Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SDY_RS15360 Genome accession   NC_007606
Coordinates   2894588..2895568 (+) Length   326 a.a.
NCBI ID   WP_078164563.1    Uniprot ID   -
Organism   Shigella dysenteriae Sd197     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2889588..2900568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SDY_RS15330 (SDY_3116) - 2890106..2891113 (+) 1008 WP_000737723.1 DUF1202 family protein -
  SDY_RS15335 (SDY_3117) hemW 2891265..2892401 (-) 1137 WP_000239935.1 radical SAM family heme chaperone HemW -
  SDY_RS15340 (SDY_3118) - 2892394..2892987 (-) 594 WP_001174741.1 XTP/dITP diphosphatase -
  SDY_RS15345 (SDY_3119) yggU 2892995..2893285 (-) 291 WP_001277216.1 DUF167 family protein YggU -
  SDY_RS15350 (SDY_3120) yggT 2893282..2893848 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  SDY_RS15355 (SDY_3122) yggS 2893866..2894570 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  SDY_RS15360 (SDY_3121) pilT 2894588..2895568 (+) 981 WP_078164563.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SDY_RS15365 (SDY_3123) ruvX 2895757..2896173 (-) 417 WP_000017103.1 Holliday junction resolvase RuvX -
  SDY_RS15370 (SDY_3124) - 2896173..2896736 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  SDY_RS15375 (SDY_3125) gshB 2896845..2897795 (-) 951 WP_000593287.1 glutathione synthase -
  SDY_RS15380 (SDY_3126) rsmE 2897808..2898539 (-) 732 WP_005018319.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SDY_RS15385 (SDY_3127) endA 2898619..2899326 (-) 708 WP_000286506.1 deoxyribonuclease I -
  SDY_RS15390 (SDY_3128) - 2899421..2899918 (-) 498 WP_000858391.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35838.06 Da        Isoelectric Point: 6.1242

>NTDB_id=25078 SDY_RS15360 WP_078164563.1 2894588..2895568(+) (pilT) [Shigella dysenteriae Sd197]
MNMEEIVALSVKHNVSDLHLCSARPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLADPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VAEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=25078 SDY_RS15360 WP_078164563.1 2894588..2895568(+) (pilT) [Shigella dysenteriae Sd197]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCAGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCACCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAACTTGG
TGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGCAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGCGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365

Multiple sequence alignment