Detailed information NTG0224677

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: B7L39_RS00025
• Genome accession: NZ_CP020592
• Coordinates: 2445..3482 (-)
• Length: 345 a.a.
• NCBI ID: WP_000355489.1
• Uniprot ID: A0A009PTR1
• Organism: Acinetobacter baumannii strain USA2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1..8482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L39_RS00010 (B7L39_00010)	-	282..680 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
B7L39_RS00015 (B7L39_00015)	fur	792..1229 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
B7L39_RS00020 (B7L39_00020)	pilU	1299..2417 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B7L39_RS00025 (B7L39_00025)	pilT	2445..3482 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
B7L39_RS00030 (B7L39_00030)	-	3609..4301 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
B7L39_RS00035 (B7L39_00035)	-	4503..8099 (-)	3597	WP_000698785.1	AAA family ATPase	-

Sequence

Protein

• Length: 345 a.a.
• Molecular weight: 38312.92 Da
• Isoelectric Point: 6.7271

>NTDB_id=224677 B7L39_RS00025 WP_000355489.1 2445..3482(-) (pilT) [Acinetobacter baumannii strain USA2]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide

• Length: 1038 bp

>NTDB_id=224677 B7L39_RS00025 WP_000355489.1 2445..3482(-) (pilT) [Acinetobacter baumannii strain USA2]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCCGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CAGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTACACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCCATTCAAACTGGTGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGCCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-271)

3D structure

• AlphaFold DB: A0A009PTR1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Acinetobacter baumannii strain A118	100	100	1
pilT	Acinetobacter baumannii D1279779	100	100	1
pilT	Acinetobacter nosocomialis M2	99.71	100	0.997
pilT	Acinetobacter baylyi ADP1	88.953	99.71	0.887
pilT	Pseudomonas aeruginosa PAK	84.302	99.71	0.841
pilT	Pseudomonas stutzeri DSM 10701	84.012	99.71	0.838
pilT	Legionella pneumophila strain Lp02	75.581	99.71	0.754
pilT	Legionella pneumophila strain ERS1305867	75.581	99.71	0.754
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	71.726	97.391	0.699
pilT	Vibrio cholerae strain A1552	71.726	97.391	0.699
pilT	Neisseria meningitidis 8013	68.116	100	0.681
pilT	Neisseria gonorrhoeae MS11	67.826	100	0.678
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	52.174	100	0.522
pilU	Vibrio cholerae strain A1552	41.593	98.261	0.409
pilU	Pseudomonas stutzeri DSM 10701	40.597	97.101	0.394
pilU	Acinetobacter baylyi ADP1	38.728	100	0.388