Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B9H00_RS11350 Genome accession   NZ_CP021358
Coordinates   2494354..2495364 (+) Length   336 a.a.
NCBI ID   WP_086900757.1    Uniprot ID   A0A240UPY9
Organism   Kushneria marisflavi strain SW32     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2489354..2500364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9H00_RS11320 (B9H00_11310) - 2489728..2490135 (-) 408 WP_086900751.1 acyl-CoA thioesterase -
  B9H00_RS11325 (B9H00_11315) metW 2490208..2490798 (-) 591 WP_086900752.1 methionine biosynthesis protein MetW -
  B9H00_RS11330 (B9H00_11320) - 2490798..2491964 (-) 1167 WP_086900753.1 homoserine O-acetyltransferase -
  B9H00_RS11335 (B9H00_11325) - 2492105..2492686 (-) 582 WP_086900754.1 YggT family protein -
  B9H00_RS11340 (B9H00_11330) proC 2492732..2493559 (-) 828 WP_086900755.1 pyrroline-5-carboxylate reductase -
  B9H00_RS11345 (B9H00_11335) - 2493587..2494315 (-) 729 WP_086900756.1 YggS family pyridoxal phosphate-dependent enzyme -
  B9H00_RS11350 (B9H00_11340) pilT 2494354..2495364 (+) 1011 WP_086900757.1 type IV pilus twitching motility protein PilT Machinery gene
  B9H00_RS11355 (B9H00_11345) - 2495405..2496520 (-) 1116 WP_086900758.1 anhydro-N-acetylmuramic acid kinase -
  B9H00_RS11360 (B9H00_11350) tyrS 2496651..2497856 (+) 1206 WP_086900759.1 tyrosine--tRNA ligase -
  B9H00_RS17125 - 2498051..2498179 (+) 129 WP_257789414.1 hypothetical protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 36411.73 Da        Isoelectric Point: 6.5040

>NTDB_id=230144 B9H00_RS11350 WP_086900757.1 2494354..2495364(+) (pilT) [Kushneria marisflavi strain SW32]
MDITELLAFTAKQGASDLHLSAGLPAMMRVDGEIRRLNAPALEHEDVERLLMAVMSQPQQQNFREALELDFSIDVPGVSR
FRVNAFHQDRGAAGVFRTIPAHVPSLESLGLGDVFRRLASCPHGLVLVTGPTGSGKSTTLAAMVDHINGARAQHVLTIED
PIEFVHHSRRALINQREVQRDTHNVASALRSALREDPDVILVGEMRDLETMRLALTAAETGHLVLATLHTTSAARTVDRI
VDVFPGDEKAMVRAMLSESLQGVISQQLVRRIGGGRIAAHEILIATPAIRNLIREDKVAQIYSALQTGGGLGMQTLDAAM
ARLAAEGLILREEHGG

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=230144 B9H00_RS11350 WP_086900757.1 2494354..2495364(+) (pilT) [Kushneria marisflavi strain SW32]
ATGGATATTACCGAACTGCTGGCTTTTACGGCAAAACAGGGCGCCTCGGATCTGCATCTGTCGGCCGGTTTGCCTGCCAT
GATGCGCGTTGACGGCGAAATTCGTCGCCTCAACGCGCCAGCACTTGAACACGAGGACGTTGAGCGCCTTCTGATGGCGG
TCATGAGTCAGCCGCAGCAGCAGAATTTCAGGGAGGCACTGGAACTCGACTTTTCTATCGATGTCCCCGGCGTCTCGCGC
TTTCGTGTTAACGCCTTTCATCAGGACAGGGGCGCCGCTGGCGTTTTTCGTACCATTCCGGCTCATGTCCCCAGTCTTGA
GTCACTGGGGCTTGGAGACGTTTTTCGGCGTCTTGCCAGCTGTCCGCATGGGCTTGTGCTGGTGACGGGACCGACCGGCT
CGGGCAAGTCCACGACGCTGGCGGCCATGGTGGATCATATCAATGGGGCCCGAGCTCAGCACGTGCTGACCATCGAAGAC
CCTATCGAGTTTGTGCATCACTCCAGGCGTGCCCTCATTAACCAGCGCGAAGTGCAGCGCGACACGCATAATGTGGCCAG
CGCCCTGCGCTCTGCCCTGCGTGAGGATCCTGACGTCATTCTGGTCGGAGAAATGCGCGACCTGGAAACCATGCGGCTGG
CCCTGACAGCGGCCGAGACCGGCCATCTGGTATTGGCCACGCTTCACACTACCTCGGCGGCCAGAACGGTAGATCGTATC
GTGGATGTCTTTCCTGGAGATGAAAAGGCGATGGTGCGTGCCATGCTGTCCGAGTCGCTTCAAGGGGTGATCTCCCAGCA
GCTGGTCCGTCGTATCGGTGGGGGGAGAATTGCCGCCCACGAAATTCTGATCGCCACGCCTGCCATTCGAAATCTGATTC
GTGAAGACAAGGTGGCGCAGATTTATTCGGCGCTTCAGACCGGTGGCGGGCTGGGCATGCAGACCCTGGACGCGGCCATG
GCTCGACTGGCGGCCGAAGGATTGATCCTGCGTGAAGAGCATGGTGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240UPY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.386

98.81

0.705
  pilT Pseudomonas stutzeri DSM 10701

69.578

98.81

0.687
  pilT Acinetobacter baumannii D1279779

68.997

97.917

0.676
  pilT Acinetobacter baumannii strain A118

68.997

97.917

0.676
  pilT Acinetobacter nosocomialis M2

68.693

97.917

0.673
  pilT Acinetobacter baylyi ADP1

66.565

97.917

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.363

99.107

0.628
  pilT Vibrio cholerae strain A1552

63.363

99.107

0.628
  pilT Neisseria meningitidis 8013

61.862

99.107

0.613
  pilT Neisseria gonorrhoeae MS11

61.562

99.107

0.61
  pilT Legionella pneumophila strain Lp02

60.79

97.917

0.595
  pilT Legionella pneumophila strain ERS1305867

60.79

97.917

0.595
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.898

99.405

0.506
  pilU Pseudomonas stutzeri DSM 10701

42.216

99.405

0.42
  pilU Vibrio cholerae strain A1552

42.121

98.214

0.414
  pilU Acinetobacter baylyi ADP1

41.231

96.726

0.399

Multiple sequence alignment