Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CO701_RS02430 Genome accession   NZ_CP023504
Coordinates   478383..479363 (+) Length   326 a.a.
NCBI ID   WP_096755744.1    Uniprot ID   -
Organism   Citrobacter werkmanii strain FDAARGOS_364     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473383..484363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CO701_RS02400 (CO701_02400) ansB 473888..474934 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  CO701_RS02405 (CO701_02405) hemW 475059..476195 (-) 1137 WP_096755740.1 radical SAM family heme chaperone HemW -
  CO701_RS02410 (CO701_02410) - 476188..476781 (-) 594 WP_096755741.1 XTP/dITP diphosphatase -
  CO701_RS02415 (CO701_02415) yggU 476789..477079 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  CO701_RS02420 (CO701_02420) - 477076..477642 (-) 567 WP_096755742.1 YggT family protein -
  CO701_RS02425 (CO701_02425) - 477661..478365 (-) 705 WP_096755743.1 YggS family pyridoxal phosphate-dependent enzyme -
  CO701_RS02430 (CO701_02430) pilT 478383..479363 (+) 981 WP_096755744.1 type IV pilus twitching motility protein PilT Machinery gene
  CO701_RS02435 (CO701_02435) ruvX 479373..479789 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  CO701_RS02440 (CO701_02440) - 479789..480352 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  CO701_RS02445 (CO701_02445) gshB 480528..481475 (-) 948 WP_096755747.1 glutathione synthase -
  CO701_RS02450 (CO701_02450) rsmE 481488..482219 (-) 732 WP_096755748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CO701_RS02455 (CO701_02455) endA 482294..483001 (-) 708 WP_096755749.1 deoxyribonuclease I -
  CO701_RS02460 (CO701_02460) - 483239..484114 (+) 876 WP_096755750.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35697.79 Da        Isoelectric Point: 6.3361

>NTDB_id=247795 CO701_RS02430 WP_096755744.1 478383..479363(+) (pilT) [Citrobacter werkmanii strain FDAARGOS_364]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVDALLLAWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPVALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
YRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=247795 CO701_RS02430 WP_096755744.1 478383..479363(+) (pilT) [Citrobacter werkmanii strain FDAARGOS_364]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGACGCGCTGCTCCTGGCCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGTTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TACCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAGCTGGCGGGTAGCTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTCGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGCGGGTATGCAAACATTTGCCCAAAGCCTGCAGCAGCGT
CAGGCGCAGGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

37.963

99.387

0.377

Multiple sequence alignment