Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A6J44_RS02695 Genome accession   NZ_CP020418
Coordinates   379649..380683 (-) Length   344 a.a.
NCBI ID   WP_082295372.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain FDAARGOS_205     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 374649..385683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J44_RS02675 (A6J44_02635) - 375228..375512 (+) 285 WP_003692093.1 GIY-YIG nuclease family protein -
  A6J44_RS02685 (A6J44_02645) yccS 375992..378142 (-) 2151 WP_003690198.1 YccS family putative transporter -
  A6J44_RS02690 (A6J44_02650) - 378259..379485 (-) 1227 WP_050159250.1 PilT/PilU family type 4a pilus ATPase -
  A6J44_RS02695 (A6J44_02655) pilT 379649..380683 (-) 1035 WP_082295372.1 type IV pilus twitching motility protein PilT Machinery gene
  A6J44_RS02700 (A6J44_02660) - 380803..381489 (+) 687 WP_003699139.1 YggS family pyridoxal phosphate-dependent enzyme -
  A6J44_RS02705 (A6J44_02665) - 381489..381896 (+) 408 WP_003690202.1 hypothetical protein -
  A6J44_RS02710 (A6J44_02670) proC 381927..382736 (+) 810 WP_226888057.1 pyrroline-5-carboxylate reductase -
  A6J44_RS02715 (A6J44_02675) dksA 382884..383300 (+) 417 WP_003692108.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37611.95 Da        Isoelectric Point: 6.8962

>NTDB_id=222521 A6J44_RS02695 WP_082295372.1 379649..380683(-) (pilT) [Neisseria gonorrhoeae strain FDAARGOS_205]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LVRQGLIAPEAARRRAQNSESMSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=222521 A6J44_RS02695 WP_082295372.1 379649..380683(-) (pilT) [Neisseria gonorrhoeae strain FDAARGOS_205]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGCCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGAGTTCCGCATTGCGCGAAGACCCAGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTCCATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACGATGGACCAATCG
CTGGTGCGCCAAGGGCTGATCGCACCGGAAGCCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

99.135

100

1
  pilT Neisseria meningitidis 8013

98.271

100

0.991
  pilT Acinetobacter baumannii D1279779

67.536

100

0.677
  pilT Acinetobacter baumannii strain A118

67.536

100

0.677
  pilT Acinetobacter nosocomialis M2

67.536

100

0.677
  pilT Acinetobacter baylyi ADP1

66.957

100

0.672
  pilT Pseudomonas stutzeri DSM 10701

66.667

100

0.669
  pilT Pseudomonas aeruginosa PAK

66.087

100

0.663
  pilT Legionella pneumophila strain Lp02

64.223

99.128

0.637
  pilT Legionella pneumophila strain ERS1305867

64.223

99.128

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.242

95.93

0.616
  pilT Vibrio cholerae strain A1552

64.242

95.93

0.616
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.097

98.547

0.523
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Acinetobacter baylyi ADP1

40.062

93.605

0.375
  pilU Vibrio cholerae strain A1552

39.628

93.895

0.372

Multiple sequence alignment