Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NOC_RS15910 Genome accession   NC_007484
Coordinates   3395949..3396986 (+) Length   345 a.a.
NCBI ID   WP_002813612.1    Uniprot ID   Q3J6V0
Organism   Nitrosococcus oceani ATCC 19707     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3390949..3401986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOC_RS15880 (Noc_2998) pyrE 3391314..3391976 (-) 663 WP_002812459.1 orotate phosphoribosyltransferase -
  NOC_RS15885 (Noc_2999) - 3392133..3393329 (+) 1197 WP_197054485.1 AmpG family muropeptide MFS transporter -
  NOC_RS15890 (Noc_3000) - 3393341..3393649 (-) 309 WP_002812300.1 DUF167 domain-containing protein -
  NOC_RS15895 (Noc_3001) - 3393657..3394220 (-) 564 WP_002813975.1 YggT family protein -
  NOC_RS15900 (Noc_3002) proC 3394213..3395049 (-) 837 WP_011331123.1 pyrroline-5-carboxylate reductase -
  NOC_RS15905 (Noc_3003) - 3395145..3395840 (-) 696 WP_002812025.1 YggS family pyridoxal phosphate-dependent enzyme -
  NOC_RS15910 (Noc_3004) pilT 3395949..3396986 (+) 1038 WP_002813612.1 type IV pilus twitching motility protein PilT Machinery gene
  NOC_RS18300 - 3397526..3397624 (+) 99 Protein_3151 IS5/IS1182 family transposase -
  NOC_RS15915 (Noc_3006) - 3398585..3399256 (+) 672 WP_011331125.1 hypothetical protein -
  NOC_RS15920 (Noc_3007) - 3399563..3399946 (+) 384 WP_002813535.1 hypothetical protein -
  NOC_RS15925 (Noc_3008) hemE 3400559..3401632 (-) 1074 WP_002812603.1 uroporphyrinogen decarboxylase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38284.86 Da        Isoelectric Point: 6.3499

>NTDB_id=24824 NOC_RS15910 WP_002813612.1 3395949..3396986(+) (pilT) [Nitrosococcus oceani ATCC 19707]
MDIAELLAFSVKNSASDLHISAGLPPMIRVDGDVRRINLPPMEHKDVHAMIYDIMNDKQRKDYEEFLETDFSFEIPGLAR
FRVNAFNQNRGAAAVFRTIPSQVLTLEELDAPAVFKNIADNPRGVVLVTGPTGSGKSTTLAAMVNYKNENDFAHILTIED
PIEFVHESKKSLINQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKFGGGRVAAHEIMIGNPAIRNLIREDKIPQMYSAIQTGQEAGMQTLDQCL
SGLLRRNIVTKQEAAKKAVSKELFQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=24824 NOC_RS15910 WP_002813612.1 3395949..3396986(+) (pilT) [Nitrosococcus oceani ATCC 19707]
ATGGATATCGCTGAACTACTTGCCTTTAGTGTCAAGAATTCTGCCTCCGATTTACACATCTCGGCGGGCCTCCCGCCAAT
GATCCGAGTAGACGGCGACGTGCGCCGAATTAACTTACCGCCTATGGAGCACAAGGATGTCCATGCCATGATCTACGATA
TCATGAATGACAAGCAGCGTAAGGATTATGAAGAATTTCTGGAGACCGATTTTTCCTTCGAGATCCCTGGATTAGCCCGT
TTCCGCGTGAATGCGTTCAATCAGAACCGCGGGGCAGCGGCGGTATTCCGCACCATTCCCTCTCAAGTACTCACCCTTGA
AGAACTCGATGCCCCCGCCGTATTTAAGAATATCGCCGATAATCCCCGAGGGGTGGTGTTAGTGACCGGGCCTACCGGCT
CTGGAAAATCAACTACCTTAGCGGCTATGGTAAATTATAAAAATGAGAATGATTTTGCTCATATTTTAACGATCGAGGAT
CCCATCGAATTCGTCCACGAAAGCAAAAAAAGCCTGATTAATCAACGGGAAGTCCATCGGGATACCCATGGCTTTAGCGA
AGCTCTGCGTTCAGCATTGCGGGAAGACCCTGATATTATTCTGGTAGGTGAGCTGCGAGATCTTGAGACTATTCGTCTCG
CATTGACAGCGGCAGAGACGGGGCATTTAGTCTTCGGCACTTTGCATACTAGTTCTGCGGCAAAGACTATTGATCGTATT
ATCGATGTGTTTCCTGCTGCGGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCCGTAATTTCCCAGAC
TCTCCTTAAAAAATTTGGCGGTGGACGGGTTGCTGCCCACGAAATCATGATTGGCAACCCGGCCATTCGTAATCTCATCC
GCGAAGACAAGATCCCACAAATGTATTCTGCTATCCAAACGGGCCAAGAGGCAGGTATGCAGACTCTGGATCAATGTCTG
TCAGGGTTACTTCGTCGCAATATCGTCACTAAGCAGGAGGCAGCTAAGAAAGCCGTAAGCAAAGAGCTTTTCCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3J6V0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

69.388

99.42

0.69
  pilT Neisseria gonorrhoeae MS11

69.096

99.42

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Vibrio cholerae strain A1552

40.762

98.841

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.889

99.13

0.386

Multiple sequence alignment