Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B9W14_RS05255 Genome accession   NZ_CP020953
Coordinates   1122658..1123713 (+) Length   351 a.a.
NCBI ID   WP_084572230.1    Uniprot ID   A0A2U8DN96
Organism   Clostridium drakei strain SL1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1117658..1128713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9W14_RS05235 (B9W14_05180) - 1118006..1119226 (+) 1221 WP_032077724.1 U32 family peptidase -
  B9W14_RS05240 (B9W14_05185) udk 1119254..1119874 (+) 621 WP_032077723.1 uridine kinase -
  B9W14_RS05245 (B9W14_05190) - 1119941..1121599 (+) 1659 WP_032077722.1 penicillin-binding transpeptidase domain-containing protein -
  B9W14_RS05250 (B9W14_05195) sigK 1121733..1122434 (+) 702 WP_029161424.1 RNA polymerase sporulation sigma factor SigK -
  B9W14_RS05255 (B9W14_05200) pilT 1122658..1123713 (+) 1056 WP_084572230.1 type IV pilus twitching motility protein PilT Machinery gene
  B9W14_RS26025 - 1123753..1123911 (-) 159 WP_200880557.1 hypothetical protein -
  B9W14_RS05260 (B9W14_05205) - 1124335..1125609 (+) 1275 WP_032077720.1 glycosyltransferase family 2 protein -
  B9W14_RS05265 (B9W14_05210) ftsA 1125783..1127054 (+) 1272 WP_032077719.1 cell division protein FtsA -
  B9W14_RS05270 (B9W14_05215) ftsZ 1127070..1128200 (+) 1131 WP_032077718.1 cell division protein FtsZ -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38802.76 Da        Isoelectric Point: 6.6991

>NTDB_id=227336 B9W14_RS05255 WP_084572230.1 1122658..1123713(+) (pilT) [Clostridium drakei strain SL1]
MISLKELLEITIKNNASDLHLTVGVPPIIRIDGELSNVNNEKLQPADTESYSREILNEDYEEYTKKGEMDTSFSVSGLGR
FRVNLFKQRGSCSLAIRVVGLKIPSIGDLNFPSSIKEITNNKRGLVLVTGPTGSGKSTTLAAMINEINCTRAEHIITLED
PIEYLHKHNKSIINQREIGKDSLSYHNALKAVLREDPDVILVGEMRDLETISIALIAAETGHLVFSTLHTIGASKTIDRI
VDVFPPYQQQQVKTQLAAVLKGIISQQLLPKVDGNGRIASLEIMMATAAIQNMIREGKTHQIESSIQTGAKYGMKTMDMS
LAELYKKGMISYECALSYAMDRDILSRIISL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=227336 B9W14_RS05255 WP_084572230.1 1122658..1123713(+) (pilT) [Clostridium drakei strain SL1]
GTGATATCACTAAAGGAATTATTGGAAATAACCATTAAAAATAATGCATCTGATTTACATTTAACTGTAGGAGTTCCTCC
AATAATAAGAATAGATGGAGAATTGTCAAATGTTAATAATGAAAAACTTCAACCAGCAGATACGGAAAGCTATAGTAGAG
AAATTTTAAATGAAGACTACGAAGAGTATACTAAAAAAGGTGAAATGGATACTTCTTTTTCCGTGTCAGGATTAGGAAGA
TTTAGAGTTAATTTGTTCAAACAAAGAGGAAGCTGTTCATTAGCTATAAGGGTTGTAGGATTAAAGATACCTTCAATAGG
TGATTTGAATTTTCCATCTTCAATAAAAGAAATTACAAATAACAAAAGAGGTCTTGTATTAGTTACAGGTCCTACTGGGT
CAGGGAAAAGTACTACATTAGCAGCAATGATAAATGAAATAAATTGTACTAGAGCAGAACATATTATTACTCTTGAAGAT
CCCATTGAATATTTACATAAACATAATAAATCTATAATAAATCAAAGAGAAATAGGAAAAGATAGCTTGAGTTATCATAA
TGCTTTAAAAGCGGTTTTAAGGGAAGATCCAGATGTTATTCTTGTGGGAGAAATGAGGGACTTAGAAACTATTTCTATAG
CACTTATAGCTGCTGAAACAGGACATTTGGTATTTTCTACATTACATACAATAGGTGCATCAAAAACTATTGATAGAATA
GTTGATGTATTTCCTCCATATCAACAGCAGCAAGTAAAAACACAGTTAGCAGCAGTGCTTAAAGGAATTATATCACAACA
ATTATTGCCCAAAGTTGATGGTAATGGAAGAATAGCAAGCTTAGAAATAATGATGGCAACTGCAGCTATCCAAAATATGA
TAAGAGAAGGAAAAACTCATCAAATAGAATCTTCCATACAAACAGGAGCTAAATATGGTATGAAAACTATGGATATGTCT
TTAGCTGAGTTATATAAAAAAGGAATGATATCATATGAATGTGCTTTAAGTTATGCTATGGATAGGGATATACTAAGCAG
AATAATATCATTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8DN96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.877

97.436

0.496
  pilT Acinetobacter nosocomialis M2

50.585

97.436

0.493
  pilT Acinetobacter baumannii D1279779

50.585

97.436

0.493
  pilT Acinetobacter baumannii strain A118

50.585

97.436

0.493
  pilT Legionella pneumophila strain Lp02

50.145

98.291

0.493
  pilT Legionella pneumophila strain ERS1305867

50.145

98.291

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.424

94.017

0.493
  pilT Vibrio cholerae strain A1552

52.424

94.017

0.493
  pilT Neisseria meningitidis 8013

49.28

98.86

0.487
  pilT Pseudomonas stutzeri DSM 10701

50.296

96.296

0.484
  pilT Neisseria gonorrhoeae MS11

48.844

98.575

0.481
  pilT Pseudomonas aeruginosa PAK

49.415

97.436

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.276

99.145

0.479
  pilU Pseudomonas stutzeri DSM 10701

41.026

100

0.41
  pilU Acinetobacter baylyi ADP1

40.525

97.721

0.396
  pilU Vibrio cholerae strain A1552

39.349

96.296

0.379

Multiple sequence alignment