Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CYFUS_RS51825 Genome accession   NZ_CP022098
Coordinates   6599822..6600877 (-) Length   351 a.a.
NCBI ID   WP_198316939.1    Uniprot ID   -
Organism   Cystobacter fuscus strain DSM 52655     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6594822..6605877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYFUS_RS26895 (CYFUS_005308) - 6596088..6596855 (-) 768 WP_095987834.1 hypothetical protein -
  CYFUS_RS54375 (CYFUS_005309) - 6596941..6597660 (-) 720 WP_095987835.1 Imm52 family immunity protein -
  CYFUS_RS26905 (CYFUS_005310) - 6597657..6599009 (-) 1353 WP_157758674.1 Tox-REase-5 domain-containing protein -
  CYFUS_RS51825 pilT 6599822..6600877 (-) 1056 WP_198316939.1 type IV pilus twitching motility protein PilT Machinery gene
  CYFUS_RS51830 - 6600789..6601490 (-) 702 WP_198316086.1 hypothetical protein -
  CYFUS_RS26915 (CYFUS_005311) - 6601497..6601943 (-) 447 WP_095992247.1 hypothetical protein -
  CYFUS_RS26920 (CYFUS_005312) - 6602205..6603017 (+) 813 WP_095987837.1 siderophore-interacting protein -
  CYFUS_RS26925 (CYFUS_005313) - 6603075..6603968 (+) 894 WP_095987838.1 hypothetical protein -
  CYFUS_RS26930 (CYFUS_005314) - 6603965..6604687 (+) 723 WP_095987839.1 hypothetical protein -
  CYFUS_RS26935 (CYFUS_005315) - 6604801..6605502 (+) 702 WP_095987840.1 head GIN domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39491.58 Da        Isoelectric Point: 7.0163

>NTDB_id=236704 CYFUS_RS51825 WP_198316939.1 6599822..6600877(-) (pilT) [Cystobacter fuscus strain DSM 52655]
MLDKKASDLHMSSQVVPMLRIDGDMVPQDDYRELSHDRLKAMLWSIAPEKNKKQWEETRDTDFAYETDRARFRVNVFEDR
KGIGSVMRQIPTKIMTAEDMGLSKHILDLCFLSKGLVLVTGPTGSGKSTTLAAMIDYINRNREDHIITIEDPIEFVHPNK
KCLVNQREVHVHTHGFKNALRAALREDPDIVLVGEMRDLETIAIAIETAETGHLVFGTLHTNTAPSTVDRIIDQFPADRQ
EQIRMMLSESLKGVISQMLVKKIGGGRVPAQEVLLCTSSVANLIREGKTFQIPSIMQTSRGIGMSTLNDSLFDLVKRKLC
EPNDAYIKAVAKGEFKQMLERAGYKLDLPTS

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=236704 CYFUS_RS51825 WP_198316939.1 6599822..6600877(-) (pilT) [Cystobacter fuscus strain DSM 52655]
ATGCTCGACAAGAAGGCCTCGGACCTTCACATGTCCAGCCAGGTGGTGCCCATGCTGCGCATCGACGGCGACATGGTGCC
CCAGGATGACTACCGCGAGCTGTCGCATGACCGGCTCAAGGCCATGCTCTGGAGCATCGCGCCGGAGAAGAACAAGAAGC
AGTGGGAGGAGACGCGCGACACGGACTTCGCCTACGAGACCGATCGCGCCCGCTTCCGCGTCAACGTCTTCGAGGATCGC
AAGGGCATCGGCTCGGTGATGCGGCAGATCCCCACGAAGATCATGACGGCCGAGGACATGGGTCTGTCCAAGCACATCCT
CGATCTGTGCTTCCTGAGCAAGGGCCTGGTGCTCGTCACCGGCCCCACCGGCTCGGGCAAGTCCACCACGCTCGCGGCGA
TGATCGACTACATCAATCGCAACCGCGAGGATCACATCATCACGATCGAGGACCCGATCGAGTTCGTCCACCCGAACAAG
AAATGTCTGGTCAACCAGCGTGAGGTCCACGTCCACACGCACGGCTTCAAGAACGCGCTCCGCGCCGCGCTGCGCGAGGA
CCCAGACATCGTGCTCGTGGGCGAAATGCGAGACCTGGAGACGATCGCCATCGCCATCGAAACGGCGGAAACGGGCCACC
TCGTCTTCGGCACCCTGCACACCAACACGGCGCCCTCGACGGTGGACCGCATCATCGACCAGTTCCCCGCGGACCGTCAG
GAGCAGATCCGCATGATGCTCTCCGAGTCCCTCAAGGGCGTCATCTCGCAGATGCTCGTGAAGAAGATTGGTGGAGGCCG
CGTGCCCGCGCAGGAGGTGCTCCTGTGCACCAGCTCCGTCGCCAACCTCATCCGCGAGGGCAAGACGTTCCAGATTCCGT
CCATCATGCAGACGTCGCGCGGCATCGGCATGTCCACGCTCAACGACTCGCTGTTCGACCTCGTCAAGCGCAAGCTGTGC
GAGCCGAACGACGCCTACATCAAGGCCGTGGCCAAGGGCGAGTTCAAGCAGATGCTCGAGCGCGCGGGCTACAAGCTCGA
TCTGCCCACGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(2-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

56

92.593

0.519
  pilT Acinetobacter baumannii strain A118

56

92.593

0.519
  pilT Acinetobacter nosocomialis M2

56

92.593

0.519
  pilT Acinetobacter baylyi ADP1

54.819

94.587

0.519
  pilT Pseudomonas aeruginosa PAK

55.077

92.593

0.51
  pilT Pseudomonas stutzeri DSM 10701

54.769

92.593

0.507
  pilT Legionella pneumophila strain Lp02

52.711

94.587

0.499
  pilT Legionella pneumophila strain ERS1305867

52.711

94.587

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.844

98.575

0.481
  pilT Neisseria meningitidis 8013

51.524

93.447

0.481
  pilT Neisseria gonorrhoeae MS11

51.22

93.447

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.769

92.593

0.47
  pilT Vibrio cholerae strain A1552

50.769

92.593

0.47
  pilU Acinetobacter baylyi ADP1

39.943

100

0.402
  pilU Vibrio cholerae strain A1552

40.762

97.151

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.474

97.436

0.385

Multiple sequence alignment