Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CHH28_RS09765 Genome accession   NZ_CP022530
Coordinates   2071202..2072890 (-) Length   562 a.a.
NCBI ID   WP_094062036.1    Uniprot ID   A0A222FQU6
Organism   Bacterioplanes sanyensis strain NV9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2066202..2077890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHH28_RS09740 (CHH28_09745) - 2067724..2068134 (+) 411 WP_094060132.1 copper chaperone PCu(A)C -
  CHH28_RS09745 (CHH28_09750) yacG 2068131..2068325 (-) 195 WP_094060133.1 DNA gyrase inhibitor YacG -
  CHH28_RS09750 (CHH28_09755) coaE 2068315..2068926 (-) 612 WP_094060134.1 dephospho-CoA kinase -
  CHH28_RS09755 (CHH28_09760) pilD 2069036..2069854 (-) 819 WP_332881238.1 A24 family peptidase Machinery gene
  CHH28_RS09760 (CHH28_09765) pilC 2069911..2071137 (-) 1227 WP_094060136.1 type II secretion system F family protein Machinery gene
  CHH28_RS09765 (CHH28_09770) pilB 2071202..2072890 (-) 1689 WP_094062036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CHH28_RS20205 (CHH28_09775) pilA/pilA1 2073135..2073578 (-) 444 WP_094060137.1 pilin Machinery gene
  CHH28_RS09775 (CHH28_09780) nadC 2073867..2074721 (-) 855 WP_094060138.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CHH28_RS09780 (CHH28_09785) - 2074889..2076265 (-) 1377 WP_094060139.1 alanine/glycine:cation symporter family protein -
  CHH28_RS09785 (CHH28_09790) ampD 2076593..2077159 (+) 567 WP_233243843.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61448.28 Da        Isoelectric Point: 4.7388

>NTDB_id=240366 CHH28_RS09765 WP_094062036.1 2071202..2072890(-) (pilB) [Bacterioplanes sanyensis strain NV9]
MPALTGLPKRLVENQLLEAEQAAALLDKAKKESISFTALALSEGKVKAVTMAQLASEEFGLPLFDLAAIEQDSMPVDQVS
RELIEKHRVIPLFQRGNRLFVGLSDPSNILAVDEIKFNTGMSVDMVLVEEDKLAIAVDRFISDSDTGFGDLGDDEDLDLE
SPQEEDKKQDENDGADDAPIVKFINKMLLDAVKQGSSDLHFEPYEKRYRVRYRTDGVLHEVASPPVNLAGKIAARLKVMS
QMDISERRIPQDGRIKMKISKNRAIDFRVNTLPTLFGEKIVLRILDPSSAQLGIDMLGYEPEQKKLYMDALHKPQGMILV
TGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVSAKVGLDFAAALRSFLRQDPDVVMVGEIRDLETAS
IAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPAFNIATSVTLIIAQRLARRLCKSCKKPANIPKEALYEAGFTDALLA
EAEIYQPVGCSECSDGYKGRVGIYEVVRITDSISRIIMENGNSIQISDAARKEGFNDLRTSALMKVAQGVTSLEEANRVT
TD

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=240366 CHH28_RS09765 WP_094062036.1 2071202..2072890(-) (pilB) [Bacterioplanes sanyensis strain NV9]
ATGCCTGCGTTAACCGGCCTCCCCAAGCGACTGGTTGAAAACCAACTTTTAGAAGCAGAGCAAGCCGCCGCTCTACTGGA
CAAAGCAAAAAAAGAGAGTATCAGCTTTACCGCCCTAGCCTTAAGCGAAGGTAAAGTCAAAGCAGTGACTATGGCCCAAC
TGGCCTCAGAAGAGTTTGGCTTGCCCTTATTTGACCTTGCCGCCATCGAGCAAGATTCCATGCCAGTAGACCAGGTCAGC
CGTGAACTGATTGAAAAGCACCGTGTGATTCCCCTGTTTCAGCGAGGCAATCGCTTATTCGTGGGGCTCAGCGACCCTTC
GAACATATTGGCTGTTGATGAAATCAAGTTTAATACCGGCATGTCCGTTGACATGGTACTGGTGGAGGAAGACAAGCTCG
CCATCGCGGTAGATCGCTTTATCTCTGACAGCGATACTGGCTTTGGTGATCTGGGCGATGACGAAGACCTAGATCTTGAA
AGCCCTCAAGAAGAAGACAAAAAGCAGGATGAGAACGACGGCGCCGATGATGCCCCCATCGTTAAGTTTATTAACAAAAT
GCTGCTGGATGCGGTCAAGCAAGGCTCCTCTGACTTGCACTTTGAGCCCTACGAAAAGCGCTACCGGGTACGCTATCGTA
CCGATGGCGTTCTGCATGAAGTGGCCAGCCCGCCGGTAAACCTAGCCGGTAAAATCGCCGCACGCCTAAAGGTGATGTCG
CAAATGGATATTTCTGAGCGGCGTATTCCACAAGATGGACGTATCAAAATGAAAATTTCTAAGAATCGTGCCATCGACTT
TCGGGTAAACACCTTGCCTACCTTGTTTGGCGAGAAGATCGTCCTGCGGATTCTTGACCCATCTTCCGCCCAGTTAGGCA
TTGATATGCTCGGCTATGAGCCAGAGCAGAAAAAACTTTATATGGATGCCTTGCATAAGCCCCAGGGCATGATTTTGGTG
ACAGGGCCTACCGGCTCGGGTAAAACCGTCTCGCTCTATACTGGCCTGAACATCCTCAATGTCGAAGGTACCAATATCTC
CACCGCTGAAGACCCAGTAGAGATTAATCTTGAGGGCATCAACCAGGTCAATGTCAGTGCCAAAGTCGGCCTCGATTTTG
CTGCCGCCTTGCGCTCCTTCTTGCGCCAAGACCCTGATGTAGTCATGGTGGGTGAGATTCGTGACTTGGAAACCGCCTCT
ATCGCCATCAAAGCCGCTCAAACCGGTCACTTGGTGCTTTCCACACTACACACCAACAGTGCCGCCGAAACCCTGACCCG
CTTGGCCAATATGGGCGTACCTGCCTTTAATATCGCCACTTCCGTCACCCTTATTATTGCCCAGCGCTTAGCCCGCCGGT
TATGCAAGAGTTGCAAAAAGCCAGCGAACATCCCCAAAGAAGCCTTGTATGAAGCCGGCTTTACCGATGCACTACTAGCA
GAAGCAGAAATATACCAGCCTGTGGGCTGTAGCGAGTGCAGCGATGGTTATAAAGGCCGGGTGGGTATTTATGAAGTAGT
CAGAATCACAGATTCAATCTCACGCATCATTATGGAAAACGGCAACTCCATTCAGATTTCTGACGCAGCGCGCAAAGAGG
GCTTTAATGACCTACGCACCTCCGCCTTAATGAAGGTGGCACAAGGGGTGACTAGCCTGGAAGAGGCGAACCGTGTCACA
ACCGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-144)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222FQU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.234

100

0.625
  pilB Acinetobacter baylyi ADP1

60.494

100

0.61
  pilB Legionella pneumophila strain ERS1305867

53.451

100

0.537
  pilB Vibrio cholerae strain A1552

50.088

100

0.509
  pilF Neisseria gonorrhoeae MS11

50.266

100

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.631

98.754

0.5
  pilB Vibrio campbellii strain DS40M4

49.822

100

0.498
  pilB/pilB1 Synechocystis sp. PCC 6803

38.01

100

0.415

Multiple sequence alignment