Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSPPH_RS02385 Genome accession   NC_005773
Coordinates   532037..533071 (+) Length   344 a.a.
NCBI ID   WP_004654396.1    Uniprot ID   A0A108WZN6
Organism   Pseudomonas savastanoi pv. phaseolicola 1448A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527037..538071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPPH_RS02355 (PSPPH_0463) rdgB 527251..527844 (-) 594 WP_004654385.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PSPPH_RS02360 (PSPPH_0464) metW 527878..528498 (-) 621 WP_004666458.1 methionine biosynthesis protein MetW -
  PSPPH_RS02365 (PSPPH_0465) metX 528506..529645 (-) 1140 WP_011167495.1 homoserine O-succinyltransferase MetX -
  PSPPH_RS02370 (PSPPH_0466) - 529838..530428 (-) 591 WP_004666457.1 YggT family protein -
  PSPPH_RS02375 (PSPPH_0467) proC 530454..531272 (-) 819 WP_019740614.1 pyrroline-5-carboxylate reductase -
  PSPPH_RS02380 (PSPPH_0468) - 531295..531981 (-) 687 WP_004666456.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSPPH_RS02385 (PSPPH_0469) pilT 532037..533071 (+) 1035 WP_004654396.1 type IV pilus twitching motility protein PilT Machinery gene
  PSPPH_RS02390 (PSPPH_0470) - 533166..533843 (-) 678 WP_019740415.1 C40 family peptidase -
  PSPPH_RS02395 (PSPPH_0471) - 534094..534498 (+) 405 WP_004666455.1 NINE protein -
  PSPPH_RS02400 (PSPPH_0472) - 535569..536840 (-) 1272 WP_004654402.1 dihydroorotase -
  PSPPH_RS02405 (PSPPH_0473) - 536837..537841 (-) 1005 WP_004666454.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37938.80 Da        Isoelectric Point: 7.0226

>NTDB_id=23203 PSPPH_RS02385 WP_004654396.1 532037..533071(+) (pilT) [Pseudomonas savastanoi pv. phaseolicola 1448A]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGSVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=23203 PSPPH_RS02385 WP_004654396.1 532037..533071(+) (pilT) [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCACCGAT
GATCCGCGTCGACGGCGATGTGCGGCGTATCAACCTGCCCCCGCTGGACGCCAAAGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGGCAGGATTTCGAGGAGCGTCTGGAAACCGACTTTTCCTTTGAAGTGCCGGGCGTTGCGCGT
TTTCGGGTCAACGCATTCAACCAGAATCGCGGCGCAGGCGCGGTGTTTCGTACCATTCCCTCGAAAATCCTGAGCATGGA
AGACTTGGGGATGGGCAGTGTGTTTCGGAAGATTACCGACGTGGCACGCGGCCTGATTCTGGTCACCGGGCCGACCGGCT
CAGGCAAGTCGACGACGCTGGCGGCGATGATCGACTACCTCAATTGCAACAAGCATCACCACATCCTGACCATCGAGGAC
CCGATCGAATTCGTTCATGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACACTGGGTTTCTCGGA
GGCGCTGCGCTCGGCGTTGCGTGAAGACCCTGACGTGATTCTGGTCGGCGAGATGCGTGACCTGGAGACCATTCGCCTGG
CGCTCACCGCGGCTGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCACGTCAGCAGCCAAGACCATCGACCGGATC
GTCGATGTATTTCCGGCTCAGGAAAAATCGATGATTCGCTCGATGCTCTCCGAATCGCTGCACGCCGTGGTTTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGCGGACGCGTGGCAGCGCATGAAATCATGATGGGCACCCCGGCCATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCTATCCAGACCGGCGGTTCGATGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTGAAGAAGGGGCTGATCACCCGCGAAAGCGCGCGCGAACGTGCCAAGGTGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A108WZN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

78.779

100

0.788
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

67.941

98.837

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392

Multiple sequence alignment