Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ADEH_RS03195 Genome accession   NC_007760
Coordinates   716963..718063 (+) Length   366 a.a.
NCBI ID   WP_011419682.1    Uniprot ID   Q2INL9
Organism   Anaeromyxobacter dehalogenans 2CP-C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 711963..723063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ADEH_RS03170 (Adeh_0618) - 712364..713212 (+) 849 WP_011419677.1 purine-nucleoside phosphorylase -
  ADEH_RS03175 (Adeh_0619) - 713259..713612 (+) 354 WP_011419678.1 PilZ domain-containing protein -
  ADEH_RS03180 (Adeh_0620) - 713617..714021 (+) 405 WP_011419679.1 cytidine deaminase -
  ADEH_RS03185 (Adeh_0621) ribF 714097..715068 (+) 972 WP_011419680.1 riboflavin biosynthesis protein RibF -
  ADEH_RS03190 (Adeh_0622) pilB 715243..716949 (+) 1707 WP_011419681.1 type IV-A pilus assembly ATPase PilB -
  ADEH_RS03195 (Adeh_0623) pilT 716963..718063 (+) 1101 WP_011419682.1 type IV pilus twitching motility protein PilT Machinery gene
  ADEH_RS03200 (Adeh_0624) - 718132..719385 (+) 1254 WP_011419683.1 type II secretion system F family protein -
  ADEH_RS03205 (Adeh_0625) - 719399..720979 (+) 1581 WP_011419684.1 two-component system sensor histidine kinase NtrB -
  ADEH_RS03210 (Adeh_0626) - 720992..722374 (+) 1383 WP_011419685.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40134.52 Da        Isoelectric Point: 9.2985

>NTDB_id=25295 ADEH_RS03195 WP_011419682.1 716963..718063(+) (pilT) [Anaeromyxobacter dehalogenans 2CP-C]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLS
RFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELARKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIE
DPIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQAKFGMQTFN
QSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGPPQGAAR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=25295 ADEH_RS03195 WP_011419682.1 716963..718063(+) (pilT) [Anaeromyxobacter dehalogenans 2CP-C]
TTGGCGAACCTGCACCAGCTCCTGAAGGCCATGGTCGAGAAGGGGGCCTCCGACCTCCACGTCACCACCGGCTCCCCGCC
CCAGCTCCGCATCGACGGCAAGCTGGTGCCGCTCAAGACCCCGCCGCTCTCGCCGGTCGAGACGAAGCAGCTCTGCTACT
CGATCCTGACCGACGCGCAGAAGCACAAGTTCGAGGAGGAGAGCGAGCTCGACCTCTCCTTCGGCGTGAAGGGGCTCTCC
CGCTTCCGCGCCAACGTCTTCATGCAGCGCGGCGCGGTCGCCGGCGCGTTCCGCACCATCCCGTTCAAGATCCTCACCTT
CCAGGAGCTGGGCCTGCCGCCCATCGTGGCCGAGCTGGCCAGGAAGCCGCGCGGCCTGGTGCTCGTCACCGGCCCCACCG
GCTCCGGCAAGTCCACCACGCTCGCGTCGATCATCGACAAGATCAACACCGACCGGCACGAGCACATCATCACGATCGAG
GATCCGATCGAGTACCTGCACCCGCACAAGAACTGCGTGGTGAACCAGCGCGAGGTCGGGGCGGACACCGCCTCGTTCAA
GAAGGCGCTCAAGTACATCCTCCGCCAGGATCCCGACGTCGTGCTGGTCGGCGAGATGCGCGACCTCGAGACCATCGAGG
CGGCGCTGGTGATCTCCGAGACCGGCCACCTCGCGTTCGCGACGCTGCACACGAACAGCGCGGTCCAGACCATCAACCGC
ATCCTCGACGTCTTCCCGCCCTACCAGCAGCCGCAGGTCCGCGCGCAGCTCTCCTTCGTGCTGGAGGGCGTGCTCACCCA
GAACCTGCTGCCCAGGGCCGGCGGCCCCGGCCGCGTGCTCATCATCGAGGTGATGGTCCCGAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTGCACCAGGTCTACTCGCAGATGCAGGTCGGCCAGGCCAAGTTCGGCATGCAGACCTTCAAC
CAGAGCCTCGCGATGGCGTTCGCGAAGCGCCTCATCACGCTGGAGGAGGCGCTCGGCCGCTCCTCGGACGCCGAGGAGCT
GAAGAACATCATCGCGAGCGGCGCCGCGCTGCGCGGCCCGCCGCAAGGTGCGGCGCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q2INL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.26

96.721

0.505
  pilT Legionella pneumophila strain Lp02

52.77

93.716

0.495
  pilT Legionella pneumophila strain ERS1305867

52.77

93.716

0.495
  pilT Acinetobacter baylyi ADP1

52.239

91.53

0.478
  pilT Pseudomonas aeruginosa PAK

51.642

91.53

0.473
  pilT Acinetobacter baumannii D1279779

51.045

91.53

0.467
  pilT Acinetobacter baumannii strain A118

51.045

91.53

0.467
  pilT Acinetobacter nosocomialis M2

50.746

91.53

0.464
  pilT Pseudomonas stutzeri DSM 10701

50.746

91.53

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.701

91.257

0.454
  pilT Vibrio cholerae strain A1552

49.701

91.257

0.454
  pilT Neisseria gonorrhoeae MS11

46.921

93.169

0.437
  pilT Neisseria meningitidis 8013

46.921

93.169

0.437
  pilU Pseudomonas stutzeri DSM 10701

42.135

97.268

0.41
  pilU Vibrio cholerae strain A1552

41.16

98.907

0.407
  pilU Acinetobacter baylyi ADP1

39.481

94.809

0.374

Multiple sequence alignment