Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AS657_RS20485 Genome accession   NZ_CP020501
Coordinates   4298208..4299230 (-) Length   340 a.a.
NCBI ID   WP_089196265.1    Uniprot ID   -
Organism   Serratia marcescens strain BWH-23     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4293208..4304230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS657_RS20455 (AS657_20405) - 4294007..4294519 (+) 513 WP_148123755.1 SprT family zinc-dependent metalloprotease -
  AS657_RS20460 (AS657_20410) endA 4294621..4295316 (+) 696 WP_043141331.1 deoxyribonuclease I -
  AS657_RS20465 (AS657_20415) rsmE 4295386..4296117 (+) 732 WP_049204216.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AS657_RS20470 (AS657_20420) gshB 4296128..4297078 (+) 951 WP_033651325.1 glutathione synthase -
  AS657_RS20475 (AS657_20425) - 4297226..4297789 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  AS657_RS20480 (AS657_20430) ruvX 4297789..4298211 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  AS657_RS20485 (AS657_20435) pilT 4298208..4299230 (-) 1023 WP_089196265.1 type IV pilus twitching motility protein PilT Machinery gene
  AS657_RS20490 (AS657_20440) - 4299251..4299958 (+) 708 WP_025304248.1 YggS family pyridoxal phosphate-dependent enzyme -
  AS657_RS20495 (AS657_20445) proC 4299978..4300799 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  AS657_RS20500 (AS657_20450) - 4300831..4301385 (+) 555 WP_025304250.1 YggT family protein -
  AS657_RS20505 (AS657_20455) yggU 4301382..4301672 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  AS657_RS20510 (AS657_20460) - 4301690..4302283 (+) 594 WP_043141349.1 XTP/dITP diphosphatase -
  AS657_RS20515 (AS657_20465) hemW 4302276..4303418 (+) 1143 WP_148123756.1 radical SAM family heme chaperone HemW -
  AS657_RS20520 (AS657_20470) - 4303456..4303890 (-) 435 WP_148123757.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36473.69 Da        Isoelectric Point: 7.0670

>NTDB_id=223790 AS657_RS20485 WP_089196265.1 4298208..4299230(-) (pilT) [Serratia marcescens strain BWH-23]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGMSLALRRIAGQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=223790 AS657_RS20485 WP_089196265.1 4298208..4299230(-) (pilT) [Serratia marcescens strain BWH-23]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCACAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGTGGAGAG
CGGCTGCGTGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGTCGCCCTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATTAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.851

98.529

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

40.184

95.882

0.385
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment