Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CEQ75_RS13350 Genome accession   NZ_CP022121
Coordinates   2752891..2753937 (-) Length   348 a.a.
NCBI ID   WP_089611398.1    Uniprot ID   -
Organism   Dehalobacterium formicoaceticum strain DMC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2747891..2758937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEQ75_RS13320 - 2748412..2748924 (-) 513 WP_089611388.1 PilW family protein -
  CEQ75_RS13325 - 2748933..2749328 (-) 396 WP_157677471.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CEQ75_RS13330 - 2749454..2749915 (-) 462 WP_198306543.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CEQ75_RS13335 - 2750122..2750867 (-) 746 Protein_2627 prepilin peptidase -
  CEQ75_RS19240 - 2750957..2751343 (-) 387 WP_089611394.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CEQ75_RS13345 - 2751544..2752758 (-) 1215 WP_198306544.1 type II secretion system F family protein -
  CEQ75_RS13350 pilT 2752891..2753937 (-) 1047 WP_089611398.1 type IV pilus twitching motility protein PilT Machinery gene
  CEQ75_RS13355 - 2754078..2755775 (-) 1698 WP_089611400.1 ATPase, T2SS/T4P/T4SS family -
  CEQ75_RS13360 - 2755914..2756363 (-) 450 WP_157677472.1 MerR family transcriptional regulator -
  CEQ75_RS13365 - 2756558..2757427 (-) 870 WP_198306545.1 shikimate dehydrogenase -
  CEQ75_RS13370 - 2757502..2757744 (-) 243 WP_089611407.1 hypothetical protein -
  CEQ75_RS13375 - 2757919..2758488 (-) 570 WP_338032026.1 YqeG family HAD IIIA-type phosphatase -
  CEQ75_RS13380 - 2758442..2758699 (-) 258 WP_089611409.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38364.19 Da        Isoelectric Point: 6.8841

>NTDB_id=237024 CEQ75_RS13350 WP_089611398.1 2752891..2753937(-) (pilT) [Dehalobacterium formicoaceticum strain DMC]
MNISEILTKAVEMKASDVHLTAGRPPLFRLLGDLHAQEQWKMLLPPDTEALCREMFTPRLSDEMDGQGQVDFAYGLAGLG
RFRVNVFKQRNSFCAAIRVINPEIVSLEELNLPPVVGELARRPRGMVLVTGPTGSGKSTTLASMIDLVNIEYKKHILTLE
DPIEFLHKHKNCIVNQREIGTDTHSFSDGLRAGLREDPDVILVGEMRDPETISIAVTAAETGHLVLTTLHTSSAAQTVER
IIDVFPPHQQQQIRVQLASTIQGVISQTLLKRADGKGRIAAAEILIGTPAVRNLIREGKTHQLNSMIQTGGQYGMQSLDS
TLRKMIHEGTILMKEALTQNANFQAQSI

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=237024 CEQ75_RS13350 WP_089611398.1 2752891..2753937(-) (pilT) [Dehalobacterium formicoaceticum strain DMC]
ATGAATATCAGTGAAATTTTGACGAAGGCTGTAGAAATGAAAGCCTCAGACGTACACCTGACCGCCGGGCGGCCGCCTCT
TTTTCGTTTGCTGGGAGATTTGCATGCCCAGGAGCAATGGAAAATGCTCCTGCCCCCGGATACGGAAGCTTTGTGTCGGG
AAATGTTTACCCCACGATTGTCTGATGAGATGGATGGTCAGGGACAGGTGGACTTTGCCTATGGCCTCGCCGGATTAGGC
CGTTTCCGGGTCAATGTCTTTAAGCAGCGCAATAGTTTCTGTGCGGCTATCCGGGTGATCAATCCGGAGATCGTCAGTCT
GGAGGAACTAAATCTTCCTCCTGTGGTGGGAGAGCTTGCCCGGAGACCACGGGGCATGGTACTGGTGACGGGACCAACGG
GAAGCGGTAAATCCACCACCCTGGCCTCGATGATCGATTTGGTCAATATAGAATACAAAAAACATATTCTCACCTTGGAG
GATCCTATAGAATTTCTTCATAAACATAAGAATTGTATCGTCAACCAAAGGGAAATCGGTACTGATACCCATTCTTTTTC
AGATGGATTGCGCGCCGGTCTCCGGGAGGACCCGGATGTAATTTTAGTGGGAGAAATGCGTGATCCGGAAACCATTTCCA
TTGCCGTAACCGCTGCAGAAACCGGCCATTTGGTGTTAACCACCCTCCATACTTCCAGTGCCGCTCAGACGGTGGAGAGA
ATCATTGACGTTTTTCCTCCACATCAGCAACAACAGATTCGAGTACAATTAGCCTCCACCATCCAGGGGGTTATTTCTCA
AACTCTCTTAAAACGGGCAGACGGAAAAGGACGCATTGCAGCGGCAGAGATATTGATCGGTACGCCGGCTGTGCGTAATT
TAATCAGGGAAGGGAAAACACACCAATTAAATTCCATGATTCAGACCGGGGGGCAATATGGCATGCAAAGCCTGGACTCT
ACCTTAAGAAAGATGATTCATGAGGGTACGATTTTGATGAAAGAAGCTTTGACACAAAATGCAAATTTCCAAGCGCAATC
TATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(91-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.522

96.839

0.509
  pilT Pseudomonas stutzeri DSM 10701

49.143

100

0.494
  pilT Neisseria meningitidis 8013

48.58

100

0.491
  pilT Legionella pneumophila strain ERS1305867

50.746

96.264

0.489
  pilT Legionella pneumophila strain Lp02

50.746

96.264

0.489
  pilT Neisseria gonorrhoeae MS11

48.295

100

0.489
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.853

97.414

0.486
  pilT Vibrio cholerae strain A1552

49.853

97.414

0.486
  pilT Acinetobacter baylyi ADP1

50.149

96.264

0.483
  pilT Pseudomonas aeruginosa PAK

50.149

96.264

0.483
  pilT Acinetobacter nosocomialis M2

48.521

97.126

0.471
  pilT Acinetobacter baumannii D1279779

48.521

97.126

0.471
  pilT Acinetobacter baumannii strain A118

48.521

97.126

0.471
  pilU Vibrio cholerae strain A1552

42.433

96.839

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.246

96.839

0.399
  pilU Acinetobacter baylyi ADP1

40.708

97.414

0.397

Multiple sequence alignment