Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSYR_RS02470 Genome accession   NC_007005
Coordinates   516463..517497 (+) Length   344 a.a.
NCBI ID   WP_003404551.1    Uniprot ID   A0A0L8INC2
Organism   Pseudomonas syringae pv. syringae B728a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511463..522497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYR_RS02440 (Psyr_0472) rdgB 511604..512197 (-) 594 WP_011266424.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PSYR_RS02445 (Psyr_0473) metW 512231..512851 (-) 621 WP_003316724.1 methionine biosynthesis protein MetW -
  PSYR_RS02450 (Psyr_0474) - 512859..513998 (-) 1140 WP_011266425.1 homoserine O-succinyltransferase MetX -
  PSYR_RS02455 (Psyr_0475) - 514265..514855 (-) 591 WP_003404543.1 YggT family protein -
  PSYR_RS02460 (Psyr_0476) proC 514881..515699 (-) 819 WP_011266426.1 pyrroline-5-carboxylate reductase -
  PSYR_RS02465 (Psyr_0477) - 515721..516407 (-) 687 WP_011266427.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSYR_RS02470 (Psyr_0478) pilT 516463..517497 (+) 1035 WP_003404551.1 type IV pilus twitching motility protein PilT Machinery gene
  PSYR_RS02475 (Psyr_0479) - 517592..518269 (-) 678 WP_011266428.1 C40 family peptidase -
  PSYR_RS02480 (Psyr_0480) - 518520..518924 (+) 405 WP_003404557.1 NINE protein -
  PSYR_RS02490 (Psyr_0481) - 519207..520478 (-) 1272 WP_011266429.1 dihydroorotase -
  PSYR_RS02495 (Psyr_0482) - 520475..521479 (-) 1005 WP_003366142.1 aspartate carbamoyltransferase catalytic subunit -
  PSYR_RS02500 (Psyr_0483) pyrR 521492..522004 (-) 513 WP_011266430.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37976.79 Da        Isoelectric Point: 7.0295

>NTDB_id=24319 PSYR_RS02470 WP_003404551.1 516463..517497(+) (pilT) [Pseudomonas syringae pv. syringae B728a]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=24319 PSYR_RS02470 WP_003404551.1 516463..517497(+) (pilT) [Pseudomonas syringae pv. syringae B728a]
ATGGATATTACCGAGCTGTTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTGCGGCGTATCAACCTGCCCCCGCTGGACGCGAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAACGGCAGGATTTCGAAGAGCGGCTGGAAACCGACTTTTCCTTTGAAGTGCCGGGCGTGGCGCGG
TTTCGGGTCAACGCCTTCAACCAGAACCGCGGTGCTGGCGCGGTATTCCGGACCATTCCCTCGAAAATCCTGAGCATGGA
AGACTTGGGCATGGGCAATGTGTTTCGCAAGATTACCGACGTGGCGCGCGGCCTGATTCTGGTTACCGGGCCGACCGGCT
CAGGCAAGTCGACCACCCTGGCGGCAATGATCGACTACCTCAATAACAATAAGCATCACCATATTCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTTCACCGCGACACGCTGGGCTTCTCGGA
GGCGCTGCGCTCGGCGTTGCGTGAAGACCCGGACGTGATTCTGGTCGGTGAAATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCTGCTGAAACCGGCCACCTGGTATTCGGCACGCTGCACACCACGTCAGCCGCCAAGACCATCGACCGGATC
GTCGACGTGTTTCCGGCTCAGGAAAAATCGATGATTCGCTCGATGCTCTCCGAATCGCTGCACGCCGTGGTCTCGCAGGC
GTTGCTCAAGAAGGTCGGCGGCGGGCGCGTGGCGGCGCACGAAATCATGATGGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAAGTGGCGCAGATGTATTCGTCGATCCAGACGGGTGGCTCGATGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTCAAGAAGGGGCTGATCACCCGCGAAAGCGCACGCGAACGCGCCAAGGTGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8INC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.535

100

0.895
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

78.779

100

0.788
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392

Multiple sequence alignment