Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM681_RS21375 Genome accession   NZ_CP020503
Coordinates   4424175..4425197 (-) Length   340 a.a.
NCBI ID   WP_048234667.1    Uniprot ID   A0AAW6XAW6
Organism   Serratia marcescens strain 95     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4419175..4430197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM681_RS21345 (AM681_21245) - 4419974..4420486 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  AM681_RS21350 (AM681_21250) endA 4420588..4421283 (+) 696 WP_025304243.1 deoxyribonuclease I -
  AM681_RS21355 (AM681_21255) rsmE 4421353..4422084 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM681_RS21360 (AM681_21260) gshB 4422095..4423045 (+) 951 WP_048234666.1 glutathione synthase -
  AM681_RS21365 (AM681_21265) - 4423193..4423756 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  AM681_RS21370 (AM681_21270) ruvX 4423756..4424178 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AM681_RS21375 (AM681_21275) pilT 4424175..4425197 (-) 1023 WP_048234667.1 type IV pilus twitching motility protein PilT Machinery gene
  AM681_RS21380 (AM681_21280) - 4425218..4425925 (+) 708 WP_048234668.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM681_RS21385 (AM681_21285) proC 4425945..4426766 (+) 822 WP_048234669.1 pyrroline-5-carboxylate reductase -
  AM681_RS21390 (AM681_21290) - 4426798..4427352 (+) 555 WP_033635944.1 YggT family protein -
  AM681_RS21395 (AM681_21295) yggU 4427349..4427639 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  AM681_RS21400 (AM681_21300) - 4427657..4428250 (+) 594 WP_038879287.1 XTP/dITP diphosphatase -
  AM681_RS21405 (AM681_21305) hemW 4428243..4429385 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  AM681_RS21410 (AM681_21310) - 4429421..4429669 (-) 249 WP_038879292.1 DUF2442 domain-containing protein -
  AM681_RS21415 (AM681_21315) - 4429653..4429895 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36425.69 Da        Isoelectric Point: 7.2888

>NTDB_id=223813 AM681_RS21375 WP_048234667.1 4424175..4425197(-) (pilT) [Serratia marcescens strain 95]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAVPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGMSLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLSLTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=223813 AM681_RS21375 WP_048234667.1 4424175..4425197(-) (pilT) [Serratia marcescens strain 95]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGTGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGTGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAGGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGTCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGATGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGC
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

49.849

97.353

0.485
  pilT Neisseria gonorrhoeae MS11

49.547

97.353

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.071

99.118

0.476
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365

Multiple sequence alignment