Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CRN80_RS10800 Genome accession   NZ_CP023969
Coordinates   2295447..2296481 (+) Length   344 a.a.
NCBI ID   WP_098950124.1    Uniprot ID   -
Organism   Pseudomonas sp. FDAARGOS_380     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2290447..2301481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN80_RS10770 (CRN80_10770) - 2290794..2291228 (-) 435 WP_032894709.1 DUF4426 domain-containing protein -
  CRN80_RS10775 (CRN80_10775) metW 2291250..2291870 (-) 621 WP_003195091.1 methionine biosynthesis protein MetW -
  CRN80_RS10780 (CRN80_10780) - 2291878..2293017 (-) 1140 WP_032894712.1 homoserine O-acetyltransferase -
  CRN80_RS10785 (CRN80_10785) - 2293238..2293825 (-) 588 WP_003195087.1 YggT family protein -
  CRN80_RS10790 (CRN80_10790) proC 2293851..2294669 (-) 819 WP_056783041.1 pyrroline-5-carboxylate reductase -
  CRN80_RS10795 (CRN80_10795) - 2294703..2295389 (-) 687 WP_032894716.1 YggS family pyridoxal phosphate-dependent enzyme -
  CRN80_RS10800 (CRN80_10800) pilT 2295447..2296481 (+) 1035 WP_098950124.1 type IV pilus twitching motility protein PilT Machinery gene
  CRN80_RS10805 (CRN80_10805) - 2296622..2297260 (-) 639 WP_056783034.1 C40 family peptidase -
  CRN80_RS10810 (CRN80_10810) - 2297510..2297938 (+) 429 WP_032894720.1 TM2 domain-containing protein -
  CRN80_RS10815 (CRN80_10815) - 2298071..2299342 (-) 1272 WP_098950125.1 dihydroorotase -
  CRN80_RS10820 (CRN80_10820) - 2299339..2300343 (-) 1005 WP_014720325.1 aspartate carbamoyltransferase catalytic subunit -
  CRN80_RS10825 (CRN80_10825) pyrR 2300368..2300874 (-) 507 WP_056783033.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  CRN80_RS10830 (CRN80_10830) ruvX 2301023..2301460 (-) 438 WP_003195067.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37226.00 Da        Isoelectric Point: 7.6886

>NTDB_id=251009 CRN80_RS10800 WP_098950124.1 2295447..2296481(+) (pilT) [Pseudomonas sp. FDAARGOS_380]
MDITQLLTASVSRGASDLHLSAGLAPMLRIDGEVWPLDGPALNATQVVDLLSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRAIATEVQSLEALGLGEVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDDLNRHRRQHILTLED
PIEFIHRPKMALVNQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGVISREEAREKARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=251009 CRN80_RS10800 WP_098950124.1 2295447..2296481(+) (pilT) [Pseudomonas sp. FDAARGOS_380]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCAGACCTGCATCTGTCAGCCGGCCTGGCACCGAT
GCTGCGCATTGATGGCGAGGTTTGGCCCCTGGATGGGCCGGCGCTGAACGCTACCCAGGTGGTCGACTTATTGAGCCCTT
TGCTGAATAAACATCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCGGGTGTGGCCCGC
TTCCGGGCGAACGTATTTCGCCAGGATCGCGGCGTGGGTGCGGTGTTCCGGGCTATTGCGACTGAGGTCCAGAGCCTGGA
AGCGTTGGGATTGGGCGAGGTGTTCCAGCGAATCGCCCGCCTTCCTCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CCGGCAAATCCACGACCCTGGCGGCGATGATCGACGACCTCAACCGGCATCGACGCCAGCATATCCTCACGCTGGAAGAC
CCCATCGAGTTTATCCACAGGCCGAAAATGGCCCTGGTCAACCAGCGCCAGGTGCATCGCGACACCCACAGTTTCTCGGT
GGCCCTGCGTTCAGCCCTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAACTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCAGAGACCGGGCACCTGGTGTTTGGCACCTTGCACACCTCGTCGGCGGCAAAGACGGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAGAAGGCGATGGTCCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGGTGTCTCAGGT
GCTGGTGAAGAAGGTGGGCGGCGGGCGCGTGGCCGCCCATGAAATCATGCTGGGCACCCCGGCGATTCGTAACTTGATTC
GCGAAGACAAGGTGGCGCAGATGGTTTCGGCGATTCAGACCGGTGGGGCGTTGGGGATGAAGACTCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGAGTGATTAGCCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCTGGGGACATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.021

99.128

0.724
  pilT Pseudomonas stutzeri DSM 10701

71.554

99.128

0.709
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

68.035

99.128

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.985

97.965

0.637
  pilT Vibrio cholerae strain A1552

64.985

97.965

0.637
  pilT Legionella pneumophila strain Lp02

63.314

98.256

0.622
  pilT Legionella pneumophila strain ERS1305867

63.314

98.256

0.622
  pilT Neisseria meningitidis 8013

60.882

98.837

0.602
  pilT Neisseria gonorrhoeae MS11

60.588

98.837

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.708

99.419

0.494
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381

Multiple sequence alignment