Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BJE04_RS13705 Genome accession   NC_005139
Coordinates   2927064..2928101 (-) Length   345 a.a.
NCBI ID   WP_011079462.1    Uniprot ID   A0A3Q0L3W1
Organism   Vibrio vulnificus YJ016     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2922064..2933101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJE04_RS13675 (VV2866) - 2922278..2922973 (+) 696 WP_011151190.1 endonuclease -
  BJE04_RS13680 (VV2867) rsmE 2923085..2923816 (+) 732 WP_011079467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BJE04_RS13685 (VV2868) gshB 2923831..2924784 (+) 954 WP_043877324.1 glutathione synthase -
  BJE04_RS13690 (VV2869) - 2924839..2925402 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  BJE04_RS13695 (VV2870) ruvX 2925424..2925846 (+) 423 WP_011151193.1 Holliday junction resolvase RuvX -
  BJE04_RS13700 (VV2872) pilU 2925939..2927045 (-) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BJE04_RS13705 (VV2874) pilT 2927064..2928101 (-) 1038 WP_011079462.1 type IV pilus twitching motility protein PilT Machinery gene
  BJE04_RS13710 (VV2873) - 2928126..2928836 (+) 711 WP_011151196.1 YggS family pyridoxal phosphate-dependent enzyme -
  BJE04_RS13715 (VV2875) proC 2928871..2929689 (+) 819 WP_011151197.1 pyrroline-5-carboxylate reductase -
  BJE04_RS13720 (VV2876) - 2929731..2930288 (+) 558 WP_011151198.1 YggT family protein -
  BJE04_RS13725 (VV2877) yggU 2930288..2930578 (+) 291 WP_011151199.1 DUF167 family protein YggU -
  BJE04_RS13730 (VV2878) - 2930621..2931052 (+) 432 WP_011151200.1 DUF4426 domain-containing protein -
  BJE04_RS13735 (VV2879) - 2931063..2931665 (+) 603 WP_011079456.1 XTP/dITP diphosphatase -
  BJE04_RS13740 (VV2880) hemW 2931675..2932844 (+) 1170 WP_011151201.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38515.16 Da        Isoelectric Point: 6.3696

>NTDB_id=23149 BJE04_RS13705 WP_011079462.1 2927064..2928101(-) (pilT) [Vibrio vulnificus YJ016]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTNIPTLEELECPQIFEKIAKCEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLIAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=23149 BJE04_RS13705 WP_011079462.1 2927064..2928101(-) (pilT) [Vibrio vulnificus YJ016]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGTTGGAAGTCGATTTTTCCTTTGAATTGCCTAACGTAGGTCGT
TTTCGTGTTAACGCCTTCAACCAATCTCGCGGCTGCTCTGCGGTGTTTCGTACCATTCCAACCAATATCCCTACCTTGGA
AGAGCTTGAGTGCCCGCAAATTTTTGAAAAAATTGCGAAGTGTGAAAAAGGCTTGGTGTTGGTCACCGGGCCGACAGGTT
CGGGTAAATCGACCACGTTAGCGGCGATGGTGGATTACATTAACCGCAACTACAATAAACACATCCTGACCATTGAAGAT
CCGATTGAATTTGTTCACCAAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGTGACACCCACAGTTTTAAAAA
TGCCCTGCGCAGTGCTTTGCGTGAGGACCCAGACGTTATCTTGGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTTACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCGGCAAAAACCATTGACCGTATT
ATTGATGTGTTCCCTGGCAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCACTGCGCGCCGTTATCGCCCAGAA
ACTGCTGAAACGCATTGGCGGCGGGCGAGTGGCGTGTCATGAAATCATGATGGCGACGCCTGCCATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAGATGTACTCCATTATCCAAACAGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATTGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.406

100

0.884
  pilT Vibrio cholerae strain A1552

88.406

100

0.884
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.477

94.783

0.687
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

68.58

95.942

0.658
  pilT Legionella pneumophila strain ERS1305867

68.58

95.942

0.658
  pilT Neisseria meningitidis 8013

66.066

96.522

0.638
  pilT Neisseria gonorrhoeae MS11

65.766

96.522

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

99.42

0.516
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment