Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CEP66_RS11815 Genome accession   NZ_CP022073
Coordinates   2423199..2424179 (+) Length   326 a.a.
NCBI ID   WP_058668078.1    Uniprot ID   -
Organism   Citrobacter koseri strain FDAARGOS_287     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418199..2429179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP66_RS11785 (CEP66_11725) ansB 2418710..2419756 (+) 1047 WP_049010789.1 L-asparaginase 2 -
  CEP66_RS11790 (CEP66_11730) hemW 2419875..2421011 (-) 1137 WP_060815921.1 radical SAM family heme chaperone HemW -
  CEP66_RS11795 (CEP66_11735) - 2421004..2421597 (-) 594 WP_058668080.1 XTP/dITP diphosphatase -
  CEP66_RS11800 (CEP66_11740) yggU 2421605..2421895 (-) 291 WP_012135069.1 DUF167 family protein YggU -
  CEP66_RS11805 (CEP66_11745) - 2421892..2422458 (-) 567 WP_012135068.1 YggT family protein -
  CEP66_RS11810 (CEP66_11750) - 2422477..2423181 (-) 705 WP_058668079.1 YggS family pyridoxal phosphate-dependent enzyme -
  CEP66_RS11815 (CEP66_11755) pilT 2423199..2424179 (+) 981 WP_058668078.1 type IV pilus twitching motility protein PilT Machinery gene
  CEP66_RS11820 (CEP66_11760) ruvX 2424290..2424706 (-) 417 WP_058668077.1 Holliday junction resolvase RuvX -
  CEP66_RS11825 (CEP66_11765) - 2424706..2425269 (-) 564 WP_012135063.1 YqgE/AlgH family protein -
  CEP66_RS11830 (CEP66_11770) gshB 2425446..2426393 (-) 948 WP_012135062.1 glutathione synthase -
  CEP66_RS11835 (CEP66_11775) rsmE 2426406..2427137 (-) 732 WP_049010798.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CEP66_RS11840 (CEP66_11780) endA 2427215..2427922 (-) 708 WP_012135060.1 deoxyribonuclease I -
  CEP66_RS11845 (CEP66_11785) - 2428017..2428514 (-) 498 WP_058668076.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35680.74 Da        Isoelectric Point: 6.8793

>NTDB_id=236411 CEP66_RS11815 WP_058668078.1 2423199..2424179(+) (pilT) [Citrobacter koseri strain FDAARGOS_287]
MDMEEIVALSVKHNVSDLHLCNAWPVRWRKRGKVEIAPFSSPNVEVLLSQWLNDAQRRAWQADGQLDFAVSLPGELRLRA
SAFHHEQGASLALRLLPTTCPQLDALQTPAALPALLGGENGLILVTGATGSGKSTTLAAMVEYLNQHLDGHILTLEDPIE
YRHTSQRCLIQQREVGVHCRSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLETDRQDGRVALFELLINTPAVGNLIREGKIHQLPGVIQTSQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=236411 CEP66_RS11815 WP_058668078.1 2423199..2424179(+) (pilT) [Citrobacter koseri strain FDAARGOS_287]
ATGGATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAACGCATGGCCTGTGCG
CTGGCGTAAACGGGGAAAAGTGGAAATAGCCCCGTTTAGCAGCCCGAATGTCGAGGTTTTGCTGTCACAATGGCTTAACG
ACGCGCAGCGCCGGGCGTGGCAGGCGGATGGTCAGCTCGATTTTGCGGTGTCGCTGCCGGGCGAACTGCGGTTACGCGCC
AGCGCTTTTCATCACGAGCAGGGCGCATCGTTGGCGCTGCGGTTGCTGCCCACTACGTGCCCGCAACTGGACGCGCTCCA
GACGCCTGCCGCGCTACCCGCGCTCCTTGGCGGCGAGAACGGACTGATTCTGGTGACCGGGGCGACGGGAAGCGGGAAAT
CCACCACGCTGGCGGCAATGGTGGAATACCTCAACCAGCATCTCGATGGTCATATTCTGACGCTGGAAGATCCTATTGAG
TACCGCCATACCAGCCAGCGCTGCCTGATTCAGCAGCGGGAGGTTGGCGTACACTGCCGCTCCTTTGCCGCTGGGTTACG
CGCTGCGCTGCGTGAAGACCCGGATGTGATTTTATTAGGCGAACTGCGCGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCGACGCTACATACGCGCGGCGCGGCACAGGCGGTAGAGCGGCTGGTTGACTCC
TTTCCGGCGCAGGAGAAAGATCCGGTACGCAGTCAACTTGCGGGCAGCCTGCGCGCCGTGTTGTCGCAGAAGCTGGAAAC
TGACAGGCAGGACGGACGCGTGGCGTTATTCGAACTGCTCATCAACACGCCAGCGGTTGGCAATCTTATTCGGGAGGGGA
AAATTCACCAGTTGCCCGGCGTTATTCAGACCAGCCAACAGGCGGGGATGCAGACGTTTGCCCAGAGTCTGCAACAGCGG
CAGGCGCAGGGCAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Neisseria meningitidis 8013

49.39

100

0.497
  pilT Neisseria gonorrhoeae MS11

49.085

100

0.494
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilU Pseudomonas stutzeri DSM 10701

40.909

100

0.414
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

38.58

99.387

0.383

Multiple sequence alignment