Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CTV95_RS19315 Genome accession   NZ_CP024780
Coordinates   4254721..4255737 (+) Length   338 a.a.
NCBI ID   WP_100018224.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain BZA12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4249721..4260737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTV95_RS19285 (CTV95_19285) hemW 4250435..4251577 (-) 1143 WP_100018222.1 radical SAM family heme chaperone HemW -
  CTV95_RS19290 (CTV95_19290) - 4251570..4252163 (-) 594 WP_014916538.1 XTP/dITP diphosphatase -
  CTV95_RS19295 (CTV95_19295) yggU 4252181..4252471 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  CTV95_RS19300 (CTV95_19300) - 4252468..4253022 (-) 555 WP_010295003.1 YggT family protein -
  CTV95_RS19305 (CTV95_19305) proC 4253071..4253892 (-) 822 WP_100018556.1 pyrroline-5-carboxylate reductase -
  CTV95_RS19310 (CTV95_19310) - 4253987..4254700 (-) 714 WP_100018223.1 YggS family pyridoxal phosphate-dependent enzyme -
  CTV95_RS19315 (CTV95_19315) pilT 4254721..4255737 (+) 1017 WP_100018224.1 type IV pilus twitching motility protein PilT Machinery gene
  CTV95_RS19320 (CTV95_19320) - 4255706..4257538 (-) 1833 WP_100018225.1 diguanylate cyclase -
  CTV95_RS19325 (CTV95_19325) - 4258065..4259636 (-) 1572 WP_100018226.1 methyl-accepting chemotaxis protein -
  CTV95_RS19330 (CTV95_19330) nsrR 4259980..4260405 (+) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36678.88 Da        Isoelectric Point: 6.4955

>NTDB_id=255405 CTV95_RS19315 WP_100018224.1 4254721..4255737(+) (pilT) [Pectobacterium brasiliense strain BZA12]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLAALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAEGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=255405 CTV95_RS19315 WP_100018224.1 4254721..4255737(+) (pilT) [Pectobacterium brasiliense strain BZA12]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTTCGACAGGCCGGTCAAGTGGACGGGGCATTAATGCTGCCTGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGTGAAGGGCTGTCTGCGGCGCTACGTATTATCCCGTCCGTTCAACCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGTTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGTGCGTCCTTTGC
GCAGGCGCTACGTGCTGCATTGCGGGAGGATCCCGATGTTATTCTGCTGGGCGAGTTGCGCGATACGGAGACGATTCGAC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGTTGCCCGCTGCGGAAGGGGGCCGAATCGCGCTTTATGAAGTGCTGACGGCGACGGCGGCTGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTGCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment