Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CAP31_RS02425 Genome accession   NZ_CP021138
Coordinates   481224..482267 (-) Length   347 a.a.
NCBI ID   WP_087446080.1    Uniprot ID   A0A1Y0G623
Organism   Sulfuriferula sp. AH1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476224..487267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAP31_RS02395 (CAP31_02395) - 476905..477414 (+) 510 WP_087446074.1 peroxiredoxin -
  CAP31_RS02400 (CAP31_02400) - 477411..477956 (+) 546 WP_087446075.1 DinB family protein -
  CAP31_RS02405 (CAP31_02405) - 477946..478482 (-) 537 WP_087446076.1 peroxiredoxin -
  CAP31_RS02410 (CAP31_02410) - 478495..478911 (+) 417 WP_087446077.1 CopD family protein -
  CAP31_RS02415 (CAP31_02415) - 478908..480059 (+) 1152 WP_087446078.1 class I SAM-dependent RNA methyltransferase -
  CAP31_RS02420 (CAP31_02420) pilU 480092..481219 (-) 1128 WP_087446079.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CAP31_RS02425 (CAP31_02425) pilT 481224..482267 (-) 1044 WP_087446080.1 type IV pilus twitching motility protein PilT Machinery gene
  CAP31_RS02430 (CAP31_02430) - 482301..482990 (+) 690 WP_087446081.1 YggS family pyridoxal phosphate-dependent enzyme -
  CAP31_RS02435 (CAP31_02435) proC 482996..483802 (+) 807 WP_087448224.1 pyrroline-5-carboxylate reductase -
  CAP31_RS02440 (CAP31_02440) - 483820..484395 (+) 576 WP_087446082.1 YggT family protein -
  CAP31_RS02445 (CAP31_02445) - 484478..486397 (+) 1920 WP_157662630.1 dynamin family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38388.08 Da        Isoelectric Point: 6.6651

>NTDB_id=228500 CAP31_RS02425 WP_087446080.1 481224..482267(-) (pilT) [Sulfuriferula sp. AH1]
MEISELLAFAVKNKASDLHLSAGLPPMIRVHGDIRRINLPDMNHQTVHDMVYDIMNDGQRKIYEETLEVDFSFEVPTLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNAPKIFKDIADTPRGIVLVTGPTGSGKSTTLAAMVDYVNENEYGHILTVED
PIEFVHQSKKCVINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESIRAVISQTLLKNKEGTGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LQDLVKRNVISVGEARSRAANKDMFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=228500 CAP31_RS02425 WP_087446080.1 481224..482267(-) (pilT) [Sulfuriferula sp. AH1]
ATGGAAATATCCGAACTGCTGGCTTTCGCAGTCAAAAACAAGGCATCCGACTTGCATCTCTCGGCCGGCTTGCCGCCGAT
GATACGCGTCCACGGCGATATCCGCCGCATCAATCTGCCGGACATGAATCATCAAACCGTACACGACATGGTGTACGACA
TTATGAACGATGGCCAACGCAAAATATACGAAGAAACCCTGGAAGTGGACTTTTCGTTCGAGGTCCCCACGCTGGCACGT
TTCCGCGTCAATGCGTTCAACCAGAATCGCGGCGCAGGCGCGGTATTCCGTACCATTCCCAGCAAGGTACTGACGCTGGA
AGAGCTGAATGCGCCCAAGATATTCAAGGACATCGCCGATACCCCTCGCGGCATTGTACTCGTTACCGGACCTACCGGCT
CGGGCAAATCGACGACACTTGCGGCCATGGTGGATTACGTCAATGAAAACGAATATGGCCACATCCTCACCGTCGAAGAC
CCGATCGAATTCGTACATCAAAGCAAAAAATGCGTGATCAACCAGCGCGAAGTCGGGCCGCATACCTTGTCGTTTGCCAA
CGCACTGCGCTCGGCGCTGCGTGAAGATCCGGACGTCATCCTCGTCGGCGAAATGCGTGACCTTGAAACCATCCGCCTTG
CCCTTACCGCGGCGGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCAGCCAAGACCATAGACCGGATC
GTGGACGTTTTTCCTGCAGCCGAAAAGGAGATGGTGCGTTCCATGCTGTCCGAATCCATCCGTGCGGTCATTTCGCAAAC
GCTGCTCAAGAACAAGGAAGGCACAGGCCGCGTGGCCGCTCATGAAATCATGATAGGCACACCTGCAATTCGCAACCTGA
TCCGCGAAAACAAGATCGCCCAGATGTATTCCTCCATCCAGACCGGCCAGAATGTCGGCATGCAGACACTGGATCAATGT
TTGCAGGATTTGGTTAAACGCAACGTCATTTCCGTCGGCGAAGCCCGTTCACGCGCCGCTAACAAAGACATGTTCCTGGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0G623

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.324

99.712

0.781
  pilT Acinetobacter baumannii D1279779

78.324

99.712

0.781
  pilT Acinetobacter baumannii strain A118

78.324

99.712

0.781
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Neisseria meningitidis 8013

73.178

98.847

0.723
  pilT Neisseria gonorrhoeae MS11

72.886

98.847

0.72
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

40.294

97.983

0.395

Multiple sequence alignment