Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   CHH28_RS20205 Genome accession   NZ_CP022530
Coordinates   2073135..2073578 (-) Length   147 a.a.
NCBI ID   WP_094060137.1    Uniprot ID   A0A222FKQ5
Organism   Bacterioplanes sanyensis strain NV9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2068135..2078578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHH28_RS09750 (CHH28_09755) coaE 2068315..2068926 (-) 612 WP_094060134.1 dephospho-CoA kinase -
  CHH28_RS09755 (CHH28_09760) pilD 2069036..2069854 (-) 819 WP_332881238.1 A24 family peptidase Machinery gene
  CHH28_RS09760 (CHH28_09765) pilC 2069911..2071137 (-) 1227 WP_094060136.1 type II secretion system F family protein Machinery gene
  CHH28_RS09765 (CHH28_09770) pilB 2071202..2072890 (-) 1689 WP_094062036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CHH28_RS20205 (CHH28_09775) pilA/pilA1 2073135..2073578 (-) 444 WP_094060137.1 pilin Machinery gene
  CHH28_RS09775 (CHH28_09780) nadC 2073867..2074721 (-) 855 WP_094060138.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CHH28_RS09780 (CHH28_09785) - 2074889..2076265 (-) 1377 WP_094060139.1 alanine/glycine:cation symporter family protein -
  CHH28_RS09785 (CHH28_09790) ampD 2076593..2077159 (+) 567 WP_233243843.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CHH28_RS09790 (CHH28_09795) ampE 2077156..2078025 (+) 870 WP_094060141.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15052.42 Da        Isoelectric Point: 5.1033

>NTDB_id=240376 CHH28_RS20205 WP_094060137.1 2073135..2073578(-) (pilA/pilA1) [Bacterioplanes sanyensis strain NV9]
MKAQMQKGFTLIELMIVVAIIGILASIAVPAYQDYTARAQVAEGPKLVAGLQSDIAVLVAENGKIVEADYADGTPLKASA
GKLTGKYVKDVTVGASGVLTITFDAGAVAGKGMKLTPTISNGTITEWTCAPADTDGVEATHLPSGCR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=240376 CHH28_RS20205 WP_094060137.1 2073135..2073578(-) (pilA/pilA1) [Bacterioplanes sanyensis strain NV9]
ATGAAAGCACAAATGCAAAAGGGTTTTACCCTGATTGAATTGATGATCGTAGTAGCGATCATCGGTATTCTGGCCTCTAT
TGCTGTACCGGCCTACCAGGACTATACCGCCCGCGCTCAAGTTGCCGAAGGCCCTAAATTAGTCGCAGGACTGCAATCTG
ACATTGCTGTCCTAGTTGCTGAGAATGGCAAAATTGTTGAAGCAGACTATGCAGATGGCACGCCGCTAAAAGCTTCAGCC
GGCAAACTAACTGGCAAATATGTAAAAGACGTGACAGTAGGAGCCAGTGGTGTGCTCACCATTACCTTTGACGCAGGCGC
TGTGGCCGGTAAAGGTATGAAGCTGACACCAACCATCTCCAACGGTACTATCACCGAGTGGACTTGTGCACCAGCAGACA
CCGATGGTGTCGAAGCAACACACTTGCCAAGCGGCTGTCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-28)

Pilin (bacterial filament) (Pilin)

(37-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222FKQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

52.288

100

0.544
  pilE Neisseria gonorrhoeae MS11

42.5

100

0.463
  pilA2 Legionella pneumophila str. Paris

45.139

97.959

0.442
  pilA2 Legionella pneumophila strain ERS1305867

45.139

97.959

0.442
  pilE Neisseria gonorrhoeae strain FA1090

40

100

0.435
  pilA Ralstonia pseudosolanacearum GMI1000

36.994

100

0.435
  comP Acinetobacter baylyi ADP1

40.909

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.645

100

0.429
  pilA Pseudomonas aeruginosa PAK

37.58

100

0.401
  pilA Vibrio cholerae C6706

37.333

100

0.381
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.333

100

0.381
  pilA Vibrio cholerae strain A1552

37.333

100

0.381
  pilA Acinetobacter baumannii strain A118

36.806

97.959

0.361

Multiple sequence alignment