Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MYMAC_RS28110 Genome accession   NZ_CP022203
Coordinates   6987971..6989086 (-) Length   371 a.a.
NCBI ID   WP_095960313.1    Uniprot ID   A0A250K1I0
Organism   Corallococcus macrosporus DSM 14697     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6982971..6994086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYMAC_RS28095 (MYMAC_005576) - 6983633..6985021 (-) 1389 WP_420810019.1 sigma-54-dependent transcriptional regulator -
  MYMAC_RS28100 (MYMAC_005577) - 6985066..6986598 (-) 1533 WP_095961713.1 two-component system sensor histidine kinase NtrB -
  MYMAC_RS28105 (MYMAC_005578) - 6986660..6987913 (-) 1254 WP_095960312.1 type II secretion system F family protein -
  MYMAC_RS28110 (MYMAC_005579) pilT 6987971..6989086 (-) 1116 WP_095960313.1 type IV pilus twitching motility protein PilT Machinery gene
  MYMAC_RS28115 (MYMAC_005580) pilB 6989129..6990829 (-) 1701 WP_013942229.1 type IV-A pilus assembly ATPase PilB -
  MYMAC_RS28120 (MYMAC_005581) - 6991034..6991960 (-) 927 WP_095960314.1 bifunctional riboflavin kinase/FAD synthetase -
  MYMAC_RS28125 (MYMAC_005582) trmB 6992036..6992656 (+) 621 WP_013942231.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  MYMAC_RS28130 (MYMAC_005583) - 6992764..6993807 (+) 1044 WP_095960315.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40623.97 Da        Isoelectric Point: 8.2933

>NTDB_id=237521 MYMAC_RS28110 WP_095960313.1 6987971..6989086(-) (pilT) [Corallococcus macrosporus DSM 14697]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKSRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDIVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQALVAKAGGPGRILALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRLITQDEAFGRSTDPEELRNILATGGGTLPGMQRPGGGAGGR

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=237521 MYMAC_RS28110 WP_095960313.1 6987971..6989086(-) (pilT) [Corallococcus macrosporus DSM 14697]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTCCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGACAACGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCGAACATCTTCATGCAGCGTGGCGCGGTCGCCGCGGCGTTCCGGACCATTCCCTTCAAGATCCTGACGTT
CCAGGAGCTGGGCCTGCCGCCCGTCGTCGCGGAGCTGGTGAAGAAGTCGCGCGGGCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAATCAGCGCGAGGTGGGCGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACATCGTGCTCGTCGGCGAGCTCCGCGACCTGGAGACGATTGAAG
CGGCGCTCACCATCGCGGAGACAGGCCACATCTGCTACGCGACGCTACATACCAATAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTCCGCGCGCAGCTCTCCTTCGTGTTGGAGGGCGTGATGAGCCA
GGCGCTGGTGGCGAAGGCGGGCGGCCCCGGCCGCATCCTGGCGCTGGAGGTGATGGTGCCCAACCCCGCAATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCGAAGTTCGGCATGCAGACCTTCAAC
CAGGCCCTGGCGGCGCTGCTGCTGCGGCGGCTCATCACCCAGGACGAGGCCTTCGGCCGCTCCACCGACCCGGAGGAGCT
GCGCAACATCCTGGCCACCGGCGGCGGGACCCTGCCCGGGATGCAGCGGCCAGGCGGGGGAGCGGGTGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A250K1I0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.731

90.296

0.485
  pilT Legionella pneumophila strain Lp02

53.731

90.296

0.485
  pilT Pseudomonas stutzeri DSM 10701

51.594

92.992

0.48
  pilT Pseudomonas aeruginosa PAK

51.594

92.992

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

92.992

0.477
  pilT Acinetobacter baylyi ADP1

51.014

92.992

0.474
  pilT Acinetobacter baumannii D1279779

51.014

92.992

0.474
  pilT Acinetobacter baumannii strain A118

51.014

92.992

0.474
  pilT Acinetobacter nosocomialis M2

51.014

92.992

0.474
  pilT Vibrio cholerae strain A1552

48.503

90.027

0.437
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

90.027

0.437
  pilT Neisseria meningitidis 8013

44.575

91.914

0.41
  pilT Neisseria gonorrhoeae MS11

44.575

91.914

0.41
  pilU Vibrio cholerae strain A1552

43.059

95.148

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.96

95.418

0.391
  pilU Acinetobacter baylyi ADP1

39.769

93.531

0.372

Multiple sequence alignment