Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLM71_RS19740 Genome accession   NZ_CP023268
Coordinates   4131244..4132236 (-) Length   330 a.a.
NCBI ID   WP_105232506.1    Uniprot ID   -
Organism   Serratia sp. MYb239     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4126244..4137236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLM71_RS19725 (CLM71_19645) - 4126390..4126830 (-) 441 WP_105232503.1 DUF1090 domain-containing protein -
  CLM71_RS19730 (CLM71_19650) - 4126935..4130474 (-) 3540 WP_105232504.1 transporter substrate-binding domain-containing protein -
  CLM71_RS19735 (CLM71_19655) - 4130467..4131099 (-) 633 WP_158684837.1 response regulator transcription factor -
  CLM71_RS19740 (CLM71_19660) pilT 4131244..4132236 (-) 993 WP_105232506.1 type IV pilus twitching motility protein PilT Machinery gene
  CLM71_RS19745 (CLM71_19665) - 4132257..4132964 (+) 708 WP_105232507.1 YggS family pyridoxal phosphate-dependent enzyme -
  CLM71_RS19750 (CLM71_19670) proC 4132985..4133806 (+) 822 WP_105232508.1 pyrroline-5-carboxylate reductase -
  CLM71_RS19755 (CLM71_19675) - 4133826..4134380 (+) 555 WP_015673797.1 YggT family protein -
  CLM71_RS19760 (CLM71_19680) yggU 4134377..4134667 (+) 291 WP_105232509.1 DUF167 family protein YggU -
  CLM71_RS19765 (CLM71_19685) - 4134685..4135281 (+) 597 WP_105232510.1 XTP/dITP diphosphatase -
  CLM71_RS19770 (CLM71_19690) hemW 4135271..4136422 (+) 1152 WP_105232511.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35960.25 Da        Isoelectric Point: 7.2386

>NTDB_id=245599 CLM71_RS19740 WP_105232506.1 4131244..4132236(-) (pilT) [Serratia sp. MYb239]
MDIDEFVALSVKHNASDLHLCTAQPLMMRIDGELQPLAGDAVLPAEDMQRLCAALLDERQLRQLRRTGQLDCALTTTGGT
RLRANFFYQRTGVSLALRRIAERCPTLEQLQAPAIIPALTEREEGLILVTGATGSGKSTTLAAMIDHINRHQRRHILTLE
DPIEFIHHSAQSLIQQRELGRDTRSFDAALSAALREDPDVILVGELRDSASIRLALTAAETGHLVLATLHTRNAPQAVER
VIDVFPAEEKAYVRAQLASGLQAVIAQKLQRRVGGGRTAVFEILTATPAVSSMIREGKTHQLASVLQSGAQAGMQTFAQG
IQQRIAQGIL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=245599 CLM71_RS19740 WP_105232506.1 4131244..4132236(-) (pilT) [Serratia sp. MYb239]
ATGGATATCGATGAATTTGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACCGCCCAACCGTTGAT
GATGCGCATCGATGGTGAACTTCAGCCGCTGGCGGGCGACGCCGTTCTGCCCGCAGAAGACATGCAACGGCTGTGTGCGG
CGCTGCTCGACGAGCGCCAACTGCGGCAGCTGCGACGAACCGGTCAGTTGGATTGTGCCTTGACGACCACGGGCGGGACG
CGGCTGCGCGCCAACTTTTTTTATCAGCGCACCGGCGTGTCGCTGGCGCTGCGCCGCATTGCGGAACGCTGCCCGACGCT
GGAACAGCTGCAGGCGCCGGCGATTATTCCGGCGCTCACCGAGCGGGAAGAGGGGCTGATTCTGGTGACGGGAGCGACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACCATATCAACCGTCATCAGCGGCGGCATATATTGACGCTGGAA
GATCCGATCGAATTTATCCATCACAGCGCGCAGTCGCTGATTCAGCAGCGTGAACTGGGCCGCGATACCCGCAGTTTCGA
CGCGGCGTTAAGCGCGGCGCTGCGCGAAGATCCTGACGTTATTCTGGTGGGTGAACTGCGCGACAGCGCCAGCATCCGGC
TGGCGCTGACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGTTGCACACTCGTAATGCTCCCCAGGCGGTTGAACGG
GTGATCGACGTTTTTCCGGCGGAGGAGAAAGCCTACGTGCGTGCCCAACTGGCCAGCGGTTTACAGGCGGTGATTGCGCA
GAAATTACAGCGCAGGGTCGGCGGCGGCAGAACGGCGGTGTTTGAGATCCTGACGGCGACGCCGGCGGTAAGCAGCATGA
TTCGCGAGGGAAAAACGCATCAGCTGGCCAGCGTATTGCAAAGCGGCGCGCAGGCCGGCATGCAAACCTTTGCTCAGGGC
ATACAGCAACGTATTGCGCAGGGAATTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.727

100

0.527
  pilT Vibrio cholerae strain A1552

52.727

100

0.527
  pilT Acinetobacter baylyi ADP1

49.249

100

0.497
  pilT Acinetobacter baumannii D1279779

48.949

100

0.494
  pilT Acinetobacter baumannii strain A118

48.949

100

0.494
  pilT Acinetobacter nosocomialis M2

48.649

100

0.491
  pilT Pseudomonas stutzeri DSM 10701

47.748

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.605

100

0.482
  pilT Neisseria meningitidis 8013

47.305

100

0.479
  pilT Legionella pneumophila strain ERS1305867

46.547

100

0.47
  pilT Legionella pneumophila strain Lp02

46.547

100

0.47
  pilT Pseudomonas aeruginosa PAK

46.687

100

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.855

96.364

0.452
  pilU Vibrio cholerae strain A1552

40.798

98.788

0.403
  pilU Pseudomonas stutzeri DSM 10701

39.514

99.697

0.394
  pilU Acinetobacter baylyi ADP1

39.319

97.879

0.385

Multiple sequence alignment