Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CUC50_RS16420 Genome accession   NZ_CP024675
Coordinates   3409930..3410910 (-) Length   326 a.a.
NCBI ID   WP_121529527.1    Uniprot ID   -
Organism   Citrobacter werkmanii strain UMH18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3404930..3415910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUC50_RS16390 (CUC50_16525) - 3405777..3406274 (+) 498 WP_038633587.1 SprT family zinc-dependent metalloprotease -
  CUC50_RS16395 (CUC50_16530) endA 3406369..3407076 (+) 708 WP_121529526.1 deoxyribonuclease I -
  CUC50_RS16400 (CUC50_16535) rsmE 3407151..3407882 (+) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CUC50_RS16405 (CUC50_16540) gshB 3407895..3408842 (+) 948 WP_038633598.1 glutathione synthase -
  CUC50_RS16410 (CUC50_16545) - 3408954..3409517 (+) 564 WP_032940453.1 YqgE/AlgH family protein -
  CUC50_RS16415 (CUC50_16550) ruvX 3409517..3409933 (+) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  CUC50_RS16420 (CUC50_16555) pilT 3409930..3410910 (-) 981 WP_121529527.1 type IV pilus twitching motility protein PilT Machinery gene
  CUC50_RS16425 (CUC50_16560) - 3410928..3411632 (+) 705 WP_087856786.1 YggS family pyridoxal phosphate-dependent enzyme -
  CUC50_RS16430 (CUC50_16565) - 3411651..3412217 (+) 567 WP_121529528.1 YggT family protein -
  CUC50_RS16435 (CUC50_16570) yggU 3412214..3412504 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  CUC50_RS16440 (CUC50_16575) - 3412512..3413105 (+) 594 WP_038633614.1 XTP/dITP diphosphatase -
  CUC50_RS16445 (CUC50_16580) hemW 3413098..3414234 (+) 1137 WP_087856788.1 radical SAM family heme chaperone HemW -
  CUC50_RS16450 (CUC50_16585) ansB 3414351..3415397 (-) 1047 WP_038633619.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35863.15 Da        Isoelectric Point: 6.8183

>NTDB_id=254934 CUC50_RS16420 WP_121529527.1 3409930..3410910(-) (pilT) [Citrobacter werkmanii strain UMH18]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLANSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALAELLHSENGLILVTGATGSGKSTTLAAMVSYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGIMQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=254934 CUC50_RS16420 WP_121529527.1 3409930..3410910(-) (pilT) [Citrobacter werkmanii strain UMH18]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGGCGAACTCACGGCGTCTGCGCGCG
AGCGCATTTGTGCATCAGCAGGGGATCTCTCTGGCGCTAAGGCTGTTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTAGCTGAGCTACTGCACAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGTCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTAGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCTGATGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGGGGGGCGGCTCAGGCTATTGCGCGGCTGGTGGATACT
TTTCCTGCACCTGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTTGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCATAATGCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAAAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(65-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

98.466

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

38.769

99.693

0.387
  pilU Vibrio cholerae strain A1552

40.323

95.092

0.383

Multiple sequence alignment