Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AT852_RS03185 Genome accession   NZ_CP023645
Coordinates   557921..558901 (+) Length   326 a.a.
NCBI ID   WP_014334060.1    Uniprot ID   -
Organism   Shigella sonnei strain CFSAN030807     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552921..563901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT852_RS03150 (AT852_03150) - 553068..553256 (-) 189 Protein_561 TRAP transporter small permease subunit -
  AT852_RS03155 (AT852_03155) - 553301..554287 (-) 987 Protein_562 TRAP transporter substrate-binding protein -
  AT852_RS03160 (AT852_03160) hemW 554598..555734 (-) 1137 WP_119180258.1 radical SAM family heme chaperone HemW -
  AT852_RS03165 (AT852_03165) - 555727..556320 (-) 594 WP_001174756.1 XTP/dITP diphosphatase -
  AT852_RS03170 (AT852_03170) yggU 556328..556618 (-) 291 WP_001277227.1 DUF167 family protein YggU -
  AT852_RS03175 (AT852_03175) yggT 556615..557181 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  AT852_RS03180 (AT852_03180) yggS 557199..557903 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  AT852_RS03185 (AT852_03185) pilT 557921..558901 (+) 981 WP_014334060.1 type IV pilus twitching motility protein PilT Machinery gene
  AT852_RS03190 (AT852_03190) ruvX 559076..559492 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  AT852_RS03195 (AT852_03195) - 559492..560055 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  AT852_RS03200 (AT852_03200) gshB 560164..561114 (-) 951 WP_000593279.1 glutathione synthase -
  AT852_RS03205 (AT852_03205) rsmE 561127..561858 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AT852_RS03210 (AT852_03210) endA 561938..562645 (-) 708 WP_000286505.1 deoxyribonuclease I -
  AT852_RS03215 (AT852_03215) - 562740..563237 (-) 498 WP_005137173.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35940.19 Da        Isoelectric Point: 5.9558

>NTDB_id=248872 AT852_RS03185 WP_014334060.1 557921..558901(+) (pilT) [Shigella sonnei strain CFSAN030807]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=248872 AT852_RS03185 WP_014334060.1 557921..558901(+) (pilT) [Shigella sonnei strain CFSAN030807]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGAGGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTTGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGTGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCAGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGAAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.906

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362

Multiple sequence alignment