Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLM72_RS26565 Genome accession   NZ_CP023269
Coordinates   5776237..5777271 (-) Length   344 a.a.
NCBI ID   WP_054897576.1    Uniprot ID   -
Organism   Pseudomonas sp. MYb193     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5771237..5782271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLM72_RS26535 (CLM72_26360) ruvX 5771308..5771745 (+) 438 WP_105228851.1 Holliday junction resolvase RuvX -
  CLM72_RS26540 (CLM72_26365) pyrR 5771878..5772384 (+) 507 WP_019817751.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  CLM72_RS26545 (CLM72_26370) - 5772409..5773413 (+) 1005 WP_034128938.1 aspartate carbamoyltransferase catalytic subunit -
  CLM72_RS26550 (CLM72_26375) - 5773410..5774681 (+) 1272 WP_105228852.1 dihydroorotase -
  CLM72_RS26555 (CLM72_26380) - 5774772..5775200 (-) 429 WP_054897575.1 TM2 domain-containing protein -
  CLM72_RS26560 (CLM72_26385) - 5775450..5776088 (+) 639 WP_034128941.1 C40 family peptidase -
  CLM72_RS26565 (CLM72_26390) pilT 5776237..5777271 (-) 1035 WP_054897576.1 type IV pilus twitching motility protein PilT Machinery gene
  CLM72_RS26570 (CLM72_26395) - 5777329..5778015 (+) 687 WP_105228853.1 YggS family pyridoxal phosphate-dependent enzyme -
  CLM72_RS26575 (CLM72_26400) proC 5778050..5778868 (+) 819 WP_032902278.1 pyrroline-5-carboxylate reductase -
  CLM72_RS26580 (CLM72_26405) - 5778894..5779481 (+) 588 WP_105228854.1 YggT family protein -
  CLM72_RS26590 (CLM72_26410) - 5779704..5780855 (+) 1152 WP_034128945.1 homoserine O-acetyltransferase -
  CLM72_RS26595 (CLM72_26415) metW 5780852..5781472 (+) 621 WP_003195091.1 methionine biosynthesis protein MetW -
  CLM72_RS26600 (CLM72_26420) - 5781494..5781928 (+) 435 WP_105228856.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37266.01 Da        Isoelectric Point: 7.2995

>NTDB_id=245625 CLM72_RS26565 WP_054897576.1 5776237..5777271(-) (pilT) [Pseudomonas sp. MYb193]
MDITQLLTASVSRGASDLHLSAGLAPMLRIDGEVWPLDGPALNATQVVDLLSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRAIATEVQSLEDLGLGEVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDDLNRHRRQHILTLED
PIEFIHRPKLALVNQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGLVSREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=245625 CLM72_RS26565 WP_054897576.1 5776237..5777271(-) (pilT) [Pseudomonas sp. MYb193]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCAGACCTGCATCTGTCAGCCGGCCTGGCACCGAT
GCTGCGCATTGATGGCGAGGTTTGGCCGCTGGATGGGCCAGCGCTGAACGCTACCCAAGTGGTCGACTTATTGAGCCCTT
TGCTGAATAAACATCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAATTGCCAGGCGTCGCCCGG
TTCCGGGCGAACGTGTTCCGCCAGGATCGCGGCGTGGGTGCGGTGTTCCGGGCTATTGCGACGGAGGTCCAAAGCCTGGA
GGATCTGGGACTGGGCGAGGTGTTCCAGCGTATCGCCCGCCTTCCTCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CGGGCAAGTCCACGACCCTGGCGGCGATGATCGACGACCTCAACCGGCATCGACGCCAGCATATCCTCACGCTGGAAGAC
CCCATCGAGTTTATCCACAGGCCGAAACTGGCGCTGGTCAACCAGCGCCAGGTGCATCGCGACACCCACAGTTTCTCGGT
GGCCCTGCGTTCAGCCCTGCGGGAAGATCCGGATGTGATCCTGGTGGGTGAATTGCGCGATCTGGAAACCATACGCCTTG
CGCTGACGGCGGCAGAGACCGGGCACCTGGTGTTCGGCACCCTGCACACCTCATCGGCGGCAAAGACAGTGGACCGGCTG
GTAGACGTCTTCCCGGCCGGGGAGAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCTCAGGT
GCTGGTGAAGAAGGTGGGCGGTGGGCGCGTGGCCGCCCATGAAATCATGCTGGGTACCCCGGCGATTCGTAACTTGATTC
GCGAAGACAAGGTGGCGCAGATGGTTTCGGCTATCCAGACGGGAGGGGCGTTGGGGATGAAGACCCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGGCTGGTCAGTCGGGAAGAGGCGCGGGAGAAGGCGAGGGTACCGGCAGATATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.384

100

0.724
  pilT Pseudomonas stutzeri DSM 10701

71.221

100

0.712
  pilT Acinetobacter baumannii D1279779

68.622

99.128

0.68
  pilT Acinetobacter baumannii strain A118

68.622

99.128

0.68
  pilT Acinetobacter nosocomialis M2

68.622

99.128

0.68
  pilT Acinetobacter baylyi ADP1

67.742

99.128

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.985

97.965

0.637
  pilT Vibrio cholerae strain A1552

64.985

97.965

0.637
  pilT Legionella pneumophila strain Lp02

63.314

98.256

0.622
  pilT Legionella pneumophila strain ERS1305867

63.314

98.256

0.622
  pilT Neisseria meningitidis 8013

60.882

98.837

0.602
  pilT Neisseria gonorrhoeae MS11

60.588

98.837

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381

Multiple sequence alignment