Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCAR_RS03215 Genome accession   NC_007498
Coordinates   615098..616141 (-) Length   347 a.a.
NCBI ID   WP_011340194.1    Uniprot ID   Q3A779
Organism   Syntrophotalea carbinolica DSM 2380     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 610098..621141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCAR_RS03195 (Pcar_0501) - 610640..612151 (+) 1512 WP_011340189.1 amidase -
  PCAR_RS03200 (Pcar_0502) - 612820..614112 (+) 1293 WP_011340191.1 type II toxin-antitoxin system HipA family toxin -
  PCAR_RS03205 (Pcar_0503) - 614129..614404 (+) 276 WP_011340192.1 helix-turn-helix domain-containing protein -
  PCAR_RS03210 (Pcar_0504) - 614633..615082 (-) 450 WP_011340193.1 hypothetical protein -
  PCAR_RS03215 (Pcar_0505) pilT 615098..616141 (-) 1044 WP_011340194.1 type IV pilus twitching motility protein PilT Machinery gene
  PCAR_RS03220 (Pcar_0506) - 616640..617020 (-) 381 WP_011340195.1 VOC family protein -
  PCAR_RS03225 (Pcar_0507) cls 617402..618841 (+) 1440 WP_011340196.1 cardiolipin synthase -
  PCAR_RS03230 (Pcar_0508) - 619011..619418 (-) 408 WP_011340197.1 bacteriohemerythrin -
  PCAR_RS03235 (Pcar_0509) - 619574..620119 (-) 546 WP_011340198.1 nitroreductase family protein -
  PCAR_RS03240 (Pcar_0510) - 620152..620445 (-) 294 WP_011340199.1 DnaJ domain-containing protein -
  PCAR_RS03245 (Pcar_0511) - 620725..621135 (+) 411 WP_011340200.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38247.17 Da        Isoelectric Point: 8.8537

>NTDB_id=24857 PCAR_RS03215 WP_011340194.1 615098..616141(-) (pilT) [Syntrophotalea carbinolica DSM 2380]
MAKIDALFKILKEKGGSDLHLSAGNPPLIRRSGQLEPILTQVLTHEQNKALLYEIMGEAQRKTYETTRDLDFAYEVTELK
SRFRANIFMGRLGMGAVFRLIPAEILSADQLGLPPAVLDFTQFKKGLVLVTGPTGSGKSTTLAAMIDHVNRTRKEHILTV
EDPIEFVHVSQQSLINQREVGRHTRSFAAALKAALREDPDLILVGEMRDLETIELAITAAETGHLVFGTLHTSSAAKTVD
RIINVFPTNQQEQVRTMLGESLKGVIAQQLLRTVDGKRCAALEILSVNSAVSNLIREGKTFQIPSVIQTGKTQGMQLMDQ
AIQDLLTAGKITREEAHKFASNKALFQ

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=24857 PCAR_RS03215 WP_011340194.1 615098..616141(-) (pilT) [Syntrophotalea carbinolica DSM 2380]
ATGGCAAAAATTGATGCGCTGTTCAAAATATTGAAAGAAAAAGGCGGATCGGACCTGCACCTTTCAGCAGGCAATCCGCC
GCTTATACGCCGATCGGGGCAACTGGAACCCATTTTGACTCAAGTTCTGACCCACGAACAAAATAAGGCGTTGCTCTACG
AAATTATGGGCGAAGCCCAGCGCAAAACCTATGAAACGACCCGCGATCTCGATTTTGCCTATGAGGTAACGGAGCTTAAA
TCGCGTTTTCGTGCCAACATCTTTATGGGCCGGCTCGGTATGGGCGCTGTGTTTCGCCTGATTCCCGCTGAGATCCTCAG
CGCCGACCAGCTTGGCCTTCCCCCTGCGGTACTCGATTTCACCCAGTTCAAAAAAGGTCTGGTACTGGTCACCGGACCGA
CCGGAAGCGGCAAATCGACCACCCTGGCGGCCATGATCGATCATGTCAACCGGACCCGCAAAGAACACATCCTTACCGTC
GAAGATCCCATTGAATTCGTGCATGTCAGCCAGCAGAGCCTCATCAATCAGCGAGAAGTCGGGCGCCACACCCGCTCCTT
TGCCGCCGCCCTCAAAGCGGCTCTACGCGAAGATCCGGATCTGATCCTGGTCGGCGAGATGCGCGATCTGGAAACCATCG
AACTGGCCATCACGGCGGCAGAAACCGGGCATCTGGTATTTGGCACCCTGCATACCAGCAGCGCCGCCAAAACGGTGGAT
CGCATCATCAATGTCTTTCCCACCAACCAGCAGGAGCAGGTCCGCACCATGCTCGGCGAATCCCTCAAGGGAGTCATCGC
CCAACAACTGCTAAGGACGGTGGACGGCAAGCGTTGCGCCGCCCTTGAGATCCTGTCTGTAAATTCGGCCGTCTCCAACC
TGATCCGGGAAGGTAAAACCTTCCAGATTCCGTCGGTTATTCAAACCGGTAAAACGCAGGGCATGCAGCTCATGGACCAG
GCCATTCAGGATCTTCTGACCGCGGGAAAAATCACCCGCGAGGAAGCTCACAAATTCGCCTCCAACAAGGCCCTGTTCCA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3A779

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

57.704

95.389

0.55
  pilT Legionella pneumophila strain ERS1305867

57.704

95.389

0.55
  pilT Neisseria meningitidis 8013

54.678

98.559

0.539
  pilT Acinetobacter baylyi ADP1

56.495

95.389

0.539
  pilT Pseudomonas stutzeri DSM 10701

53.936

98.847

0.533
  pilT Neisseria gonorrhoeae MS11

54.252

98.271

0.533
  pilT Pseudomonas aeruginosa PAK

54.303

97.118

0.527
  pilT Acinetobacter baumannii strain A118

54.79

96.254

0.527
  pilT Acinetobacter baumannii D1279779

54.79

96.254

0.527
  pilT Acinetobacter nosocomialis M2

54.79

96.254

0.527
  pilT Vibrio cholerae strain A1552

53.916

95.677

0.516
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.916

95.677

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.395

96.254

0.504
  pilU Pseudomonas stutzeri DSM 10701

40.525

98.847

0.401
  pilU Vibrio cholerae strain A1552

42.857

92.795

0.398
  pilU Acinetobacter baylyi ADP1

39.763

97.118

0.386

Multiple sequence alignment