Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   SDE_RS04555 Genome accession   NC_007912
Coordinates   1121089..1121508 (+) Length   139 a.a.
NCBI ID   WP_011467347.1    Uniprot ID   -
Organism   Saccharophagus degradans 2-40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1116089..1126508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SDE_RS04535 (Sde_0860) coaE 1116278..1116889 (-) 612 WP_011467343.1 dephospho-CoA kinase -
  SDE_RS04540 (Sde_0861) pilD 1116889..1117785 (-) 897 WP_011467344.1 prepilin peptidase Machinery gene
  SDE_RS04545 (Sde_0862) pilC 1117788..1119005 (-) 1218 WP_011467345.1 type II secretion system F family protein Machinery gene
  SDE_RS04550 (Sde_0863) pilB 1119009..1120730 (-) 1722 WP_011467346.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SDE_RS04555 (Sde_0864) pilA2 1121089..1121508 (+) 420 WP_011467347.1 pilin Machinery gene
  SDE_RS04560 (Sde_0865) - 1121579..1123504 (+) 1926 WP_041324223.1 hypothetical protein -
  SDE_RS04565 (Sde_0866) - 1123481..1124425 (-) 945 WP_158303854.1 lysylphosphatidylglycerol synthase domain-containing protein -
  SDE_RS04570 (Sde_0867) - 1124331..1124870 (-) 540 WP_011467350.1 acyltransferase -
  SDE_RS04575 (Sde_0868) - 1124909..1125865 (-) 957 WP_011467351.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14366.58 Da        Isoelectric Point: 9.6269

>NTDB_id=25565 SDE_RS04555 WP_011467347.1 1121089..1121508(+) (pilA2) [Saccharophagus degradans 2-40]
MKKVQQGFTLIELMIVVAIIGILAAIALPAYNDYTTRAKVSEGIGLAAAAKSAVSEYRISLAAYPTNNTLAGLPTQIASK
YVSSVNVGAGGIITVGYKAAAGVTAASTIIFRPTFATGTVDWTCTGGTVQDSYRPSNCR

Nucleotide


Download         Length: 420 bp        

>NTDB_id=25565 SDE_RS04555 WP_011467347.1 1121089..1121508(+) (pilA2) [Saccharophagus degradans 2-40]
ATGAAGAAAGTTCAACAAGGTTTTACTCTAATCGAATTAATGATCGTGGTAGCGATTATCGGTATTTTGGCTGCAATCGC
GTTACCTGCGTATAACGATTACACCACTCGTGCAAAAGTTTCTGAAGGTATTGGTTTGGCTGCTGCAGCTAAGTCTGCGG
TGAGTGAGTATCGTATTTCTTTGGCTGCATACCCCACAAACAACACTCTTGCTGGTCTTCCTACTCAGATTGCTAGTAAA
TATGTATCGAGTGTGAATGTTGGAGCTGGAGGTATAATTACTGTGGGTTATAAGGCTGCTGCAGGTGTTACAGCTGCTTC
AACTATTATATTTCGACCTACTTTTGCTACTGGAACTGTAGATTGGACATGTACTGGTGGTACTGTTCAGGATAGCTATC
GCCCTTCCAACTGCCGTTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

57.353

97.842

0.561
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.489

100

0.561
  pilA2 Legionella pneumophila str. Paris

56.618

97.842

0.554
  pilA Ralstonia pseudosolanacearum GMI1000

43.293

100

0.511
  pilE Neisseria gonorrhoeae strain FA1090

43.125

100

0.496
  pilE Neisseria gonorrhoeae MS11

42.236

100

0.489
  comP Acinetobacter baylyi ADP1

43.624

100

0.468
  pilA Haemophilus influenzae 86-028NP

39.456

100

0.417
  pilA/pilA1 Eikenella corrodens VA1

37.662

100

0.417
  pilA Haemophilus influenzae Rd KW20

37.931

100

0.396
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.007

100

0.396
  pilA Glaesserella parasuis strain SC1401

36.184

100

0.396
  pilA Vibrio parahaemolyticus RIMD 2210633

41.085

92.806

0.381
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.899

100

0.374
  pilA Vibrio cholerae C6706

34.899

100

0.374
  pilA Vibrio cholerae strain A1552

34.899

100

0.374

Multiple sequence alignment