Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PFL01_RS26770 Genome accession   NC_007492
Coordinates   5982168..5983202 (-) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas fluorescens Pf0-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5977168..5988202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL01_RS26740 (Pfl01_5312) ruvX 5977462..5977899 (+) 438 WP_007953334.1 Holliday junction resolvase RuvX -
  PFL01_RS26745 (Pfl01_5313) pyrR 5977981..5978484 (+) 504 WP_011336369.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PFL01_RS26750 (Pfl01_5314) - 5978512..5979516 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  PFL01_RS26755 (Pfl01_5315) - 5979513..5980784 (+) 1272 WP_011336370.1 dihydroorotase -
  PFL01_RS26760 (Pfl01_5316) - 5980821..5981255 (-) 435 WP_007953343.1 NINE protein -
  PFL01_RS26765 (Pfl01_5317) - 5981505..5982122 (+) 618 WP_011336371.1 C40 family peptidase -
  PFL01_RS26770 (Pfl01_5318) pilT 5982168..5983202 (-) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  PFL01_RS26775 (Pfl01_5319) - 5983259..5983945 (+) 687 WP_011336372.1 YggS family pyridoxal phosphate-dependent enzyme -
  PFL01_RS26780 (Pfl01_5320) proC 5983969..5984787 (+) 819 WP_011336373.1 pyrroline-5-carboxylate reductase -
  PFL01_RS26785 (Pfl01_5321) - 5984798..5985388 (+) 591 WP_011336374.1 YggT family protein -
  PFL01_RS26790 (Pfl01_5322) - 5985385..5985675 (+) 291 WP_011336375.1 DUF167 domain-containing protein -
  PFL01_RS26795 (Pfl01_5323) - 5985741..5986880 (+) 1140 WP_011336376.1 homoserine O-succinyltransferase MetX -
  PFL01_RS26800 (Pfl01_5324) metW 5986888..5987508 (+) 621 WP_011336377.1 methionine biosynthesis protein MetW -
  PFL01_RS26805 (Pfl01_5325) - 5987535..5987969 (+) 435 WP_011336378.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=24850 PFL01_RS26770 WP_007953348.1 5982168..5983202(-) (pilT) [Pseudomonas fluorescens Pf0-1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=24850 PFL01_RS26770 WP_007953348.1 5982168..5983202(-) (pilT) [Pseudomonas fluorescens Pf0-1]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTGGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAACAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGTGGCGCTGGCGCGGTGTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCACGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCAACGACCCTGGCGGCGATGATCGATTACCTCAACACCCATCGCCATCACCACATCCTGACCATTGAAGAT
CCGATCGAATTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGTTTCGCCAC
CGCATTGCGTTCGGCATTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAGATGCGCGATCTGGAAACCATTCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCACCTGGTGTTCGGCACCTTGCACACTACTTCGGCAGCCAAGACCATCGACCGTGTC
GTGGACGTGTTCCCCGGGGACGAAAAATCCATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGTGGTCGGGTGGCGGCGCACGAGATCATGCTGGGCACTTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATACAGACCGGCGGCTCGCTGGGGATGCAGACACTCGACATGTGCCTG
AAGGATCTGGTGACCAAAGGCTTGATCAGCCGCGAACATGCGCGGGAGAAGGCGCGCACGCCGGATAACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment