Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CEP86_RS10125 Genome accession   NZ_CP022097
Coordinates   2204191..2205225 (+) Length   344 a.a.
NCBI ID   WP_011064011.1    Uniprot ID   Q4K4D8
Organism   Pseudomonas protegens strain FDAARGOS_307     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2199191..2210225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP86_RS10095 (CEP86_10055) metW 2199609..2200229 (-) 621 WP_011064017.1 methionine biosynthesis protein MetW -
  CEP86_RS10100 (CEP86_10060) - 2200237..2201376 (-) 1140 WP_011064016.1 homoserine O-acetyltransferase -
  CEP86_RS10105 (CEP86_10065) - 2201540..2201833 (-) 294 WP_011064015.1 DUF167 domain-containing protein -
  CEP86_RS10110 (CEP86_10070) - 2201848..2202438 (-) 591 WP_011064014.1 YggT family protein -
  CEP86_RS10115 (CEP86_10075) proC 2202449..2203267 (-) 819 WP_011064013.1 pyrroline-5-carboxylate reductase -
  CEP86_RS10120 (CEP86_10080) - 2203302..2204000 (-) 699 WP_011064012.1 YggS family pyridoxal phosphate-dependent enzyme -
  CEP86_RS10125 (CEP86_10085) pilT 2204191..2205225 (+) 1035 WP_011064011.1 type IV pilus twitching motility protein PilT Machinery gene
  CEP86_RS10130 (CEP86_10090) - 2205304..2205927 (-) 624 WP_011064010.1 C40 family peptidase -
  CEP86_RS10135 (CEP86_10095) - 2206178..2206597 (+) 420 WP_011064009.1 TM2 domain-containing protein -
  CEP86_RS10140 (CEP86_10100) - 2206725..2207996 (-) 1272 WP_011064008.1 dihydroorotase -
  CEP86_RS10145 (CEP86_10105) - 2207993..2208997 (-) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  CEP86_RS10150 (CEP86_10110) pyrR 2209023..2209529 (-) 507 WP_011064006.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  CEP86_RS10155 (CEP86_10115) ruvX 2209645..2210082 (-) 438 WP_011064005.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37389.14 Da        Isoelectric Point: 7.0293

>NTDB_id=236674 CEP86_RS10125 WP_011064011.1 2204191..2205225(+) (pilT) [Pseudomonas protegens strain FDAARGOS_307]
MDITELLRLSASRGASDLHLSAGLAPMLRIDGDIRPLPGPALEGPQVQALIHELMSEAQRQAFAACNDLDFAYELPGVAR
FRVNAFRHARGVGAVLRLIPARVQSLEELGLGEVFRQITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNRNRQQHILTIED
PIEFVHPQKNCLVHQREVQRDTQSFATALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLVKKVGGGRVAAHEIMLGSPAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISRDQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=236674 CEP86_RS10125 WP_011064011.1 2204191..2205225(+) (pilT) [Pseudomonas protegens strain FDAARGOS_307]
ATGGACATCACCGAACTCTTGCGCTTGAGCGCTTCCCGGGGCGCTTCGGACCTGCATCTGTCCGCCGGGCTGGCGCCGAT
GTTGCGCATCGATGGCGATATTCGCCCGCTGCCGGGCCCGGCCCTGGAAGGGCCACAGGTGCAGGCGCTGATCCATGAAC
TGATGAGCGAAGCACAGCGCCAGGCGTTCGCCGCCTGCAATGACCTGGATTTCGCCTATGAACTGCCGGGTGTGGCGCGG
TTTCGGGTCAATGCCTTCCGGCACGCCCGTGGCGTCGGTGCGGTGCTGCGCCTGATTCCCGCGCGGGTACAGAGCCTGGA
GGAGCTGGGGCTGGGGGAAGTGTTTCGGCAGATTACCGAGGCGCCCCGCGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACACTGGCGGCGATGATCGACCACCTGAACCGGAACCGCCAGCAGCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCATCCGCAGAAGAACTGCCTGGTGCACCAGCGCGAAGTGCAGCGCGATACCCAGAGCTTCGCCAC
GGCCTTGCGTGCGGCGCTGCGGGAAGATCCCGACGTGATCCTGCTGGGCGAACTGCGGGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGGCACCTGGTACTGGCCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGCTG
GTGGACGTGTTTCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCAGTGGTCGCGCAAGT
GCTGGTGAAGAAGGTGGGCGGTGGCCGGGTAGCGGCGCACGAGATCATGCTGGGCAGCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACGGGCGGCGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCATTGCTCGGGCAGGGGTTGATCAGCCGCGATCAGGCGCGGGAGCAGGCGCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4K4D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.93

100

0.709
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter baumannii D1279779

66.279

100

0.663
  pilT Acinetobacter baumannii strain A118

66.279

100

0.663
  pilT Acinetobacter nosocomialis M2

66.279

100

0.663
  pilT Acinetobacter baylyi ADP1

65.698

100

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.353

98.837

0.616
  pilT Vibrio cholerae strain A1552

62.353

98.837

0.616
  pilT Legionella pneumophila strain Lp02

61.834

98.256

0.608
  pilT Legionella pneumophila strain ERS1305867

61.834

98.256

0.608
  pilT Neisseria meningitidis 8013

58.261

100

0.584
  pilT Neisseria gonorrhoeae MS11

57.971

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

38.261

100

0.384

Multiple sequence alignment