Detailed information
Bioinformatically predicted
Overview
| Name | pilT | Type | Machinery gene |
| Locus tag | B5S50_RS17590 | Genome accession | NZ_CP020473 |
| Coordinates | 3748798..3749853 (+) | Length | 351 a.a. |
| NCBI ID | WP_065077504.1 | Uniprot ID | A0A1A6AXQ6 |
| Organism | Clostridium sp. 001 | ||
| Function | type IV pilus retraction (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 3708225..3748524 | 3748798..3749853 | flank | 274 |
Gene organization within MGE regions
Location: 3708225..3749853
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B5S50_RS17310 (B5S50_17135) | - | 3708225..3708650 (+) | 426 | Protein_3399 | sigma-70 family RNA polymerase sigma factor | - |
| B5S50_RS17315 (B5S50_17140) | - | 3708673..3710178 (-) | 1506 | WP_217302412.1 | recombinase family protein | - |
| B5S50_RS17320 (B5S50_17145) | - | 3710193..3710561 (-) | 369 | WP_217302413.1 | helix-turn-helix transcriptional regulator | - |
| B5S50_RS17325 (B5S50_17150) | - | 3710805..3711008 (+) | 204 | WP_217302414.1 | helix-turn-helix domain-containing protein | - |
| B5S50_RS21860 | - | 3711001..3711132 (+) | 132 | WP_302180671.1 | hypothetical protein | - |
| B5S50_RS21865 | - | 3711151..3711273 (+) | 123 | WP_302180672.1 | hypothetical protein | - |
| B5S50_RS17330 (B5S50_17155) | - | 3711398..3711646 (+) | 249 | WP_217302415.1 | hypothetical protein | - |
| B5S50_RS17335 (B5S50_17160) | - | 3711875..3712612 (+) | 738 | WP_217302416.1 | hypothetical protein | - |
| B5S50_RS17340 (B5S50_17165) | - | 3712672..3713061 (+) | 390 | WP_217302417.1 | VRR-NUC domain-containing protein | - |
| B5S50_RS17345 (B5S50_17170) | - | 3713058..3713573 (+) | 516 | WP_217302418.1 | ParA family protein | - |
| B5S50_RS17350 (B5S50_17175) | - | 3713685..3714809 (+) | 1125 | WP_217302419.1 | IS4 family transposase | - |
| B5S50_RS17355 (B5S50_17180) | - | 3715086..3715292 (+) | 207 | WP_217302420.1 | ParA family protein | - |
| B5S50_RS17360 (B5S50_17185) | - | 3715320..3716354 (+) | 1035 | WP_217302421.1 | DUF3102 domain-containing protein | - |
| B5S50_RS17365 (B5S50_17190) | - | 3716355..3716552 (+) | 198 | WP_217302422.1 | hypothetical protein | - |
| B5S50_RS17370 (B5S50_17195) | - | 3716530..3716709 (+) | 180 | WP_063600489.1 | hypothetical protein | - |
| B5S50_RS17375 | - | 3716758..3716901 (+) | 144 | WP_156496363.1 | hypothetical protein | - |
| B5S50_RS17380 (B5S50_17200) | - | 3716945..3717418 (+) | 474 | WP_217302423.1 | hypothetical protein | - |
| B5S50_RS17385 (B5S50_17205) | - | 3717439..3718020 (+) | 582 | WP_217302424.1 | hypothetical protein | - |
| B5S50_RS17390 (B5S50_17210) | - | 3718056..3718517 (+) | 462 | WP_063600486.1 | ASCH domain-containing protein | - |
| B5S50_RS17395 (B5S50_17215) | - | 3718535..3718867 (+) | 333 | WP_063600485.1 | hypothetical protein | - |
| B5S50_RS17400 (B5S50_17220) | - | 3718895..3719344 (+) | 450 | WP_063600484.1 | hypothetical protein | - |
| B5S50_RS17405 (B5S50_17225) | - | 3719518..3719838 (+) | 321 | WP_063600483.1 | HNH endonuclease | - |
| B5S50_RS17410 (B5S50_17235) | - | 3720135..3720443 (+) | 309 | WP_063600481.1 | P27 family phage terminase small subunit | - |
| B5S50_RS17415 (B5S50_17240) | - | 3720433..3722094 (+) | 1662 | WP_217302426.1 | terminase TerL endonuclease subunit | - |
| B5S50_RS17420 (B5S50_17245) | - | 3722111..3722443 (+) | 333 | WP_217302427.1 | hypothetical protein | - |
| B5S50_RS17425 (B5S50_17250) | - | 3722493..3723641 (+) | 1149 | WP_217302428.1 | phage portal protein | - |
| B5S50_RS17430 (B5S50_17255) | - | 3723641..3724381 (+) | 741 | WP_217302429.1 | head maturation protease, ClpP-related | - |
| B5S50_RS17435 (B5S50_17260) | - | 3724383..3725579 (+) | 1197 | WP_254306618.1 | phage major capsid protein | - |
| B5S50_RS17440 | - | 3725594..3726166 (+) | 573 | WP_063600477.1 | hypothetical protein | - |
| B5S50_RS17445 (B5S50_17270) | - | 3726171..3726488 (+) | 318 | WP_063600476.1 | hypothetical protein | - |
| B5S50_RS17450 (B5S50_17275) | - | 3726494..3726883 (+) | 390 | WP_063600475.1 | HK97-gp10 family putative phage morphogenesis protein | - |
| B5S50_RS17455 (B5S50_17280) | - | 3726880..3727200 (+) | 321 | WP_063600474.1 | hypothetical protein | - |
| B5S50_RS17460 (B5S50_17285) | - | 3727268..3728140 (+) | 873 | WP_063600473.1 | major tail protein | - |
| B5S50_RS17465 (B5S50_17290) | - | 3728218..3728550 (+) | 333 | WP_082853501.1 | hypothetical protein | - |
| B5S50_RS17470 (B5S50_17295) | - | 3728860..3729336 (+) | 477 | WP_217302430.1 | DNA-binding protein | - |
| B5S50_RS17475 (B5S50_17300) | - | 3729409..3735837 (+) | 6429 | WP_217302431.1 | phage tail tape measure protein | - |
| B5S50_RS17480 (B5S50_17305) | - | 3735840..3736553 (+) | 714 | WP_217302432.1 | distal tail protein Dit | - |
| B5S50_RS17485 (B5S50_17310) | - | 3736553..3738328 (+) | 1776 | WP_217302433.1 | phage tail protein | - |
| B5S50_RS17490 (B5S50_17315) | - | 3738318..3739232 (+) | 915 | WP_217302434.1 | hypothetical protein | - |
| B5S50_RS17495 (B5S50_17320) | - | 3739233..3739448 (+) | 216 | WP_217302435.1 | hypothetical protein | - |
| B5S50_RS17500 (B5S50_17325) | - | 3739462..3739965 (+) | 504 | WP_063600466.1 | collagen-like protein | - |
| B5S50_RS17505 (B5S50_17330) | - | 3739978..3740796 (+) | 819 | WP_217302436.1 | hypothetical protein | - |
| B5S50_RS17510 (B5S50_17335) | - | 3740809..3741090 (+) | 282 | WP_217302437.1 | hypothetical protein | - |
| B5S50_RS17515 | - | 3741091..3741240 (+) | 150 | WP_217302438.1 | hypothetical protein | - |
| B5S50_RS17520 (B5S50_17340) | - | 3741314..3741568 (+) | 255 | WP_217302439.1 | BhlA/UviB family holin-like peptide | - |
| B5S50_RS17525 (B5S50_17345) | - | 3741584..3742345 (+) | 762 | WP_217302440.1 | N-acetylmuramoyl-L-alanine amidase | - |
| B5S50_RS17530 (B5S50_17350) | - | 3742458..3742922 (+) | 465 | WP_217302441.1 | hypothetical protein | - |
| B5S50_RS17535 (B5S50_17355) | - | 3743014..3743769 (+) | 756 | WP_217302442.1 | Dam family site-specific DNA-(adenine-N6)-methyltransferase | - |
| B5S50_RS17540 | - | 3743932..3744069 (+) | 138 | WP_217302443.1 | hypothetical protein | - |
| B5S50_RS17545 (B5S50_17360) | - | 3744264..3744476 (+) | 213 | WP_217302444.1 | helix-turn-helix domain-containing protein | - |
| B5S50_RS17550 (B5S50_17365) | - | 3744799..3744981 (+) | 183 | WP_217302445.1 | hypothetical protein | - |
| B5S50_RS17555 (B5S50_17370) | - | 3745106..3745777 (+) | 672 | WP_217302446.1 | hypothetical protein | - |
| B5S50_RS17560 (B5S50_17375) | - | 3745791..3746051 (+) | 261 | WP_217302447.1 | hypothetical protein | - |
| B5S50_RS17565 (B5S50_17380) | - | 3746052..3746948 (+) | 897 | WP_217302448.1 | ParM/StbA family protein | - |
| B5S50_RS17570 (B5S50_17385) | - | 3746929..3747258 (+) | 330 | WP_217302449.1 | hypothetical protein | - |
| B5S50_RS17575 | - | 3747293..3747442 (-) | 150 | WP_217302450.1 | hypothetical protein | - |
| B5S50_RS21970 (B5S50_17390) | - | 3747505..3747735 (-) | 231 | WP_217302451.1 | helix-turn-helix transcriptional regulator | - |
| B5S50_RS21870 | - | 3748051..3748176 (+) | 126 | WP_302180673.1 | hypothetical protein | - |
| B5S50_RS17585 (B5S50_17395) | - | 3748237..3748524 (+) | 288 | Protein_3457 | sigma-70 family RNA polymerase sigma factor | - |
| B5S50_RS17590 (B5S50_17400) | pilT | 3748798..3749853 (+) | 1056 | WP_065077504.1 | type IV pilus twitching motility protein PilT | Machinery gene |
Sequence
Protein
Download Length: 351 a.a. Molecular weight: 38796.24 Da Isoelectric Point: 8.5799
>NTDB_id=223589 B5S50_RS17590 WP_065077504.1 3748798..3749853(+) (pilT) [Clostridium sp. 001]
MLSLKDLLEITIKEEASDLHLTVGVPPVIRVNGELEVLGKEKLQPSDTERFSKEILDKDYDKYMKIGEIDISFSVAGLGR
FRVNIFKQRGSSAIAVRVVGLKIPTLNELKFPQVIKNLLSYKKGLILVTGPTGCGKSTTLAAMINEINCTRASHIITLED
PIEYLHKHNKSIINQREIGKDSISYANALKSVLREDPDVILVGEMRDLETISVVLTASETGHLVFSTLHTIGAAKTIDRI
VDVFPPYQQQQIKIQLAAVLRGIVSQQLIHREDGKGRVAAVETMIATPAIKNMIREGKTYQIESSMQTGSKYGMKTMDIA
LAELYKKGLISYESAIAYACDANIITKIISL
MLSLKDLLEITIKEEASDLHLTVGVPPVIRVNGELEVLGKEKLQPSDTERFSKEILDKDYDKYMKIGEIDISFSVAGLGR
FRVNIFKQRGSSAIAVRVVGLKIPTLNELKFPQVIKNLLSYKKGLILVTGPTGCGKSTTLAAMINEINCTRASHIITLED
PIEYLHKHNKSIINQREIGKDSISYANALKSVLREDPDVILVGEMRDLETISVVLTASETGHLVFSTLHTIGAAKTIDRI
VDVFPPYQQQQIKIQLAAVLRGIVSQQLIHREDGKGRVAAVETMIATPAIKNMIREGKTYQIESSMQTGSKYGMKTMDIA
LAELYKKGLISYESAIAYACDANIITKIISL
Nucleotide
Download Length: 1056 bp
>NTDB_id=223589 B5S50_RS17590 WP_065077504.1 3748798..3749853(+) (pilT) [Clostridium sp. 001]
TTGCTATCACTAAAAGATTTATTAGAAATAACTATTAAAGAAGAGGCATCAGATTTGCACCTTACTGTAGGGGTACCTCC
TGTAATAAGGGTAAATGGAGAACTTGAAGTACTTGGAAAGGAAAAACTACAGCCATCAGATACAGAAAGATTTAGTAAAG
AAATATTAGATAAAGATTATGATAAATATATGAAAATTGGAGAAATTGATATATCATTTTCTGTAGCTGGGCTTGGTAGA
TTTAGAGTAAATATTTTTAAGCAAAGGGGAAGTAGTGCCATTGCAGTGAGAGTTGTAGGATTGAAAATACCTACTTTAAA
TGAACTTAAATTTCCACAGGTAATAAAAAATCTTTTATCCTATAAAAAAGGACTGATTTTGGTAACAGGTCCTACAGGTT
GTGGAAAAAGTACAACTTTAGCAGCTATGATAAATGAAATAAATTGTACAAGAGCGTCACATATAATAACTCTAGAAGAT
CCTATAGAATATTTACATAAGCACAACAAGTCTATAATAAATCAGAGGGAAATAGGAAAAGATTCTATTAGCTATGCTAA
TGCCTTAAAGTCAGTGCTTAGAGAAGATCCAGATGTAATTTTAGTGGGAGAAATGAGAGACCTTGAAACTATATCTGTTG
TGCTTACGGCTTCTGAAACTGGACATCTGGTATTTTCGACGTTACATACTATAGGAGCAGCTAAAACTATAGATAGAATT
GTAGATGTATTTCCACCGTATCAACAACAGCAGATAAAAATACAATTAGCAGCAGTCTTAAGGGGAATAGTATCTCAGCA
GCTTATACATAGAGAAGATGGTAAGGGAAGAGTAGCAGCAGTTGAGACTATGATTGCCACTCCAGCCATTAAAAATATGA
TAAGAGAAGGAAAAACCTATCAAATAGAATCTTCAATGCAAACAGGATCAAAGTATGGTATGAAGACAATGGATATAGCA
CTGGCGGAACTTTATAAAAAAGGATTAATATCTTATGAATCTGCCATTGCCTATGCTTGTGATGCAAATATAATAACTAA
AATAATTTCTTTATAG
TTGCTATCACTAAAAGATTTATTAGAAATAACTATTAAAGAAGAGGCATCAGATTTGCACCTTACTGTAGGGGTACCTCC
TGTAATAAGGGTAAATGGAGAACTTGAAGTACTTGGAAAGGAAAAACTACAGCCATCAGATACAGAAAGATTTAGTAAAG
AAATATTAGATAAAGATTATGATAAATATATGAAAATTGGAGAAATTGATATATCATTTTCTGTAGCTGGGCTTGGTAGA
TTTAGAGTAAATATTTTTAAGCAAAGGGGAAGTAGTGCCATTGCAGTGAGAGTTGTAGGATTGAAAATACCTACTTTAAA
TGAACTTAAATTTCCACAGGTAATAAAAAATCTTTTATCCTATAAAAAAGGACTGATTTTGGTAACAGGTCCTACAGGTT
GTGGAAAAAGTACAACTTTAGCAGCTATGATAAATGAAATAAATTGTACAAGAGCGTCACATATAATAACTCTAGAAGAT
CCTATAGAATATTTACATAAGCACAACAAGTCTATAATAAATCAGAGGGAAATAGGAAAAGATTCTATTAGCTATGCTAA
TGCCTTAAAGTCAGTGCTTAGAGAAGATCCAGATGTAATTTTAGTGGGAGAAATGAGAGACCTTGAAACTATATCTGTTG
TGCTTACGGCTTCTGAAACTGGACATCTGGTATTTTCGACGTTACATACTATAGGAGCAGCTAAAACTATAGATAGAATT
GTAGATGTATTTCCACCGTATCAACAACAGCAGATAAAAATACAATTAGCAGCAGTCTTAAGGGGAATAGTATCTCAGCA
GCTTATACATAGAGAAGATGGTAAGGGAAGAGTAGCAGCAGTTGAGACTATGATTGCCACTCCAGCCATTAAAAATATGA
TAAGAGAAGGAAAAACCTATCAAATAGAATCTTCAATGCAAACAGGATCAAAGTATGGTATGAAGACAATGGATATAGCA
CTGGCGGAACTTTATAAAAAAGGATTAATATCTTATGAATCTGCCATTGCCTATGCTTGTGATGCAAATATAATAACTAA
AATAATTTCTTTATAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilT | Vibrio cholerae strain A1552 |
51.198 |
95.157 |
0.487 |
| pilT | Vibrio cholerae O1 biovar El Tor strain E7946 |
51.198 |
95.157 |
0.487 |
| pilT | Neisseria meningitidis 8013 |
48.397 |
97.721 |
0.473 |
| pilT | Acinetobacter baylyi ADP1 |
48.968 |
96.581 |
0.473 |
| pilT | Neisseria gonorrhoeae MS11 |
49.107 |
95.726 |
0.47 |
| pilT | Pseudomonas aeruginosa PAK |
48.521 |
96.296 |
0.467 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
46.552 |
99.145 |
0.462 |
| pilT | Acinetobacter baumannii strain A118 |
48.358 |
95.442 |
0.462 |
| pilT | Acinetobacter nosocomialis M2 |
48.358 |
95.442 |
0.462 |
| pilT | Acinetobacter baumannii D1279779 |
48.358 |
95.442 |
0.462 |
| pilT | Pseudomonas stutzeri DSM 10701 |
47.633 |
96.296 |
0.459 |
| pilT | Legionella pneumophila strain Lp02 |
46.377 |
98.291 |
0.456 |
| pilT | Legionella pneumophila strain ERS1305867 |
46.377 |
98.291 |
0.456 |
| pilU | Acinetobacter baylyi ADP1 |
40.698 |
98.006 |
0.399 |
| pilU | Pseudomonas stutzeri DSM 10701 |
39.886 |
100 |
0.399 |
| pilU | Vibrio cholerae strain A1552 |
39.942 |
97.721 |
0.39 |