Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CYFUS_RS40705 Genome accession   NZ_CP022098
Coordinates   10056338..10057447 (-) Length   369 a.a.
NCBI ID   WP_095990103.1    Uniprot ID   A0A250JF89
Organism   Cystobacter fuscus strain DSM 52655     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 10051338..10062447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYFUS_RS40690 (CYFUS_008041) - 10052005..10053393 (-) 1389 WP_095990101.1 sigma-54-dependent transcriptional regulator -
  CYFUS_RS40695 (CYFUS_008042) - 10053416..10055017 (-) 1602 WP_232537092.1 two-component system sensor histidine kinase NtrB -
  CYFUS_RS40700 (CYFUS_008043) - 10055034..10056287 (-) 1254 WP_095990102.1 type II secretion system F family protein -
  CYFUS_RS40705 (CYFUS_008044) pilT 10056338..10057447 (-) 1110 WP_095990103.1 type IV pilus twitching motility protein PilT Machinery gene
  CYFUS_RS40710 (CYFUS_008045) pilB 10057491..10059194 (-) 1704 WP_095990104.1 type IV-A pilus assembly ATPase PilB -
  CYFUS_RS40715 (CYFUS_008046) - 10059378..10060304 (-) 927 WP_095990105.1 bifunctional riboflavin kinase/FAD synthetase -
  CYFUS_RS40720 (CYFUS_008047) trmB 10060395..10061015 (+) 621 WP_095990106.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  CYFUS_RS40725 (CYFUS_008048) - 10061175..10062248 (+) 1074 WP_095990107.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40457.62 Da        Isoelectric Point: 8.2829

>NTDB_id=236702 CYFUS_RS40705 WP_095990103.1 10056338..10057447(-) (pilT) [Cystobacter fuscus strain DSM 52655]
MNLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAQLSPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLSR
FRANVYMQRGAVAGAFRTIPFKILTFQELGLPPVVGELVKRPRGLILVTGPTGSGKSTTLASMVDKINTDRHEHIMTIED
PIEYLHPHKNCLVNQREVGADTRSFKTALKYILRQDPDVVLVGELRDLETIEAALVIAETGHTCYATLHTNSAVQTINRV
LDVFPPYQQPQVRAQLSFVLEGVMSQSLIAKAGSPGRVLALEVMIPNPAIRNLIREDKIHQVYSSMQVGQAKYGMQTFNQ
ALAALLARRLITQDEAFGRSSDPDELRNILAGGTAAGMPGQRPAGPAGR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=236702 CYFUS_RS40705 WP_095990103.1 10056338..10057447(-) (pilT) [Cystobacter fuscus strain DSM 52655]
GTGAATCTGCATCAGCTGCTCAAGGCGATGGTCGAGAAGGGTGCTTCCGACCTCCACATCACCACTGGCTCCCCGCCCCA
GCTGCGCGTGGACGGCGAGCTGGTGCCGCTCAAGACGGCCCAGCTATCGCCGGTGGAGACCAAGCAGCTCTGCTACTCCA
TCCTCACCGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTCTCCTTCGGCGTGAAGGGGCTGTCGCGC
TTCCGCGCCAACGTCTACATGCAGCGTGGCGCGGTGGCGGGGGCGTTCCGCACCATTCCCTTCAAGATCCTCACCTTCCA
GGAGCTGGGTCTGCCCCCGGTGGTGGGGGAGCTCGTCAAGCGCCCGCGCGGCCTCATCCTGGTGACGGGTCCCACGGGCT
CGGGCAAGTCCACGACGCTCGCGTCGATGGTCGACAAGATCAACACCGATCGTCATGAGCACATCATGACGATCGAGGAT
CCGATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTCAACCAGCGCGAGGTGGGCGCCGACACGCGCAGCTTCAAGAC
GGCGCTCAAGTACATCCTGCGCCAGGATCCGGACGTGGTGCTGGTGGGCGAGCTGCGCGACCTGGAGACGATCGAGGCGG
CGCTCGTCATCGCCGAGACGGGCCACACCTGCTACGCCACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGGGTG
CTGGACGTGTTCCCGCCGTACCAGCAGCCCCAGGTGCGCGCCCAGCTGTCGTTCGTGCTGGAGGGAGTGATGAGTCAGTC
GCTCATCGCCAAGGCGGGCTCTCCGGGCCGCGTGCTGGCGCTGGAGGTCATGATTCCCAACCCCGCCATCCGCAACCTCA
TCCGCGAGGACAAGATCCATCAGGTCTACTCGTCGATGCAGGTGGGCCAGGCCAAGTACGGCATGCAGACCTTCAACCAG
GCCCTGGCGGCGCTGCTGGCGCGGCGCCTCATCACCCAGGACGAGGCATTCGGCCGTTCCTCGGATCCGGACGAGCTGCG
CAACATCCTCGCGGGCGGCACCGCCGCTGGCATGCCTGGTCAACGGCCCGCCGGTCCCGCGGGGCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A250JF89

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.272

99.729

0.501
  pilT Legionella pneumophila strain ERS1305867

53.392

91.87

0.491
  pilT Legionella pneumophila strain Lp02

53.392

91.87

0.491
  pilT Pseudomonas aeruginosa PAK

51.576

94.58

0.488
  pilT Pseudomonas stutzeri DSM 10701

50.867

93.767

0.477
  pilT Acinetobacter baylyi ADP1

50.578

93.767

0.474
  pilT Acinetobacter baumannii strain A118

50.289

93.767

0.472
  pilT Acinetobacter baumannii D1279779

50.289

93.767

0.472
  pilT Acinetobacter nosocomialis M2

50

93.767

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.358

90.786

0.439
  pilT Vibrio cholerae strain A1552

48.358

90.786

0.439
  pilT Neisseria gonorrhoeae MS11

44.22

93.767

0.415
  pilT Neisseria meningitidis 8013

44.22

93.767

0.415
  pilU Vibrio cholerae strain A1552

42.857

94.851

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.678

95.935

0.39
  pilU Acinetobacter baylyi ADP1

40.517

94.309

0.382

Multiple sequence alignment