Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B9G79_RS00460 Genome accession   NZ_CP020946
Coordinates   98540..99640 (-) Length   366 a.a.
NCBI ID   WP_088563811.1    Uniprot ID   A0A1Z3N3T8
Organism   Bdellovibrio bacteriovorus strain SSB218315     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93540..104640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9G79_RS00435 (B9G79_00435) - 93903..95132 (-) 1230 WP_088563806.1 S8 family peptidase -
  B9G79_RS00440 (B9G79_00440) - 95277..96065 (-) 789 WP_420042860.1 purine-nucleoside phosphorylase -
  B9G79_RS00445 (B9G79_00445) - 96120..97439 (-) 1320 WP_088563808.1 thymidine phosphorylase -
  B9G79_RS00450 (B9G79_00450) deoC 97439..98101 (-) 663 WP_088563809.1 deoxyribose-phosphate aldolase -
  B9G79_RS00455 (B9G79_00455) - 98161..98538 (+) 378 WP_232468932.1 hypothetical protein -
  B9G79_RS00460 (B9G79_00460) pilT 98540..99640 (-) 1101 WP_088563811.1 type IV pilus twitching motility protein PilT Machinery gene
  B9G79_RS00465 (B9G79_00465) - 99788..101305 (+) 1518 WP_088563812.1 Rne/Rng family ribonuclease -
  B9G79_RS00470 (B9G79_00470) - 101420..103021 (+) 1602 WP_088563813.1 hypothetical protein -
  B9G79_RS18555 (B9G79_00475) rplU 103229..103864 (+) 636 WP_088563814.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 39794.96 Da        Isoelectric Point: 7.9844

>NTDB_id=227321 B9G79_RS00460 WP_088563811.1 98540..99640(-) (pilT) [Bdellovibrio bacteriovorus strain SSB218315]
MATIDELFKLMVEQGASDLHITSGAAPYLRLHGNMVPLNYRELTNQDVQGLLFEILSEKQKKAFVEKWELDFAYTLSGIG
RFRCNIFMQRKGLGAVMRIIPEKIKTAQELGLPPAVMDMIDCDRGLILVTGPTGSGKSTTLAAMIHQINMTREAHIITVE
DPIEFVHPNLKSLVNQREVGSHTKSFANALKAALREDPDILLVGELRDLETISLALTAAETGHIVFGTLHTNSAAKTIDR
IIDVFPAGQQQQIRTMLAESLRGVVAQTLFSRADGQGRVAAYEIMRNTKAISNLVREGKVHQIPSAMQTGATQGMVLFEK
YIEDLVRKGKVSAADAKTFLGQAGGGDTSVQPGATMAGGTPRTKVG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=227321 B9G79_RS00460 WP_088563811.1 98540..99640(-) (pilT) [Bdellovibrio bacteriovorus strain SSB218315]
ATGGCAACAATTGATGAATTGTTCAAACTCATGGTGGAACAAGGTGCCTCCGACTTGCATATCACAAGTGGAGCCGCCCC
TTATTTGCGCCTGCATGGAAACATGGTTCCATTGAACTATCGCGAGCTCACAAATCAGGACGTACAGGGACTGCTGTTTG
AGATTCTTTCTGAAAAGCAGAAAAAAGCTTTCGTGGAAAAATGGGAACTGGATTTCGCCTACACTTTGTCAGGTATCGGG
CGTTTCCGTTGCAATATCTTTATGCAGCGTAAAGGCCTTGGCGCCGTTATGCGTATCATCCCGGAAAAAATCAAAACCGC
CCAAGAACTGGGATTGCCTCCGGCCGTGATGGACATGATCGATTGCGATCGTGGTCTGATTCTTGTCACCGGTCCGACAG
GTTCTGGTAAGTCCACAACTCTGGCGGCGATGATTCACCAAATTAACATGACCCGTGAAGCCCACATCATCACGGTGGAA
GACCCGATCGAGTTCGTTCACCCGAATTTGAAATCTTTGGTCAATCAGCGTGAGGTGGGAAGCCACACCAAAAGTTTCGC
CAACGCCCTGAAAGCGGCCCTGCGTGAAGACCCGGATATTCTGCTGGTGGGTGAGCTTCGTGACCTAGAAACAATTTCTT
TGGCACTGACTGCTGCCGAAACAGGTCATATCGTTTTCGGAACCCTGCACACCAACAGTGCGGCGAAAACCATCGATCGT
ATCATTGACGTATTCCCTGCCGGTCAACAGCAACAGATCCGAACGATGCTGGCTGAATCCCTGCGCGGAGTTGTCGCTCA
GACCCTGTTCTCCCGCGCGGACGGACAAGGTCGTGTGGCGGCTTACGAGATCATGAGAAACACCAAAGCCATCTCGAACT
TGGTTCGTGAAGGTAAAGTCCACCAGATCCCATCTGCCATGCAGACCGGTGCCACTCAAGGTATGGTTCTGTTTGAAAAG
TACATTGAGGATCTGGTTCGTAAGGGTAAAGTTTCCGCAGCCGATGCCAAGACCTTCCTGGGTCAGGCCGGTGGTGGCGA
CACCAGCGTACAGCCAGGGGCGACCATGGCTGGCGGCACCCCACGCACCAAAGTGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N3T8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.515

99.18

0.511
  pilT Acinetobacter nosocomialis M2

54.627

91.53

0.5
  pilT Acinetobacter baumannii D1279779

54.627

91.53

0.5
  pilT Acinetobacter baumannii strain A118

54.627

91.53

0.5
  pilT Acinetobacter baylyi ADP1

54.491

91.257

0.497
  pilT Pseudomonas aeruginosa PAK

54.491

91.257

0.497
  pilT Vibrio cholerae strain A1552

54.03

91.53

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.03

91.53

0.495
  pilT Pseudomonas stutzeri DSM 10701

53.892

91.257

0.492
  pilT Legionella pneumophila strain ERS1305867

54.407

89.891

0.489
  pilT Legionella pneumophila strain Lp02

54.407

89.891

0.489
  pilT Neisseria gonorrhoeae MS11

50.599

91.257

0.462
  pilT Neisseria meningitidis 8013

50.599

91.257

0.462
  pilU Pseudomonas stutzeri DSM 10701

41.84

92.077

0.385
  pilU Acinetobacter baylyi ADP1

41.018

91.257

0.374
  pilU Vibrio cholerae strain A1552

41.104

89.071

0.366

Multiple sequence alignment