Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CEP87_RS09015 Genome accession   NZ_CP022043
Coordinates   2116099..2117151 (-) Length   350 a.a.
NCBI ID   WP_011512665.1    Uniprot ID   Q1QE26
Organism   Psychrobacter cryohalolentis strain FDAARGOS_308     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2111099..2122151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP87_RS08985 (CEP87_08990) - 2111228..2111746 (-) 519 WP_011513068.1 helix-turn-helix domain-containing protein -
  CEP87_RS08990 (CEP87_08995) - 2111972..2113114 (+) 1143 WP_011512660.1 hypothetical protein -
  CEP87_RS08995 (CEP87_09000) - 2113102..2113506 (+) 405 WP_011512661.1 RnfH family protein -
  CEP87_RS09000 (CEP87_09005) - 2113532..2113948 (-) 417 WP_011512662.1 outer membrane protein assembly factor BamE -
  CEP87_RS09005 (CEP87_09010) fur 2114273..2114707 (+) 435 WP_011512663.1 ferric iron uptake transcriptional regulator -
  CEP87_RS09010 (CEP87_09015) pilU 2114819..2115940 (-) 1122 WP_011512664.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CEP87_RS09015 (CEP87_09020) pilT 2116099..2117151 (-) 1053 WP_011512665.1 type IV pilus twitching motility protein PilT Machinery gene
  CEP87_RS09020 (CEP87_09025) - 2117471..2118229 (+) 759 WP_041752932.1 YggS family pyridoxal phosphate-dependent enzyme -
  CEP87_RS09025 (CEP87_09030) - 2118448..2119722 (-) 1275 WP_011512667.1 hemolysin family protein -
  CEP87_RS09030 (CEP87_09035) - 2119980..2121683 (-) 1704 WP_011512668.1 NAD+ synthase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39105.95 Da        Isoelectric Point: 6.6711

>NTDB_id=236092 CEP87_RS09015 WP_011512665.1 2116099..2117151(-) (pilT) [Psychrobacter cryohalolentis strain FDAARGOS_308]
MAEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPDMSHKVVHQLIYDIMNDRQRADFEEFFETDFSFEI
PNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHI
LTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
TIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMIT
LDQTLKNLVARGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=236092 CEP87_RS09015 WP_011512665.1 2116099..2117151(-) (pilT) [Psychrobacter cryohalolentis strain FDAARGOS_308]
ATGGCTGAACAAAATTTAAGCATTGAAGACTTGCTGCGTTTTACTGTCAAGCATAAGGCTTCAGATTTACATATTTCAGC
CGGTATGCCTGCGATGATTCGAGTTGATGGTGAGATGACTCGGATTAATATGCCAGATATGAGCCATAAGGTAGTTCATC
AGCTCATTTATGACATCATGAATGATAGACAACGTGCCGACTTTGAAGAGTTTTTTGAAACGGATTTTTCTTTTGAGATT
CCAAATCTAGCCCGTTTTCGGGTAAACGCATTTAATCAAAATCGCGGTGCAGGCGCCGTCTTTCGTACAATTCCTTCCAA
AGTTCTAACGATGGAAGATCTAGGTATGGGCGAAGTGTTTAAGCGTGTTTCAGACTTTAAACGTGGCGTGGTGTTGGTCA
CTGGTCCAACCGGCTCAGGTAAGTCGACCACGCTAGCTGCCATGATTGATTATATCAATGAAACCCGTAAAGAGCATATT
TTGACTATCGAAGACCCGATTGAATTTGTTCATGAGTCCAAAAAGAGTTTGATTAACCAGCGTGAAGTCCATCGCGATAC
CTTGGGGTTCGAGCCAGCATTGCGCTCAGCGTTACGTGAAGATCCGGACGTTATTCTGGTTGGTGAGCTACGAGATCTTG
AGACCATACGCTTAGCGCTCACTGCCGCTGAAACCGGTCACTTAGTTTTCGGCACTTTGCACACCAGTTCCGCTGCCAAA
ACCATTGACCGTGTTATTGACGTCTTCCCTGCTGCTGAAAAAGACATGATTCGAGCGATGCTATCAGAGTCACTACAAGC
CGTTATCTCGCAAACATTGTTACGTCGTCAAACAGGCGGGCGAATTGCCGCCCATGAGATTATGTTAGGTACGCCCGCTA
TTCGTAACTTAATACGAGAAAATAAAATCGCTCAAATGTACTCTTCTATCCAAACTGGTGCTGGTGATGGCATGATTACT
CTTGATCAAACTCTTAAAAATCTTGTAGCGAGAGGCACAATTAGTAAAGACGTTGCACGACCTTACGCTAAACAGCCTGA
AGCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1QE26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

79.42

98.571

0.783
  pilT Acinetobacter baumannii D1279779

79.42

98.571

0.783
  pilT Acinetobacter nosocomialis M2

79.13

98.571

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.907

98.286

0.766
  pilT Pseudomonas aeruginosa PAK

77.907

98.286

0.766
  pilT Acinetobacter baylyi ADP1

76.163

98.286

0.749
  pilT Legionella pneumophila strain Lp02

72.093

98.286

0.709
  pilT Legionella pneumophila strain ERS1305867

72.093

98.286

0.709
  pilT Neisseria meningitidis 8013

64.638

98.571

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

94

0.637
  pilT Vibrio cholerae strain A1552

67.781

94

0.637
  pilT Neisseria gonorrhoeae MS11

64.058

98.571

0.631
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.445

98.857

0.509
  pilU Acinetobacter baylyi ADP1

41.399

98

0.406
  pilU Vibrio cholerae strain A1552

43.344

92.286

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.003

96.857

0.397

Multiple sequence alignment