Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CI104_RS04200 Genome accession   NZ_CP022695
Coordinates   819007..819987 (+) Length   326 a.a.
NCBI ID   WP_042324101.1    Uniprot ID   A0A8H9TWU6
Organism   Citrobacter farmeri strain AUSMDU00008141     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 814007..824987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CI104_RS04170 (CI104_04170) ansB 814597..815643 (+) 1047 WP_094464827.1 L-asparaginase 2 -
  CI104_RS04175 (CI104_04175) hemW 815683..816819 (-) 1137 WP_094464829.1 radical SAM family heme chaperone HemW -
  CI104_RS04180 (CI104_04180) - 816812..817405 (-) 594 WP_042324109.1 XTP/dITP diphosphatase -
  CI104_RS04185 (CI104_04185) yggU 817413..817703 (-) 291 WP_042324106.1 DUF167 family protein YggU -
  CI104_RS04190 (CI104_04190) - 817700..818266 (-) 567 WP_042324104.1 YggT family protein -
  CI104_RS04195 (CI104_04195) - 818285..818989 (-) 705 WP_094464831.1 YggS family pyridoxal phosphate-dependent enzyme -
  CI104_RS04200 (CI104_04200) pilT 819007..819987 (+) 981 WP_042324101.1 type IV pilus twitching motility protein PilT Machinery gene
  CI104_RS04205 (CI104_04205) ruvX 820001..820417 (-) 417 WP_042324100.1 Holliday junction resolvase RuvX -
  CI104_RS04210 (CI104_04210) - 820417..820980 (-) 564 WP_042324097.1 YqgE/AlgH family protein -
  CI104_RS04215 (CI104_04215) gshB 821171..822118 (-) 948 WP_042324095.1 glutathione synthase -
  CI104_RS04220 (CI104_04220) rsmE 822131..822862 (-) 732 WP_042324094.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CI104_RS04225 (CI104_04225) endA 822942..823649 (-) 708 WP_042324092.1 deoxyribonuclease I -
  CI104_RS04230 (CI104_04230) - 823741..824241 (-) 501 WP_042324090.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35446.59 Da        Isoelectric Point: 6.8261

>NTDB_id=241742 CI104_RS04200 WP_042324101.1 819007..819987(+) (pilT) [Citrobacter farmeri strain AUSMDU00008141]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMECAPFTAADITGLLADWLDEGQQAALQQNGQLDFAVSLVGNLRLRA
SAFHQRQGTSLALRLLPTKCPTLETLGTPSVLPELLSSENGLILVTGATGSGKSTTLAAMVTWLNQHVDGHILTLEDPIE
YVYASQRCLIQQREVGLHCASFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLEADKQEGRVALFELLINTPAAGNLIREGKSHQLPHIIQTGQQVGMMTFAQSLQQR
QAQGKL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=241742 CI104_RS04200 WP_042324101.1 819007..819987(+) (pilT) [Citrobacter farmeri strain AUSMDU00008141]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGGCGTATGGAATGCGCCCCTTTCACCGCAGCCGACATTACCGGACTGCTCGCGGATTGGCTGGATG
AAGGGCAGCAGGCGGCGTTACAGCAAAATGGACAACTGGACTTCGCCGTTTCGCTTGTGGGAAACCTGCGGTTGCGGGCC
AGCGCATTTCACCAGCGACAGGGAACGTCGCTGGCACTGCGACTGCTGCCGACGAAGTGCCCGACGCTGGAAACACTGGG
GACACCCTCCGTGCTGCCTGAACTGCTCAGCAGCGAGAACGGCTTAATTCTGGTGACGGGCGCCACGGGCAGTGGAAAAT
CGACTACCCTGGCGGCGATGGTGACGTGGCTGAATCAGCATGTTGATGGGCACATTCTGACGCTCGAAGATCCCATCGAA
TATGTCTACGCCAGCCAGCGTTGTCTGATTCAACAGCGTGAGGTGGGATTACACTGCGCTTCGTTTGCCGCAGGGTTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGGGAGCTTCGTGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGTGGCGCTGCGCAGGCGATCGAGCGGTTGGTTGATTCT
TTCCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCTGGCAGTCTGCGTGCCGTGCTGTCGCAAAAGCTGGAAGC
TGACAAGCAGGAGGGACGCGTTGCGCTGTTTGAACTTTTGATTAACACCCCGGCTGCGGGGAATTTAATTCGGGAAGGAA
AGTCCCACCAGTTGCCCCATATCATTCAGACCGGGCAGCAGGTGGGGATGATGACGTTTGCCCAGAGCCTGCAACAGCGA
CAGGCGCAGGGAAAGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

47.095

100

0.472
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Vibrio cholerae strain A1552

43.871

95.092

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383

Multiple sequence alignment