Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A6J40_RS06870 Genome accession   NZ_CP020412
Coordinates   1553450..1554484 (-) Length   344 a.a.
NCBI ID   WP_003632448.1    Uniprot ID   D3HQS5
Organism   Legionella longbeachae strain FDAARGOS_201     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1548450..1559484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J40_RS06860 (A6J40_06835) - 1549324..1550601 (-) 1278 WP_223800105.1 GSCFA domain-containing protein -
  A6J40_RS18770 - 1550761..1551063 (-) 303 Protein_1342 WcbI family polysaccharide biosynthesis putative acetyltransferase -
  A6J40_RS06865 (A6J40_06840) - 1551879..1553351 (+) 1473 WP_003632447.1 hypothetical protein -
  A6J40_RS06870 (A6J40_06845) pilT 1553450..1554484 (-) 1035 WP_003632448.1 type IV pilus twitching motility protein PilT Machinery gene
  A6J40_RS06875 (A6J40_06850) - 1554600..1555301 (+) 702 WP_003632449.1 YggS family pyridoxal phosphate-dependent enzyme -
  A6J40_RS06880 (A6J40_06855) - 1555422..1555997 (+) 576 WP_003632450.1 YggT family protein -
  A6J40_RS06885 (A6J40_06860) - 1556044..1556664 (+) 621 WP_003632451.1 DUF2845 domain-containing protein -
  A6J40_RS06890 (A6J40_06865) - 1556921..1557202 (+) 282 WP_012978921.1 hypothetical protein -
  A6J40_RS06895 (A6J40_06870) - 1557527..1557739 (+) 213 WP_012978920.1 hypothetical protein -
  A6J40_RS06900 (A6J40_06875) - 1558155..1559201 (-) 1047 WP_012978919.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38380.38 Da        Isoelectric Point: 6.9776

>NTDB_id=222435 A6J40_RS06870 WP_003632448.1 1553450..1554484(-) (pilT) [Legionella longbeachae strain FDAARGOS_201]
MDIAELLAFSVKNKSSDLHLSAGLPPMIRVDGDLRKINLPPLEHKEVIKIIYDVMNDRQRKEFEEFLEVDFSFEIENLSR
FRVNVFNQSRGAAAVFRTIPSLILGMKDLGLPSIFEEMASYPKGLVLVTGPTGSGKSTTLAAMIDYINSTRYDHILTVED
PIEFLHQSKKCLLNQREVHRDTLSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKAMVRSMLSESLQAVIAQTLLKKNGGGRVAALEIMICTGAIRNLIREDKVAQMYSSIQTGQAKGMQTLDQHL
KELVNKNIISRNTAHAVAFDKSLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=222435 A6J40_RS06870 WP_003632448.1 1553450..1554484(-) (pilT) [Legionella longbeachae strain FDAARGOS_201]
ATGGATATAGCAGAATTATTGGCATTTTCAGTCAAAAATAAATCTTCAGATCTACATTTGTCCGCGGGATTACCTCCAAT
GATTCGTGTTGATGGGGATTTACGCAAAATCAATTTGCCTCCTCTGGAGCATAAAGAGGTGATAAAAATTATTTATGATG
TAATGAACGATCGACAGAGAAAAGAGTTTGAAGAGTTTTTAGAAGTTGATTTTTCTTTTGAAATAGAAAATCTCTCCCGT
TTCAGGGTAAATGTATTTAATCAATCACGAGGTGCCGCAGCAGTTTTTCGTACTATTCCTTCTCTGATTTTAGGTATGAA
AGACTTAGGGCTACCATCTATTTTCGAAGAAATGGCAAGTTATCCCAAAGGATTGGTATTGGTCACAGGGCCCACAGGAT
CTGGGAAAAGTACAACCCTGGCTGCGATGATCGATTACATTAACTCAACTCGTTATGATCATATCTTAACCGTTGAAGAT
CCTATAGAGTTTCTTCACCAGAGTAAAAAATGTTTATTGAATCAGCGTGAAGTCCATCGGGATACGCTTAGCTTTAATGC
AGCTTTACGTTCTGCATTACGTGAAGATCCTGATGTTATTTTGGTAGGGGAGTTACGTGATTTGGAGACGATTCGTCTGG
CAATGACTGCAGCAGAGACAGGACATTTAGTATTTGGTACTTTGCATACTAATTCCGCCACTAAAACGATTAACCGTATT
ATTGACGTTTTTCCAGCAGAGGAAAAAGCGATGGTTCGTTCAATGCTGTCTGAGTCTTTGCAAGCAGTGATCGCTCAGAC
GTTGTTGAAAAAAAATGGAGGAGGGCGAGTAGCTGCTCTAGAAATAATGATCTGTACAGGAGCCATTCGTAACTTAATTC
GAGAAGATAAAGTCGCACAAATGTATTCTTCCATCCAAACAGGACAAGCTAAGGGAATGCAAACTTTAGATCAACATTTA
AAAGAATTAGTTAATAAAAATATTATTTCACGTAATACGGCCCATGCGGTTGCTTTTGATAAATCTTTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3HQS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

88.953

100

0.89
  pilT Legionella pneumophila strain Lp02

88.953

100

0.89
  pilT Acinetobacter baylyi ADP1

76.923

98.256

0.756
  pilT Acinetobacter baumannii strain A118

76.627

98.256

0.753
  pilT Acinetobacter nosocomialis M2

76.627

98.256

0.753
  pilT Acinetobacter baumannii D1279779

76.627

98.256

0.753
  pilT Pseudomonas aeruginosa PAK

76.647

97.093

0.744
  pilT Pseudomonas stutzeri DSM 10701

76.048

97.093

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.64

0.663
  pilT Vibrio cholerae strain A1552

69.301

95.64

0.663
  pilT Neisseria meningitidis 8013

66.567

97.384

0.648
  pilT Neisseria gonorrhoeae MS11

66.269

97.384

0.645
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.503
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

42.284

94.186

0.398
  pilU Acinetobacter baylyi ADP1

39.759

96.512

0.384

Multiple sequence alignment