSAQPSLDALHAPPVFSTLLEKPNGLLLITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFSQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=255778 OA04_RS18445 WP_107333415.1 4033450..4034466(-) (pilT) [Pectobacterium versatile strain 3-2]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCAGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGATGGGGCATTAATGCTGCCTGATGGGCAA
CGCCTGCGGGTTAACGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGCGTTATTCCATCCGCTCAGCCTTCATT
GGACGCGCTACACGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGCTCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACAACGCTGGCGGCCATGCTTGGGGCGTTAAACGACCGCTGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTTC
GCAGGCATTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTACGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCTGGTGAAGAAAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AAAGCTGTTGCCTGCTGCGCAAGGGGGGCGAATCGCGCTTTATGAAGTACTGACCGCCACCGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.849

97.929

0.488
  pilT Pseudomonas aeruginosa PAK

49.547

97.929

0.485
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment